Charge-Based Isolation of Extracellular Vesicles from Human Plasma

Woojin Back, Minseo Bang, Jik Han Jung, Ka Won Kang, Byeong Hyeon Choi, Yeonho Choi, Sunghoi Hong, Hyun Koo Kim, Yong Park, Ji Ho Park

Research output: Contribution to journalArticlepeer-review


Extracellular vesicles (EVs) have garnered significant attention due to their potential applications in disease diagnostics and management. However, the process of isolating EVs, primarily from blood samples, is still suboptimal. This is mainly attributed to the abundant nature of soluble proteins and lipoproteins, which are often separated together with EVs in the end products of conventional isolation methods. As such, we devise a single-step charge-based EV isolation method by utilizing positively charged beads to selectively remove negatively charged major impurities from human plasma via electrostatic interaction. By carefully controlling the buffer pH, we successfully collected EVs from undesired plasma components with superior purity and yield compared to conventional EV collection methods. Moreover, the developed process is rapid, taking only about 20 min for overall EV isolation. The charge-based isolation can ultimately benefit the EV-based liquid biopsy field for the early diagnosis of various diseases.

Original languageEnglish
Pages (from-to)17832-17838
Number of pages7
JournalACS Omega
Issue number16
Publication statusPublished - 2024 Apr 23

Bibliographical note

Publisher Copyright:
© 2024 The Authors. Published by American Chemical Society.

ASJC Scopus subject areas

  • General Chemistry
  • General Chemical Engineering


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