TY - GEN
T1 - Chitosan-alginate microfibers generation on a microfluidic chip and encapsulation of HepG2 cells
AU - Lee, B. R.
AU - Lee, K. H.
AU - Kang, E.
AU - Lee, S. H.
PY - 2011
Y1 - 2011
N2 - The successful encapsulation of human hepatocellular carcinoma (HepG2) cells would assist applications in tissue engineering. In this paper, we have developed a method to produce chitosan-alginate microfibers within a microfluidic chip. Also, by suspending cells in the mixing solution, we fabricated HepG2 cell-laden fibers. The encapsulated HepG2 cells in the chitosan-alginate fibers were more viable than cells encapsulated in pure alginate fibers, suggesting that chitosan provides a better environment for HepG2 cells than alginate. The proposed technique provided an uniform distribution of cells within the fibers, which has been a critical yet previously unmet requirement for tissue engineering.
AB - The successful encapsulation of human hepatocellular carcinoma (HepG2) cells would assist applications in tissue engineering. In this paper, we have developed a method to produce chitosan-alginate microfibers within a microfluidic chip. Also, by suspending cells in the mixing solution, we fabricated HepG2 cell-laden fibers. The encapsulated HepG2 cells in the chitosan-alginate fibers were more viable than cells encapsulated in pure alginate fibers, suggesting that chitosan provides a better environment for HepG2 cells than alginate. The proposed technique provided an uniform distribution of cells within the fibers, which has been a critical yet previously unmet requirement for tissue engineering.
KW - Chitosan-alginate microfiber
KW - Encapsulation
KW - HepG2 cell
KW - Microfluidic chip
KW - Water-soluble chitosan
UR - http://www.scopus.com/inward/record.url?scp=84883784738&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84883784738&partnerID=8YFLogxK
M3 - Conference contribution
AN - SCOPUS:84883784738
SN - 9781618395955
T3 - 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011
SP - 1125
EP - 1127
BT - 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011
T2 - 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011
Y2 - 2 October 2011 through 6 October 2011
ER -