TY - JOUR
T1 - Chemical-specific continuous biomonitoring using a recombinant bioluminescent bacterium DNT5 (nagR-nagAa::luxCDABE)
AU - Lee, Jin Hyung
AU - Mitchell, Robert J.
AU - Gu, Man Bock
N1 - Funding Information:
This research was supported by the Korea Science and Engineering Foundation (KOSEF) through the ADvanced Environmental Monitoring Research Center (ADEMRC) at GIST. We are grateful for the support.
PY - 2007/9/15
Y1 - 2007/9/15
N2 - The recombinant bioluminescent bacterium, DNT5, containing a nagR-nagAa::luxCDABE fusion, was tested in a multi-channel continuous monitoring system to evaluate its ability to detect benzoic acid derivatives. Seven chemicals, benzoic acid, salicylic acid, 2,5-dihydroxy benzoic acid, 3,5-dihydroxy benzoic acid, benzene, naphthalene and phenol, were used to characterize the responses of DNT5. This strain responded uniquely to each chemical, and these responses were then evaluated based upon the structures of each chemical. The greatest bioluminescent responses were to salicylic acid and benzoic acid, followed by 2,5-dihydroxy benzoic acid and 3,5-dihydroxy benzoic acid, but DNT5 was unresponsive when exposed to benzene, phenol and naphthalene, suggesting it has a strong preference for benzoic acid derivatives with few or no ring-substituted groups.
AB - The recombinant bioluminescent bacterium, DNT5, containing a nagR-nagAa::luxCDABE fusion, was tested in a multi-channel continuous monitoring system to evaluate its ability to detect benzoic acid derivatives. Seven chemicals, benzoic acid, salicylic acid, 2,5-dihydroxy benzoic acid, 3,5-dihydroxy benzoic acid, benzene, naphthalene and phenol, were used to characterize the responses of DNT5. This strain responded uniquely to each chemical, and these responses were then evaluated based upon the structures of each chemical. The greatest bioluminescent responses were to salicylic acid and benzoic acid, followed by 2,5-dihydroxy benzoic acid and 3,5-dihydroxy benzoic acid, but DNT5 was unresponsive when exposed to benzene, phenol and naphthalene, suggesting it has a strong preference for benzoic acid derivatives with few or no ring-substituted groups.
KW - Benzoic acid derivatives
KW - Chemical-specific biomonitoring
KW - Continuous monitoring
KW - Multi-channel continuous monitoring system
KW - Recombinant bioluminescent bacterium
KW - nagR promoter
UR - http://www.scopus.com/inward/record.url?scp=34548396389&partnerID=8YFLogxK
U2 - 10.1016/j.jbiotec.2007.06.020
DO - 10.1016/j.jbiotec.2007.06.020
M3 - Article
C2 - 17716769
AN - SCOPUS:34548396389
SN - 0168-1656
VL - 131
SP - 330
EP - 334
JO - Journal of Biotechnology
JF - Journal of Biotechnology
IS - 3
ER -