Characterization of antimicrobial resistance and quinolone resistance factors in high-level ciprofloxacin-resistant Enterococcus faecalis and Enterococcus faecium isolates obtained from fresh produce and fecal samples of patients

Min Chan Kim, Gun Jo Woo

    Research output: Contribution to journalArticlepeer-review

    21 Citations (Scopus)

    Abstract

    BACKGROUND: The emergence of fluoroquinolone-resistant enterococci is worldwide. Antimicrobial resistance was characterized and the effect of quinolone-resistance factors was analyzed in high-level ciprofloxacin-resistant (HLCR) Enterococcus faecalis and Enterococcus faecium isolated from fresh produce and fecal samples of patients. RESULTS: Among the 81 ciprofloxacin-resistant Enterococcus isolates, 46 showed high levels of ciprofloxacin resistance, resistance to other quinolone antibiotics, and multidrug resistance profiles. The virulence factors esp and hyl were identified in 27 (58.7%) and 25 (54.3%) of isolates, respectively. Sequence type analysis showed that 35 strains of HLCR E. faecium were clonal complex 17. Eleven strains of HLCR E. faecalis were confirmed as sequence type (ST) 28, ST 64 and ST 125. Quinolone resistance-determining region mutation was identified in HLCR Enterococcus isolates; with serine being changed in gyrA83, gyrA87 and parC80. This result shows that gyrA and parC mutations could be important factors for high-level resistance to fluoroquinolones. CONCLUSION: No significant differences were observed in antimicrobial resistance patterns and genetic characteristics among the isolates from fresh produce and fecal samples. Therefore, good agricultural practices in farming and continuous monitoring of patients, food and the environment for Enterococcus spp. should be performed to prevent antimicrobial resistance and enable reduction of resistance rates.

    Original languageEnglish
    Pages (from-to)2858-2864
    Number of pages7
    JournalJournal of the Science of Food and Agriculture
    Volume97
    Issue number9
    DOIs
    Publication statusPublished - 2017 Jul 1

    Bibliographical note

    Publisher Copyright:
    © 2016 Society of Chemical Industry

    Keywords

    • Enterococcus spp
    • gyrA
    • high-level ciprofloxacin resistance
    • parC
    • sequence type
    • virulence factor

    ASJC Scopus subject areas

    • Biotechnology
    • Food Science
    • Agronomy and Crop Science
    • Nutrition and Dietetics

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