Chemical constituents from Neopestalotiopsis clavispora culture medium with estrogenic effects in MCF-7 cells

Haeun Kwon, Quynh Nhu Nguyen, Seung Mok Ryu, Jaeyoung Kwon, Sojung Park, Yuanqiang Guo, Bang Yeon Hwang, Joung Han Yim, Jae Jin Kim, Ki Sung Kang, Dongho Lee

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)


Estrogen is a vital hormone responsible for the development of the female reproductive system. Hence, estrogen deficiency in menopause increases various symptoms. This study aimed to discover biologically active compounds from an EtOAc-extract of Neopestalotiopsis clavispora culture medium which has previously shown estrogenic activity. A new α-pyrone (1) and a new tetramic acid derivative (2), together with hymenosetin (3) and pestalotiollide B (4), were isolated from the extract. The chemical structures of the new compounds were elucidated using MS and NMR spectroscopy, and the absolute configurations were established by quantum mechanical calculations of electronic circular dichroism and gauge-including atomic orbital NMR chemical shift, followed by DP4 + analysis. The isolated compounds were initially tested for their estrogenic activities using MCF-7 estrogen responsive human breast cancer cells. Hymenosetin (3) showed estrogenic activity by increasing the proliferation of MCF-7 cells at the concentration of 2.5 μM via the phosphorylation of estrogen receptor-α.

Original languageEnglish
Pages (from-to)134-137
Number of pages4
JournalPhytochemistry Letters
Publication statusPublished - 2022 Dec

Bibliographical note

Publisher Copyright:
© 2022 Phytochemical Society of Europe


  • A new tetramic acid derivative
  • A new α-pyrone
  • Estrogenic activity
  • Neopestalotiopsis clavispora

ASJC Scopus subject areas

  • Biotechnology
  • Biochemistry
  • Agronomy and Crop Science
  • Plant Science


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