Characterization of Brassica napus Flavonol Synthase Involved in Flavonol Biosynthesis in Brassica napus L.

Tien Thanh Vu, Chan Young Jeong, Hoai Nguyen Nguyen, Dongho Lee, Sang A. Lee, Ji Hye Kim, Suk Whan Hong, Hojoung Lee

    Research output: Contribution to journalArticlepeer-review

    35 Citations (Scopus)

    Abstract

    Recently, Brassica napus has become a very important crop for plant oil production. Flavonols, an uncolored flavonoid subclass, have a high antioxidative effect and are known to have antiproliferative, antiangiogenic, and neuropharmacological properties. In B. napus, some flavonoid structural genes have been identified, such as, BnF3H-1, BnCHS, and BnC4H-1. However, no studies on FLS genes in B. napus have been conducted. Thus, in this study, we cloned and characterized the function of BnFLS gene B. napus. By overexpression of the BnFLS gene, flavonol (kaempferol and quercetin) levels were recovered in the Arabidopsis atfls1-ko mutant. In addition, we found that the higher endogenous flavonol levels of BnFLS-ox in vitro shoots correlated with slightly higher ROS scavenging activities. Thus, our results indicate that the BnFLS gene encodes for a BnFLS enzyme that can be manipulated to specifically increase flavonol accumulation in oilseed plants and other species such as Arabidopsis.

    Original languageEnglish
    Pages (from-to)7819-7829
    Number of pages11
    JournalJournal of agricultural and food chemistry
    Volume63
    Issue number35
    DOIs
    Publication statusPublished - 2015 Sept 9

    Bibliographical note

    Publisher Copyright:
    © 2015 American Chemical Society.

    Keywords

    • BnFLS
    • Brassica napus
    • anthocyanin
    • flavonoids
    • flavonols
    • oilseed

    ASJC Scopus subject areas

    • General Chemistry
    • General Agricultural and Biological Sciences

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