Characterization of geranium (Pelargonium graveolens) chloroplast EF-Tu cDNA

Chul Joo Kang; Min Goo Lee; Young Sil Cho; Joong Won Lee; Yoon Jung Kyung; Jeong Sheop Shin; Eun Soo Kim; Jeong Kook Kim

doi:10.1007/s10059-000-0579-2

Characterization of geranium (Pelargonium graveolens) chloroplast EF-Tu cDNA

Chul Joo Kang, Min Goo Lee, Young Sil Cho, Joong Won Lee, Yoon Jung Kyung, Jeong Sheop Shin, Eun Soo Kim, Jeong Kook Kim

Research output: Contribution to journal › Article › peer-review

2 Citations (Scopus)

Abstract

A geranium (Pelargonium graveolens) chloroplast translational elongation factor EF-Tu (tufA) cDNA was isolated. The geranium tufA cDNA is 1,584 bp long with 20 bp of 5′ untranslated region (UTR) and 139 bp of 3′ UTR. It encodes 474 amino acids including a putative chloroplast transit peptide of 65 amino acids. The deduced polypeptides of the geranium tufA cDNA contains four GTP binding sequences in its N-terminal region and two chloroplast EF-Tu signature regions in the C-terminal region. The predicted molecular weight of the mature geranium chloroplast EF-Tu protein was about 45,000 and its amino acid sequence identity with the chloroplast EF-Tu proteins of tobacco, pea, Arabidopsis, rice, and soybean ranges from 85% to 91%. The geranium tufA appears to exist as a single copy gene like Arabidopsis and rice, whereas other known dicot plants have more than one copy in their nuclear genomes.

Original language	English
Pages (from-to)	579-583
Number of pages	5
Journal	Molecules and cells
Volume	10
Issue number	5
DOIs	https://doi.org/10.1007/s10059-000-0579-2
Publication status	Published - 2000 Oct 31

Bibliographical note

Funding Information:
Acknowledgments The authors wish to acknowledge the financial support of the Korean Research Foundation in the 1997 program year.

Keywords

Chloroplast
Elongation Factor EF-Tu
Geranium

ASJC Scopus subject areas

Molecular Biology
Cell Biology

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10.1007/s10059-000-0579-2

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title = "Characterization of geranium (Pelargonium graveolens) chloroplast EF-Tu cDNA",

abstract = "A geranium (Pelargonium graveolens) chloroplast translational elongation factor EF-Tu (tufA) cDNA was isolated. The geranium tufA cDNA is 1,584 bp long with 20 bp of 5′ untranslated region (UTR) and 139 bp of 3′ UTR. It encodes 474 amino acids including a putative chloroplast transit peptide of 65 amino acids. The deduced polypeptides of the geranium tufA cDNA contains four GTP binding sequences in its N-terminal region and two chloroplast EF-Tu signature regions in the C-terminal region. The predicted molecular weight of the mature geranium chloroplast EF-Tu protein was about 45,000 and its amino acid sequence identity with the chloroplast EF-Tu proteins of tobacco, pea, Arabidopsis, rice, and soybean ranges from 85% to 91%. The geranium tufA appears to exist as a single copy gene like Arabidopsis and rice, whereas other known dicot plants have more than one copy in their nuclear genomes.",

keywords = "Chloroplast, Elongation Factor EF-Tu, Geranium",

author = "Kang, {Chul Joo} and Lee, {Min Goo} and Cho, {Young Sil} and Lee, {Joong Won} and Kyung, {Yoon Jung} and Shin, {Jeong Sheop} and Kim, {Eun Soo} and Kim, {Jeong Kook}",

note = "Funding Information: Acknowledgments The authors wish to acknowledge the financial support of the Korean Research Foundation in the 1997 program year.",

year = "2000",

month = oct,

day = "31",

doi = "10.1007/s10059-000-0579-2",

language = "English",

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T1 - Characterization of geranium (Pelargonium graveolens) chloroplast EF-Tu cDNA

AU - Kang, Chul Joo

AU - Lee, Min Goo

AU - Cho, Young Sil

AU - Lee, Joong Won

AU - Kyung, Yoon Jung

AU - Shin, Jeong Sheop

AU - Kim, Eun Soo

AU - Kim, Jeong Kook

N1 - Funding Information: Acknowledgments The authors wish to acknowledge the financial support of the Korean Research Foundation in the 1997 program year.

PY - 2000/10/31

Y1 - 2000/10/31

N2 - A geranium (Pelargonium graveolens) chloroplast translational elongation factor EF-Tu (tufA) cDNA was isolated. The geranium tufA cDNA is 1,584 bp long with 20 bp of 5′ untranslated region (UTR) and 139 bp of 3′ UTR. It encodes 474 amino acids including a putative chloroplast transit peptide of 65 amino acids. The deduced polypeptides of the geranium tufA cDNA contains four GTP binding sequences in its N-terminal region and two chloroplast EF-Tu signature regions in the C-terminal region. The predicted molecular weight of the mature geranium chloroplast EF-Tu protein was about 45,000 and its amino acid sequence identity with the chloroplast EF-Tu proteins of tobacco, pea, Arabidopsis, rice, and soybean ranges from 85% to 91%. The geranium tufA appears to exist as a single copy gene like Arabidopsis and rice, whereas other known dicot plants have more than one copy in their nuclear genomes.

AB - A geranium (Pelargonium graveolens) chloroplast translational elongation factor EF-Tu (tufA) cDNA was isolated. The geranium tufA cDNA is 1,584 bp long with 20 bp of 5′ untranslated region (UTR) and 139 bp of 3′ UTR. It encodes 474 amino acids including a putative chloroplast transit peptide of 65 amino acids. The deduced polypeptides of the geranium tufA cDNA contains four GTP binding sequences in its N-terminal region and two chloroplast EF-Tu signature regions in the C-terminal region. The predicted molecular weight of the mature geranium chloroplast EF-Tu protein was about 45,000 and its amino acid sequence identity with the chloroplast EF-Tu proteins of tobacco, pea, Arabidopsis, rice, and soybean ranges from 85% to 91%. The geranium tufA appears to exist as a single copy gene like Arabidopsis and rice, whereas other known dicot plants have more than one copy in their nuclear genomes.

KW - Chloroplast

KW - Elongation Factor EF-Tu

KW - Geranium

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DO - 10.1007/s10059-000-0579-2

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AN - SCOPUS:0034739522

SN - 1016-8478

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JO - Molecules and cells

JF - Molecules and cells

IS - 5

ER -

Characterization of geranium (Pelargonium graveolens) chloroplast EF-Tu cDNA

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

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