Chromosomal cephalosporinase in Enterobacter hormaechei as an ancestor of ACT-1 plasmidmediated AmpC β-lactamase

Kyoung Ho Roh; Wonkeun Song; Hae Sun Chung; Yang Soon Lee; Jong Hwa Yum; Ha Na Yi; Jong Sik Chun; Dongeun Yong; Kyungwon Lee; Yunsop Chong

doi:10.1099/jmm.0.032573-0

Chromosomal cephalosporinase in Enterobacter hormaechei as an ancestor of ACT-1 plasmidmediated AmpC β-lactamase

Kyoung Ho Roh, Wonkeun Song, Hae Sun Chung, Yang Soon Lee, Jong Hwa Yum, Ha Na Yi, Jong Sik Chun, Dongeun Yong, Kyungwon Lee, Yunsop Chong

Research output: Contribution to journal › Article › peer-review

10 Citations (Scopus)

Abstract

In this study of the diversity of AmpC β-lactamase in clinical isolates of Enterobacter spp., a strain was found carrying the plasmid-mediated AmpC β-lactamase ACT-1 gene on its chromosome. The strain was identified as Enterobacter hormaechei using phylogenetic analysis of 16S rRNA and hsp60 genes. In addition, the species was confirmed by DNA-DNA hybridization. The genetic environment of the blaACT-1 gene was characterized, including the ampR and ampG genes, using a two-step PCR. The amino acid sequences of AmpR at serine 35, arginine 86, glycine 102, aspartic acid 135 and tyrosine 264 were conserved. Measurement of the transcription level of the blaACT-1 gene using real-time quantitative PCR showed that it increased 1.98-fold following cefoxitin induction. These results suggest that the plasmid-mediated blaACT-1 gene originated from the chromosome of E. hormaechei.

Original language	English
Pages (from-to)	94-100
Number of pages	7
Journal	Journal of Medical Microbiology
Volume	61
Issue number	1
DOIs	https://doi.org/10.1099/jmm.0.032573-0
Publication status	Published - 2012 Jan
Externally published	Yes

ASJC Scopus subject areas

Microbiology
Microbiology (medical)

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title = "Chromosomal cephalosporinase in Enterobacter hormaechei as an ancestor of ACT-1 plasmidmediated AmpC β-lactamase",

abstract = "In this study of the diversity of AmpC β-lactamase in clinical isolates of Enterobacter spp., a strain was found carrying the plasmid-mediated AmpC β-lactamase ACT-1 gene on its chromosome. The strain was identified as Enterobacter hormaechei using phylogenetic analysis of 16S rRNA and hsp60 genes. In addition, the species was confirmed by DNA-DNA hybridization. The genetic environment of the blaACT-1 gene was characterized, including the ampR and ampG genes, using a two-step PCR. The amino acid sequences of AmpR at serine 35, arginine 86, glycine 102, aspartic acid 135 and tyrosine 264 were conserved. Measurement of the transcription level of the blaACT-1 gene using real-time quantitative PCR showed that it increased 1.98-fold following cefoxitin induction. These results suggest that the plasmid-mediated blaACT-1 gene originated from the chromosome of E. hormaechei.",

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year = "2012",

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doi = "10.1099/jmm.0.032573-0",

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AU - Roh, Kyoung Ho

AU - Song, Wonkeun

AU - Chung, Hae Sun

AU - Lee, Yang Soon

AU - Yum, Jong Hwa

AU - Yi, Ha Na

AU - Chun, Jong Sik

AU - Yong, Dongeun

AU - Lee, Kyungwon

AU - Chong, Yunsop

PY - 2012/1

Y1 - 2012/1

N2 - In this study of the diversity of AmpC β-lactamase in clinical isolates of Enterobacter spp., a strain was found carrying the plasmid-mediated AmpC β-lactamase ACT-1 gene on its chromosome. The strain was identified as Enterobacter hormaechei using phylogenetic analysis of 16S rRNA and hsp60 genes. In addition, the species was confirmed by DNA-DNA hybridization. The genetic environment of the blaACT-1 gene was characterized, including the ampR and ampG genes, using a two-step PCR. The amino acid sequences of AmpR at serine 35, arginine 86, glycine 102, aspartic acid 135 and tyrosine 264 were conserved. Measurement of the transcription level of the blaACT-1 gene using real-time quantitative PCR showed that it increased 1.98-fold following cefoxitin induction. These results suggest that the plasmid-mediated blaACT-1 gene originated from the chromosome of E. hormaechei.

AB - In this study of the diversity of AmpC β-lactamase in clinical isolates of Enterobacter spp., a strain was found carrying the plasmid-mediated AmpC β-lactamase ACT-1 gene on its chromosome. The strain was identified as Enterobacter hormaechei using phylogenetic analysis of 16S rRNA and hsp60 genes. In addition, the species was confirmed by DNA-DNA hybridization. The genetic environment of the blaACT-1 gene was characterized, including the ampR and ampG genes, using a two-step PCR. The amino acid sequences of AmpR at serine 35, arginine 86, glycine 102, aspartic acid 135 and tyrosine 264 were conserved. Measurement of the transcription level of the blaACT-1 gene using real-time quantitative PCR showed that it increased 1.98-fold following cefoxitin induction. These results suggest that the plasmid-mediated blaACT-1 gene originated from the chromosome of E. hormaechei.

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Chromosomal cephalosporinase in Enterobacter hormaechei as an ancestor of ACT-1 plasmidmediated AmpC β-lactamase

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