Characterizing DNA methylation patterns in pancreatic cancer genome

Aik Choon Tan; Antonio Jimeno; Steven H. Lin; Jenna Wheelhouse; Fonda Chan; Anna Solomon; N. V. Rajeshkumar; Belen Rubio-Viqueira; Manuel Hidalgo

doi:10.1016/j.molonc.2009.03.004

Characterizing DNA methylation patterns in pancreatic cancer genome

Aik Choon Tan, Antonio Jimeno, Steven H. Lin, Jenna Wheelhouse, Fonda Chan, Anna Solomon, N. V. Rajeshkumar, Belen Rubio-Viqueira, Manuel Hidalgo

Department of Computer Science and Engineering

Research output: Contribution to journal › Article › peer-review

126 Citations (Scopus)

Abstract

We performed a global methylation profiling assay on 1505 CpG sites across 807 genes to characterize DNA methylation patterns in pancreatic cancer genome. We found 289 CpG sites that were differentially methylated in normal pancreas, pancreatic tumors and cancer cell lines. We identified 23 and 35 candidate genes that are regulated by hypermethylation and hypomethylation in pancreatic cancer, respectively. We also identified candidate methylation markers that alter the expression of genes critical to gemcitabine susceptibility in pancreatic cancer. These results indicate that aberrant DNA methylation is a frequent epigenetic event in pancreatic cancer; and by using global methylation profiling assay, it is possible to identify these markers for diagnostic and therapeutic purposes in this disease.

Original language	English
Pages (from-to)	425-438
Number of pages	14
Journal	Molecular Oncology
Volume	3
Issue number	5-6
DOIs	https://doi.org/10.1016/j.molonc.2009.03.004
Publication status	Published - 2009 Dec

Bibliographical note

Funding Information:
Supported in part by the Sol Golman Center for Pancreatic Cancer Research, the Viragh Family Foundation and the Lee family.

Keywords

DNA methylation
Gemcitabine
Gene expression
Hypermethylation
Hypomethylation
Pancreatic cancer

ASJC Scopus subject areas

Molecular Medicine
Genetics
Oncology
Cancer Research

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.molonc.2009.03.004

Cite this

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title = "Characterizing DNA methylation patterns in pancreatic cancer genome",

abstract = "We performed a global methylation profiling assay on 1505 CpG sites across 807 genes to characterize DNA methylation patterns in pancreatic cancer genome. We found 289 CpG sites that were differentially methylated in normal pancreas, pancreatic tumors and cancer cell lines. We identified 23 and 35 candidate genes that are regulated by hypermethylation and hypomethylation in pancreatic cancer, respectively. We also identified candidate methylation markers that alter the expression of genes critical to gemcitabine susceptibility in pancreatic cancer. These results indicate that aberrant DNA methylation is a frequent epigenetic event in pancreatic cancer; and by using global methylation profiling assay, it is possible to identify these markers for diagnostic and therapeutic purposes in this disease.",

keywords = "DNA methylation, Gemcitabine, Gene expression, Hypermethylation, Hypomethylation, Pancreatic cancer",

author = "Tan, {Aik Choon} and Antonio Jimeno and Lin, {Steven H.} and Jenna Wheelhouse and Fonda Chan and Anna Solomon and Rajeshkumar, {N. V.} and Belen Rubio-Viqueira and Manuel Hidalgo",

note = "Funding Information: Supported in part by the Sol Golman Center for Pancreatic Cancer Research, the Viragh Family Foundation and the Lee family. ",

year = "2009",

month = dec,

doi = "10.1016/j.molonc.2009.03.004",

language = "English",

volume = "3",

pages = "425--438",

journal = "Molecular Oncology",

issn = "1574-7891",

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T1 - Characterizing DNA methylation patterns in pancreatic cancer genome

AU - Tan, Aik Choon

AU - Jimeno, Antonio

AU - Lin, Steven H.

AU - Wheelhouse, Jenna

AU - Chan, Fonda

AU - Solomon, Anna

AU - Rajeshkumar, N. V.

AU - Rubio-Viqueira, Belen

AU - Hidalgo, Manuel

N1 - Funding Information: Supported in part by the Sol Golman Center for Pancreatic Cancer Research, the Viragh Family Foundation and the Lee family.

PY - 2009/12

Y1 - 2009/12

N2 - We performed a global methylation profiling assay on 1505 CpG sites across 807 genes to characterize DNA methylation patterns in pancreatic cancer genome. We found 289 CpG sites that were differentially methylated in normal pancreas, pancreatic tumors and cancer cell lines. We identified 23 and 35 candidate genes that are regulated by hypermethylation and hypomethylation in pancreatic cancer, respectively. We also identified candidate methylation markers that alter the expression of genes critical to gemcitabine susceptibility in pancreatic cancer. These results indicate that aberrant DNA methylation is a frequent epigenetic event in pancreatic cancer; and by using global methylation profiling assay, it is possible to identify these markers for diagnostic and therapeutic purposes in this disease.

AB - We performed a global methylation profiling assay on 1505 CpG sites across 807 genes to characterize DNA methylation patterns in pancreatic cancer genome. We found 289 CpG sites that were differentially methylated in normal pancreas, pancreatic tumors and cancer cell lines. We identified 23 and 35 candidate genes that are regulated by hypermethylation and hypomethylation in pancreatic cancer, respectively. We also identified candidate methylation markers that alter the expression of genes critical to gemcitabine susceptibility in pancreatic cancer. These results indicate that aberrant DNA methylation is a frequent epigenetic event in pancreatic cancer; and by using global methylation profiling assay, it is possible to identify these markers for diagnostic and therapeutic purposes in this disease.

KW - DNA methylation

KW - Gemcitabine

KW - Gene expression

KW - Hypermethylation

KW - Hypomethylation

KW - Pancreatic cancer

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DO - 10.1016/j.molonc.2009.03.004

M3 - Article

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AN - SCOPUS:70350574158

SN - 1574-7891

VL - 3

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EP - 438

JO - Molecular Oncology

JF - Molecular Oncology

IS - 5-6

ER -

Characterizing DNA methylation patterns in pancreatic cancer genome

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

UN SDGs

Access to Document

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