Chemopreventive Properties of Genipin on AGS Cell Line via Induction of JNK/Nrf2/ARE Signaling Pathway

Jee Min Kim, Hyeonseok Ko, Sun Joong Kim, So Hee Shim, Chang Hoon Ha, Hyo Ihl Chang

    Research output: Contribution to journalArticlepeer-review

    29 Citations (Scopus)

    Abstract

    Roles of dietary phytochemicals in cancer chemoprevention via induction of nuclear factor-erythroid-2-related factor 2 (Nrf2)-mediated antioxidant enzymes have been well established in a number of studies. In this study, FACS analysis was used to reveal that the intracellular reactive oxygen species level decreased at 0-25 μM of genipin treatment. Furthermore, immunofluorescence analysis and Western blotting were used to demonstrate that genipin treatment resulted in the upregulation and nuclear translocation of Nrf2, as well as upregulation of gastrointestinal glutathione peroxidase. Finally, we found that C-Jun-NH2-kinase (JNK) was also dose-dependently activated, where depleting JNK by using a biochemical inhibitor indicated that JNK was upstream of Nrf2. Interestingly, the antioxidant effects were limited to the treatment in the lower dosage of genipin, where higher dosage of genipin treatment resulted in the increased reactive oxygen species level and cytotoxicity. Thus, this study demonstrates for the first time that lower dosage of genipin results in the induction of JNK/Nrf2/ARE signaling pathway and protection from cell death.

    Original languageEnglish
    Pages (from-to)45-54
    Number of pages10
    JournalJournal of Biochemical and Molecular Toxicology
    Volume30
    Issue number1
    DOIs
    Publication statusPublished - 2016 Jan 1

    Bibliographical note

    Publisher Copyright:
    © 2016 Wiley Periodicals, Inc.

    Keywords

    • AGS Cells
    • Genipin
    • JNK
    • Nrf2
    • Reactive Oxygen Species

    ASJC Scopus subject areas

    • Biochemistry
    • Molecular Medicine
    • Molecular Biology
    • Toxicology
    • Health, Toxicology and Mutagenesis

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