Characterization of two frizzled8 homologues expressed in the shield and prechordal plate of zebrafish embryos

Seok Hyung Kim, Hae Chul Park, Sang Yeob Yeo, Sung Kook Hong, Jang Won Choi, Cheol Hee Kim, Brant M. Weinstein, Tae Lin Huh

Research output: Contribution to journalArticlepeer-review

28 Citations (Scopus)


We have isolated and characterized two complete cDNA clones, Zfz8a and Zfz8b, which encode zebrafish Frizzled (Fz) homologues. The predicted protein sequences, spanning 579 and 576 amino acid residues for ZFz8a and ZFz8b, respectively, were highly homologous (78%) to each other and contained an extracellular cysteine-rich domain and seven transmembrane domains that are well conserved in Fz receptor protein members. In comparison with other Fz family members, ZFz8a and ZFz8b showed the highest homology with mouse Fz8 (MFz8), sharing 84 and 76% amino acid identity, respectively. The presence of Zfz8a and Zfz8b transcripts was detected by in situ hybridization in zebrafish embryos from the 512 cell stage, and their appearance in the future dorsal region could be observed before embryos reached the 30% epiboly stage. At shield stage, Zfz8a transcripts were expressed in both epiblast and shield whereas expression of Zfz8b was only detected in the embryonic shield. During gastrula stages, both Zfz8a and Zfz8b transcripts were found in anterior dorsal regions of the involuting mesendoderm (future prechordal plate). By the 2- to 3-somite stage, expression of both Zfz8a and Zfz8b was restricted to the prechordal plate and prospective anterior neurectoderm, although expression of the Zfz8a gene was no longer present in the most anterior portion of the prechordal plate, the polster. In one-eyed pinhead mutant embryos, which lack prechordal plate, both Zfz8a and Zfz8b transcripts were reduced, confirming the prechordal plate specificity of Zfz8a and Zfz8b gene expression. These results provide an additional evidence supporting the role of Wnt signaling in organizer-mediated axial patterning.

Original languageEnglish
Pages (from-to)193-198
Number of pages6
JournalMechanisms of Development
Issue number1-2
Publication statusPublished - 1998
Externally publishedYes

Bibliographical note

Funding Information:
We are grateful to Dr. Igor Dawid and Dr. Reiko Toyama for helpful discussions. We thank Dr. Ajay B. Chitnis and for a critical reading of the manuscript. This work was supported by the 1996 Future Oriented Generic Technology Grant from the Ministry of Science and Technology, Korea.


  • Axial mesoderm
  • Frizzled (fz)
  • Goosecoid (gsc)
  • Hypoblast
  • Neurectoderm
  • One-eyed pinhead (oep)
  • Polster
  • Prechordal plate
  • Pronephric duct
  • Shield
  • Wnt
  • Zebrafish
  • pax2

ASJC Scopus subject areas

  • Embryology
  • Developmental Biology


Dive into the research topics of 'Characterization of two frizzled8 homologues expressed in the shield and prechordal plate of zebrafish embryos'. Together they form a unique fingerprint.

Cite this