Chryseobacterium faecale sp. nov., isolated from camel feces

Yongjun Son, Jihyeon Min, Woojun Park

Research output: Contribution to journalArticlepeer-review

5 Citations (Scopus)

Abstract

Strain F4T (=KACC 22401T=JCM 34836T), a novel Gram-stain-negative, aerobic, non-spore-forming, non-motile and rod-shaped bacterium, was isolated from camel (Camelus bactrianus) faeces. The newly identified bacterial strain F4T was grown in Rea-soner's 2A medium [0–2% (w/v) NaCl (optimum, 0%), pH 7.0–8.0 (optimum, pH 7.0), and 18–40 °C (optimum, 30 °C)]. Phylogenetic analysis based on 16S rRNA gene sequencing confirmed that strain F4T belonged to the genus Chryseobacterium, with its closest neighbours being Chryseobacterium haifense DSM 19056T (98.0%), Chryseobacterium anthropi CCUG 52764T (97.3%), Chryseobacterium montana WG4T (95.7%) and Chryseobacterium koreensis Chj70T (94.7%). Complete genome sequence of strain F4T was obtained using a hybrid assembly pipeline integrating sequences obtained using both the Oxford Nanopore and Illumina platforms. Genomic comparisons of strain F4T with type species in the genus Chryseobacterium were conducted using digital DNA–DNA hybridization, average nucleotide identity and average amino acid identity, resulting in values of ≤20.5, ≤77.9 and ≤80.8 %, respectively. The genomic DNA G+C content of type strain F4T was 39.7 mol%. The major fatty acids of the strain F4T were anteiso-C15:0 and iso-C18:3, and MK-6 was its major respiratory quinone. Moreover, the major polar lipid of strain F4T was phosphatidylethanolamine. The genome of strain F4T harbours only one antibiotic resistance gene (blaCME-1) encoding a β-lactamase, which attributes β-lactam antibiotic resistance. Based on the results of our chemotaxonomic, genotypic and phe-notype analyses, strain F4T is identified as a novel species of the genus Chryseobacterium, for which the name Chryseobacterium faecale sp. nov. is proposed.

Original languageEnglish
Article number005405
JournalInternational Journal of Systematic and Evolutionary Microbiology
Volume72
Issue number5
DOIs
Publication statusPublished - 2022

Bibliographical note

Funding Information:
This work was supported by a grant from the National Institute of Biological Resources (NIBR), funded by the Ministry of Environment (MOE) of the Republic of Korea (NIBR202002108).

Publisher Copyright:
© 2022 The Authors.

Keywords

  • Chryseobacterium
  • Flavobacteriia
  • Weeksellaceae
  • pathogen
  • taxonomy
  • type Ⅸ secretion system

ASJC Scopus subject areas

  • Microbiology
  • Ecology, Evolution, Behavior and Systematics

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