TY - JOUR
T1 - Characterization of tula virus from common voles (Microtus arvalis) in Poland
T2 - Evidence for geographic-specific phylogenetic clustering
AU - Song, Jin Won
AU - Baek, Luck Ju
AU - Song, Ki Joon
AU - Skrok, Anna
AU - Markowski, Janusz
AU - Bratosiewicz-Wasik, Jolanta
AU - Kordek, Radzislaw
AU - Liberski, Pawel P.
AU - Yanagihara, Richard
N1 - Funding Information:
This research was supported in part by grants from the Health Technology Planning and Evaluation Board, Ministry of Health and Welfare, Korea (03-PJ1-PG3-20200-0006), the Brain Korea 21 Program, and the U.S. Public Health Service (G12RR003061) from the Research Centers in Minority Institutions Program of the National Center for Research Resources, National Institutes of Health.
PY - 2004/10
Y1 - 2004/10
N2 - Tula virus (TULV), a recently identified arvicolid rodent-borne hantavirus, is harbored by the European common vole (Microtus arvalis) in Central Russia and the Czech and Slovak Republics. We report the isolation and characterization of this hantavirus from M. arvalis captured in Poland, a country where human disease caused by hantaviruses has not been recognized. Of 34 arvicolid rodents (24 Clethrionomys glareolus, 9 M. arvalis, 1 Pitymys sp.) captured in Lodz and Tuszyn, Poland, during June to September 1995, sera from 3 M. arvalis and 3 C. glareolus contained IgG antibodies to Puumala virus (PUUV), as determined by an indirect immunofluorescent antibody assay. Alignment and comparison of the 1852-nucleotide S segment and a 1676-nucleotide region of the G2 glycoprotein-encoding M segment, amplified from lung tissues of two hantavirus-seropositive M. arvalis, revealed 83.9-85.2% and 82.3-83.5% sequence similarity, respectively, with TULV strains from Central Russia and the Czech and Slovak Republics. A > 98% sequence conservation was found at the amino acid level. Phylogenetic analysis indicated that the newly found TULV strains from Poland were closely related to, but distinct from, TULV from elsewhere in Europe.
AB - Tula virus (TULV), a recently identified arvicolid rodent-borne hantavirus, is harbored by the European common vole (Microtus arvalis) in Central Russia and the Czech and Slovak Republics. We report the isolation and characterization of this hantavirus from M. arvalis captured in Poland, a country where human disease caused by hantaviruses has not been recognized. Of 34 arvicolid rodents (24 Clethrionomys glareolus, 9 M. arvalis, 1 Pitymys sp.) captured in Lodz and Tuszyn, Poland, during June to September 1995, sera from 3 M. arvalis and 3 C. glareolus contained IgG antibodies to Puumala virus (PUUV), as determined by an indirect immunofluorescent antibody assay. Alignment and comparison of the 1852-nucleotide S segment and a 1676-nucleotide region of the G2 glycoprotein-encoding M segment, amplified from lung tissues of two hantavirus-seropositive M. arvalis, revealed 83.9-85.2% and 82.3-83.5% sequence similarity, respectively, with TULV strains from Central Russia and the Czech and Slovak Republics. A > 98% sequence conservation was found at the amino acid level. Phylogenetic analysis indicated that the newly found TULV strains from Poland were closely related to, but distinct from, TULV from elsewhere in Europe.
KW - Hantavirus
KW - Microtus
KW - Poland
KW - phylogenetic analysis
KW - rodents
UR - http://www.scopus.com/inward/record.url?scp=4544250904&partnerID=8YFLogxK
U2 - 10.1023/B:VIRU.0000036384.50102.cf
DO - 10.1023/B:VIRU.0000036384.50102.cf
M3 - Article
C2 - 15284484
AN - SCOPUS:4544250904
SN - 0920-8569
VL - 29
SP - 239
EP - 247
JO - Virus Genes
JF - Virus Genes
IS - 2
ER -
