Chip-based scanometric detection of mercuric ion using DNA-functionalized gold nanoparticles

Jae Seung Lee; Chad A. Mirkin

doi:10.1021/ac801046a

Chip-based scanometric detection of mercuric ion using DNA-functionalized gold nanoparticles

Jae Seung Lee, Chad A. Mirkin

Research output: Contribution to journal › Article › peer-review

208 Citations (Scopus)

Abstract

We have developed a chip-based scanometric method for the detection of mercuric ion (Hg²⁺). This method takes advantage of the cooperative binding and catalytic properties of DNA-functionalized gold nanoparticles and the selective binding of a thymine-thymine mismatch for Hg²⁺. The limit of detection of this assay in buffer and environmentally relevant samples (lake water) is 10 nM (2 ppb) Hg²⁺, which is the U.S. Environmental Protection Agency (EPA) limit of [Hg²⁺] for drinkable water and 1 order of magnitude lower than previous colorimetric assays. This assay is capable of discriminating Hg²⁺ from 15 other environmentally relevant metal ions. The method is attractive for potential point-of-use applications due to its high throughput, convenient readout, and portability.

Original language	English
Pages (from-to)	6805-6808
Number of pages	4
Journal	Analytical chemistry
Volume	80
Issue number	17
DOIs	https://doi.org/10.1021/ac801046a
Publication status	Published - 2008 Sept 1
Externally published	Yes

ASJC Scopus subject areas

Analytical Chemistry

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title = "Chip-based scanometric detection of mercuric ion using DNA-functionalized gold nanoparticles",

abstract = "We have developed a chip-based scanometric method for the detection of mercuric ion (Hg2+). This method takes advantage of the cooperative binding and catalytic properties of DNA-functionalized gold nanoparticles and the selective binding of a thymine-thymine mismatch for Hg2+. The limit of detection of this assay in buffer and environmentally relevant samples (lake water) is 10 nM (2 ppb) Hg2+, which is the U.S. Environmental Protection Agency (EPA) limit of [Hg2+] for drinkable water and 1 order of magnitude lower than previous colorimetric assays. This assay is capable of discriminating Hg2+ from 15 other environmentally relevant metal ions. The method is attractive for potential point-of-use applications due to its high throughput, convenient readout, and portability.",

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AB - We have developed a chip-based scanometric method for the detection of mercuric ion (Hg2+). This method takes advantage of the cooperative binding and catalytic properties of DNA-functionalized gold nanoparticles and the selective binding of a thymine-thymine mismatch for Hg2+. The limit of detection of this assay in buffer and environmentally relevant samples (lake water) is 10 nM (2 ppb) Hg2+, which is the U.S. Environmental Protection Agency (EPA) limit of [Hg2+] for drinkable water and 1 order of magnitude lower than previous colorimetric assays. This assay is capable of discriminating Hg2+ from 15 other environmentally relevant metal ions. The method is attractive for potential point-of-use applications due to its high throughput, convenient readout, and portability.

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Chip-based scanometric detection of mercuric ion using DNA-functionalized gold nanoparticles

Abstract

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