Characterization of the full-length sequences of phospholipase A2 induced during flower development

Ji Young Kim; Young Soo Chung; Sung Han Ok; Sang Gak Lee; Won Il Chung; Ick Young Kim; Jeong Sheop Shin

doi:10.1016/S0167-4781(99)00193-1

Characterization of the full-length sequences of phospholipase A₂ induced during flower development

Ji Young Kim, Young Soo Chung, Sung Han Ok, Sang Gak Lee, Won Il Chung, Ick Young Kim, Jeong Sheop Shin

Research output: Contribution to journal › Article › peer-review

45 Citations (Scopus)

Abstract

The suppression subtractive hybridization (SSH) method was used to isolate developmentally regulated genes during carnation flower maturation. Carnation flower maturation-related clones obtained by the SSH were serially assigned as CFMI (carnation flower maturation-induced) clones. Northern blot analysis showed that several CFMI clones were differentially expressed during flower development. One of the clones, CFMI-3, showed similarity to various animal secretory phospholipases A₂ (PLA₂). Since little is known about PLA₂ gene sequence in plant species, the CFMI-3 clone was selected for further characterization by sequence analysis. Full sequence analysis reveals that the CFMI-3 contains a Ca²⁺ binding domain, a PLA₂ active site, and 12 conserved Cys residues, which is a distinct characteristic of PLA₂. Amino acid sequence alignment of CFMI-3 to various putative plant PLA₂ confirmed that the CFMI-3 cDNA is the full-length putative PLA₂ cDNA identified in plant species. Copyright (C) 1999 Elsevier Science B.V.

Original language	English
Pages (from-to)	389-392
Number of pages	4
Journal	Biochimica et Biophysica Acta - Gene Structure and Expression
Volume	1489
Issue number	2-3
DOIs	https://doi.org/10.1016/S0167-4781(99)00193-1
Publication status	Published - 1999 Dec 23

Keywords

Carnation flower
Differential gene expression
Flower maturation
Phospholipase A
Suppression subtractive hybridization

ASJC Scopus subject areas

Structural Biology
Biophysics
Biochemistry
Genetics

Access to Document

10.1016/S0167-4781(99)00193-1

Cite this

@article{c2fe640970f04f9f8e7d78ab5195306b,

title = "Characterization of the full-length sequences of phospholipase A2 induced during flower development",

abstract = "The suppression subtractive hybridization (SSH) method was used to isolate developmentally regulated genes during carnation flower maturation. Carnation flower maturation-related clones obtained by the SSH were serially assigned as CFMI (carnation flower maturation-induced) clones. Northern blot analysis showed that several CFMI clones were differentially expressed during flower development. One of the clones, CFMI-3, showed similarity to various animal secretory phospholipases A2 (PLA2). Since little is known about PLA2 gene sequence in plant species, the CFMI-3 clone was selected for further characterization by sequence analysis. Full sequence analysis reveals that the CFMI-3 contains a Ca2+ binding domain, a PLA2 active site, and 12 conserved Cys residues, which is a distinct characteristic of PLA2. Amino acid sequence alignment of CFMI-3 to various putative plant PLA2 confirmed that the CFMI-3 cDNA is the full-length putative PLA2 cDNA identified in plant species. Copyright (C) 1999 Elsevier Science B.V.",

keywords = "Carnation flower, Differential gene expression, Flower maturation, Phospholipase A, Suppression subtractive hybridization",

author = "Kim, {Ji Young} and Chung, {Young Soo} and Ok, {Sung Han} and Lee, {Sang Gak} and Chung, {Won Il} and Kim, {Ick Young} and Shin, {Jeong Sheop}",

note = "Funding Information: This research was funded by the MAF-SGRF (Ministry of Agriculture and Forestry Special Grants Research Program) in Korea. We thank Dr. Sangduk Kim for her stimulating discussions and helpful comments on the manuscript. ",

year = "1999",

month = dec,

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doi = "10.1016/S0167-4781(99)00193-1",

language = "English",

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AU - Kim, Ji Young

AU - Chung, Young Soo

AU - Ok, Sung Han

AU - Lee, Sang Gak

AU - Chung, Won Il

AU - Kim, Ick Young

AU - Shin, Jeong Sheop

N1 - Funding Information: This research was funded by the MAF-SGRF (Ministry of Agriculture and Forestry Special Grants Research Program) in Korea. We thank Dr. Sangduk Kim for her stimulating discussions and helpful comments on the manuscript.

PY - 1999/12/23

Y1 - 1999/12/23

N2 - The suppression subtractive hybridization (SSH) method was used to isolate developmentally regulated genes during carnation flower maturation. Carnation flower maturation-related clones obtained by the SSH were serially assigned as CFMI (carnation flower maturation-induced) clones. Northern blot analysis showed that several CFMI clones were differentially expressed during flower development. One of the clones, CFMI-3, showed similarity to various animal secretory phospholipases A2 (PLA2). Since little is known about PLA2 gene sequence in plant species, the CFMI-3 clone was selected for further characterization by sequence analysis. Full sequence analysis reveals that the CFMI-3 contains a Ca2+ binding domain, a PLA2 active site, and 12 conserved Cys residues, which is a distinct characteristic of PLA2. Amino acid sequence alignment of CFMI-3 to various putative plant PLA2 confirmed that the CFMI-3 cDNA is the full-length putative PLA2 cDNA identified in plant species. Copyright (C) 1999 Elsevier Science B.V.

AB - The suppression subtractive hybridization (SSH) method was used to isolate developmentally regulated genes during carnation flower maturation. Carnation flower maturation-related clones obtained by the SSH were serially assigned as CFMI (carnation flower maturation-induced) clones. Northern blot analysis showed that several CFMI clones were differentially expressed during flower development. One of the clones, CFMI-3, showed similarity to various animal secretory phospholipases A2 (PLA2). Since little is known about PLA2 gene sequence in plant species, the CFMI-3 clone was selected for further characterization by sequence analysis. Full sequence analysis reveals that the CFMI-3 contains a Ca2+ binding domain, a PLA2 active site, and 12 conserved Cys residues, which is a distinct characteristic of PLA2. Amino acid sequence alignment of CFMI-3 to various putative plant PLA2 confirmed that the CFMI-3 cDNA is the full-length putative PLA2 cDNA identified in plant species. Copyright (C) 1999 Elsevier Science B.V.

KW - Carnation flower

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KW - Flower maturation

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KW - Suppression subtractive hybridization

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