TY - JOUR
T1 - Chromosomal aberrations in neuroblastoma cell lines identified by cross species color banding and chromosome painting
AU - Kim, Gi Jin
AU - Park, Soo Yeun
AU - Kim, Hyun
AU - Chun, Yong Hyuck
AU - Park, Sun Hwa
PY - 2001
Y1 - 2001
N2 - We have studied cytogenetic rearrangements in karyotypes of five neuroblastoma cell lines [SK-N-AS, SK-N-SH, SH-SY5Y, SK-N-MC, SMS-KCNR] by G-banding, cross species color banding (RxFISH), and fluorescence in situ hybridization (FISH) with chromosome painting probes. Each neuroblastoma cell line had unique modal karyotypic characteristics and showed a variable number of numerical and structural clonal cytogenetic aberrations. The number of rearranged chromosomes in SK-N-AS, SK-N-SH, SH-SY5Y, SK-N-MC, and SMS-KCNR was 11, 3, 7, 14 (tetraploid, 20-21), and 6, respectively. The origins of abnormal chromosomes were effectively analyzed by RxFISH and FISH with multiple chromosome painting probes. The chromosomal origin of the homogeneously staining region in SH-SY5Y was identified as coamplification of chromosome bands 2p13 and 2p24 by chromosome microdissection and FISH. The non-random rearrangements of chromosomes were determined on 1p34∼p36, 6q16∼q21, 8q24, 9q34, 11q13∼q23, 16q23∼q24, 17q21, and 22q31. These results may provide useful information for further molecular characterization of neuroblastoma.
AB - We have studied cytogenetic rearrangements in karyotypes of five neuroblastoma cell lines [SK-N-AS, SK-N-SH, SH-SY5Y, SK-N-MC, SMS-KCNR] by G-banding, cross species color banding (RxFISH), and fluorescence in situ hybridization (FISH) with chromosome painting probes. Each neuroblastoma cell line had unique modal karyotypic characteristics and showed a variable number of numerical and structural clonal cytogenetic aberrations. The number of rearranged chromosomes in SK-N-AS, SK-N-SH, SH-SY5Y, SK-N-MC, and SMS-KCNR was 11, 3, 7, 14 (tetraploid, 20-21), and 6, respectively. The origins of abnormal chromosomes were effectively analyzed by RxFISH and FISH with multiple chromosome painting probes. The chromosomal origin of the homogeneously staining region in SH-SY5Y was identified as coamplification of chromosome bands 2p13 and 2p24 by chromosome microdissection and FISH. The non-random rearrangements of chromosomes were determined on 1p34∼p36, 6q16∼q21, 8q24, 9q34, 11q13∼q23, 16q23∼q24, 17q21, and 22q31. These results may provide useful information for further molecular characterization of neuroblastoma.
UR - http://www.scopus.com/inward/record.url?scp=0034909085&partnerID=8YFLogxK
U2 - 10.1016/S0165-4608(01)00418-6
DO - 10.1016/S0165-4608(01)00418-6
M3 - Article
C2 - 11520559
AN - SCOPUS:0034909085
SN - 0165-4608
VL - 129
SP - 10
EP - 16
JO - Cancer Genetics and Cytogenetics
JF - Cancer Genetics and Cytogenetics
IS - 1
ER -
