Chemical characteristics and enhanced hepatoprotective activities of Maillard reaction products derived from milk protein-sugar system

Nam Su Oh, Ji Young Lee, Hyun Ah Lee, Jae Yeon Joung, Yong Kook Shin, Sae Hun Kim, Younghoon Kim, Kwang Won Lee

Research output: Contribution to journalArticlepeer-review

35 Citations (Scopus)


The objective of this study was to investigate the characteristics, antioxidative properties, and hepatoprotective effects of Maillard reaction products (MRP) from milk protein reacted with sugars. The MRP were obtained from milk protein, whey protein concentrates and sodium caseinate, using 2 types of sugars, lactose and glucose, by heating the mixture at 55°C for 7. d in a sodium phosphate buffer (pH 7.4). Changes in the chemical modification of the milk protein were monitored by measuring the protein-bound carbonyls and PAGE protein profiles. The results showed that the amount of protein-bound carbonyls increased after Maillard reaction (MR). In addition, sodium dodecyl sulfate-PAGE analysis indicated a formation of high-molecular weight complexes through MR. The modification sites induced by MR of milk protein were monitored by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry analysis of tryptic-digested gel spots of MRP. As a result, modification and their localization in AA sequence of MRP was identified. Also, the MRP showed higher antioxidant activities than the intact milk protein, and they reduced intracellular reactive oxygen species production and inhibited the depletion of the reduced glutathione concentrations in the HepG2 cells. In particular, glucose-sodium caseinate MRP showed the highest biological activities among all MRP. Therefore, these results suggest that the MRP from milk protein reacting with sugars possess effective antioxidant activity and have a protective ability against oxidative damage.

Original languageEnglish
Pages (from-to)947-958
Number of pages12
JournalJournal of Dairy Science
Issue number2
Publication statusPublished - 2016 Feb 1

Bibliographical note

Publisher Copyright:
© 2016 American Dairy Science Association.


  • Antioxidant activity
  • Hepatoprotective effect
  • Maillard reaction
  • Milk protein

ASJC Scopus subject areas

  • Food Science
  • Animal Science and Zoology
  • Genetics


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