Characterization of alfalfa mosaic virus capsid protein using Cryo-EM

Hyeongseop Jeong, Youngmin Park, Sooji Song, Kyungmin Min, Jae Sung Woo, Young Ho Lee, Eun Ju Sohn, Sangmin Lee

Research output: Contribution to journalArticlepeer-review

Abstract

VLPs are virus-like particles that comprise viral capsid proteins that can self-assemble and mimic the shape and size of real viral particles; however, because they do not contain genetic material they cannot infect host cells. VLPs have great potential as safe drug/vehicle candidates; therefore, they are gaining popularity in the field of preventive medicine and therapeutics. Indeed, extensive studies are underway to examine their role as carriers for immunization and as vehicles for delivery of therapeutic agents. Here, we examined the possibility of developing VLP-utilizing technology based on an efficient VLP production process and high-resolution structural analysis. Nicotiana benthamiana was used as an expression platform to produce the coat protein of the alfalfa mosaic virus (AMV-CP). About 250 mg/kg of rAMV-CP was produced from Nicotiana benthamiana leaves. Structural analysis revealed that the oligomeric status of rAMV-CP changed according to the composition and pH of the buffer. Size exclusion chromatography and electron microscopy analysis confirmed the optimal conditions for rAMV-CP VLP formation, and a 2.4 Å resolution structure was confirmed by cryo-EM analysis. Based on the efficient protein production, VLP manufacturing technology, and high-resolution structure presented herein, we suggest that rAMV-CP VLP is a useful platform for development of various new drugs.

Original languageEnglish
Pages (from-to)161-167
Number of pages7
JournalBiochemical and biophysical research communications
Volume559
DOIs
Publication statusPublished - 2021 Jun 25

Bibliographical note

Publisher Copyright:
© 2021 Elsevier Inc.

Keywords

  • Alfalfa mosaic virus
  • Coat protein
  • Cryo-EM
  • Plant
  • Virus like particle

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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