Characterization of a self-assembled monolayer of thiol on a gold surface and the fabrication of a biosensor chip based on surface plasmon resonance for detecting anti-GAD antibody

Jung Wook Lee; Sang Jun Sim; Sung Min Cho; Jeewon Lee

doi:10.1016/j.bios.2004.04.017

Characterization of a self-assembled monolayer of thiol on a gold surface and the fabrication of a biosensor chip based on surface plasmon resonance for detecting anti-GAD antibody

Jung Wook Lee, Sang Jun Sim, Sung Min Cho, Jeewon Lee

Department of Chemical and Biological Engineering

Research output: Contribution to journal › Article › peer-review

116 Citations (Scopus)

Abstract

A biosensor chip utilizing surface plasmon resonance (SPR) was fabricated for detecting anti-glutamic acid decarboxylase (GAD) antibody, which is an indicator of the presence of type I diabetes mellitus. The sensor surfaces were constructed from various thiol mixtures of different molar ratios of 3-mercaptopropionic acid (3-MPA) to 11-mercaptoundecanoic acid (11-MUA). To determine the surface characteristics of the different alkanethiol monolayers, several quantitative and kinetic measurements were carried out. The extent of immobilization of streptavidin (SA) and biotin-GAD (the anti-GAD receptor) and the immune response of anti-GAD antibody against GAD were measured using the SPR biosensor. The terminal functional group of a thiol has different effects on the adsorption and covalent binding of protein depending on the steric hindrance. The protein chip described herein permits simple, rapid detection of anti-GAD antibody.

Original language	English
Pages (from-to)	1422-1427
Number of pages	6
Journal	Biosensors and Bioelectronics
Volume	20
Issue number	7
DOIs	https://doi.org/10.1016/j.bios.2004.04.017
Publication status	Published - 2005 Jan 15

Keywords

Glutamic acid decarboxylase
Immunosensor
Protein adsorption
Self-assembled monolayer
Streptavidin
Surface plasmon resonance

ASJC Scopus subject areas

Biotechnology
Biophysics
Biomedical Engineering
Electrochemistry

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.bios.2004.04.017

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Characterization of a self-assembled monolayer of thiol on a gold surface and the fabrication of a biosensor chip based on surface plasmon resonance for detecting anti-GAD antibody. / Lee, Jung Wook; Sim, Sang Jun; Cho, Sung Min et al.
In: Biosensors and Bioelectronics, Vol. 20, No. 7, 15.01.2005, p. 1422-1427.

Research output: Contribution to journal › Article › peer-review

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title = "Characterization of a self-assembled monolayer of thiol on a gold surface and the fabrication of a biosensor chip based on surface plasmon resonance for detecting anti-GAD antibody",

abstract = "A biosensor chip utilizing surface plasmon resonance (SPR) was fabricated for detecting anti-glutamic acid decarboxylase (GAD) antibody, which is an indicator of the presence of type I diabetes mellitus. The sensor surfaces were constructed from various thiol mixtures of different molar ratios of 3-mercaptopropionic acid (3-MPA) to 11-mercaptoundecanoic acid (11-MUA). To determine the surface characteristics of the different alkanethiol monolayers, several quantitative and kinetic measurements were carried out. The extent of immobilization of streptavidin (SA) and biotin-GAD (the anti-GAD receptor) and the immune response of anti-GAD antibody against GAD were measured using the SPR biosensor. The terminal functional group of a thiol has different effects on the adsorption and covalent binding of protein depending on the steric hindrance. The protein chip described herein permits simple, rapid detection of anti-GAD antibody.",

keywords = "Glutamic acid decarboxylase, Immunosensor, Protein adsorption, Self-assembled monolayer, Streptavidin, Surface plasmon resonance",

author = "Lee, {Jung Wook} and Sim, {Sang Jun} and Cho, {Sung Min} and Jeewon Lee",

note = "Funding Information: This research was supported by grants from the Korea Science and Engineering Foundation (KOSEF). We would like to thank Dr. S.H. Choi for her technical advice.",

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AU - Sim, Sang Jun

AU - Cho, Sung Min

AU - Lee, Jeewon

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N2 - A biosensor chip utilizing surface plasmon resonance (SPR) was fabricated for detecting anti-glutamic acid decarboxylase (GAD) antibody, which is an indicator of the presence of type I diabetes mellitus. The sensor surfaces were constructed from various thiol mixtures of different molar ratios of 3-mercaptopropionic acid (3-MPA) to 11-mercaptoundecanoic acid (11-MUA). To determine the surface characteristics of the different alkanethiol monolayers, several quantitative and kinetic measurements were carried out. The extent of immobilization of streptavidin (SA) and biotin-GAD (the anti-GAD receptor) and the immune response of anti-GAD antibody against GAD were measured using the SPR biosensor. The terminal functional group of a thiol has different effects on the adsorption and covalent binding of protein depending on the steric hindrance. The protein chip described herein permits simple, rapid detection of anti-GAD antibody.

AB - A biosensor chip utilizing surface plasmon resonance (SPR) was fabricated for detecting anti-glutamic acid decarboxylase (GAD) antibody, which is an indicator of the presence of type I diabetes mellitus. The sensor surfaces were constructed from various thiol mixtures of different molar ratios of 3-mercaptopropionic acid (3-MPA) to 11-mercaptoundecanoic acid (11-MUA). To determine the surface characteristics of the different alkanethiol monolayers, several quantitative and kinetic measurements were carried out. The extent of immobilization of streptavidin (SA) and biotin-GAD (the anti-GAD receptor) and the immune response of anti-GAD antibody against GAD were measured using the SPR biosensor. The terminal functional group of a thiol has different effects on the adsorption and covalent binding of protein depending on the steric hindrance. The protein chip described herein permits simple, rapid detection of anti-GAD antibody.

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KW - Immunosensor

KW - Protein adsorption

KW - Self-assembled monolayer

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KW - Surface plasmon resonance

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Characterization of a self-assembled monolayer of thiol on a gold surface and the fabrication of a biosensor chip based on surface plasmon resonance for detecting anti-GAD antibody

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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