Cigarette smoke extract inhibits cell migration and contraction via the reactive oxygen species/adenosine monophosphate–activated protein kinase pathway in nasal fibroblasts

Jae Min Shin; Joo Hoo Park; Hyun Woo Yang; Heung Man Lee; Il Ho Park

doi:10.1002/alr.22479

Cigarette smoke extract inhibits cell migration and contraction via the reactive oxygen species/adenosine monophosphate–activated protein kinase pathway in nasal fibroblasts

Jae Min Shin, Joo Hoo Park, Hyun Woo Yang, Heung Man Lee, Il Ho Park

Research output: Contribution to journal › Article › peer-review

10 Citations (Scopus)

Abstract

Background: Fibroblast migration plays a significant role in wound healing after endoscopic sinonasal surgery. Cigarette smoke extract (CSE) is a potent inhibitor of fibroblast functions including cell proliferation and migration. The purpose of the study was to determine the influence of CSE on migration and collagen gel contraction in nasal fibroblasts and investigate its underlying mechanisms. Methods: Fibroblast migration was evaluated using wound healing assay and transwell migration assay. Contractile activity was assessed by collagen gel contraction assay. Reactive oxygen species (ROS) were quantified by 2′,7′-dichlorofluorescein diacetate. Fibroblasts were treated with CSE and N-acetylcysteine (NAC), metformin, compound C, or transfected with small interfering RNA (siRNA) to suppress adenosine monophosphate–activated protein kinase (AMPK) expression. AMPK activation was determined by Western blot. Results: CSE and metformin were found to significantly reduce the migration and collagen gel contraction activity of nasal fibroblasts. Conversely, pretreatment with NAC and compound C significantly enhanced the migration and collagen gel contraction activity of fibroblasts. ROS production and AMPK phosphorylation were found to be significantly induced by CSE treatment, whereas the activity was inhibited on treatment with NAC, metformin, compound C, or AMPK siRNA. Silencing of AMPK expression was found to significantly reverse the suppressive effect of CSE in nasal fibroblasts. Conclusion: CSE has an inhibitory effect on cell migration and collagen gel contraction activity via the ROS/AMPK signaling pathway in nasal fibroblasts.

Original language	English
Pages (from-to)	356-363
Number of pages	8
Journal	International Forum of Allergy and Rhinology
Volume	10
Issue number	3
DOIs	https://doi.org/10.1002/alr.22479
Publication status	Published - 2020 Mar 1

Bibliographical note

Funding Information:
sources for the study: Korean Health Technology R&D Project, Ministry of Health & Welfare, Republic of Korea (HI15C1512) and Grant of the Establish R&D Platform Project, Korea University Medical Center and Korea University Guro hospital (O1903851).

Publisher Copyright:
© 2019 ARS-AAOA, LLC

Keywords

cigarette smoke extract
fibroblast
migration
reactive oxygen species
wound healing

ASJC Scopus subject areas

Immunology and Allergy
Otorhinolaryngology

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10.1002/alr.22479

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Cigarette smoke extract inhibits cell migration and contraction via the reactive oxygen species/adenosine monophosphate–activated protein kinase pathway in nasal fibroblasts. / Shin, Jae Min; Park, Joo Hoo; Yang, Hyun Woo et al.
In: International Forum of Allergy and Rhinology, Vol. 10, No. 3, 01.03.2020, p. 356-363.

Research output: Contribution to journal › Article › peer-review

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title = "Cigarette smoke extract inhibits cell migration and contraction via the reactive oxygen species/adenosine monophosphate–activated protein kinase pathway in nasal fibroblasts",

abstract = "Background: Fibroblast migration plays a significant role in wound healing after endoscopic sinonasal surgery. Cigarette smoke extract (CSE) is a potent inhibitor of fibroblast functions including cell proliferation and migration. The purpose of the study was to determine the influence of CSE on migration and collagen gel contraction in nasal fibroblasts and investigate its underlying mechanisms. Methods: Fibroblast migration was evaluated using wound healing assay and transwell migration assay. Contractile activity was assessed by collagen gel contraction assay. Reactive oxygen species (ROS) were quantified by 2′,7′-dichlorofluorescein diacetate. Fibroblasts were treated with CSE and N-acetylcysteine (NAC), metformin, compound C, or transfected with small interfering RNA (siRNA) to suppress adenosine monophosphate–activated protein kinase (AMPK) expression. AMPK activation was determined by Western blot. Results: CSE and metformin were found to significantly reduce the migration and collagen gel contraction activity of nasal fibroblasts. Conversely, pretreatment with NAC and compound C significantly enhanced the migration and collagen gel contraction activity of fibroblasts. ROS production and AMPK phosphorylation were found to be significantly induced by CSE treatment, whereas the activity was inhibited on treatment with NAC, metformin, compound C, or AMPK siRNA. Silencing of AMPK expression was found to significantly reverse the suppressive effect of CSE in nasal fibroblasts. Conclusion: CSE has an inhibitory effect on cell migration and collagen gel contraction activity via the ROS/AMPK signaling pathway in nasal fibroblasts.",

keywords = "cigarette smoke extract, fibroblast, migration, reactive oxygen species, wound healing",

author = "Shin, {Jae Min} and Park, {Joo Hoo} and Yang, {Hyun Woo} and Lee, {Heung Man} and Park, {Il Ho}",

note = "Funding Information: sources for the study: Korean Health Technology R&D Project, Ministry of Health & Welfare, Republic of Korea (HI15C1512) and Grant of the Establish R&D Platform Project, Korea University Medical Center and Korea University Guro hospital (O1903851). Publisher Copyright: {\textcopyright} 2019 ARS-AAOA, LLC",

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AU - Lee, Heung Man

AU - Park, Il Ho

N1 - Funding Information: sources for the study: Korean Health Technology R&D Project, Ministry of Health & Welfare, Republic of Korea (HI15C1512) and Grant of the Establish R&D Platform Project, Korea University Medical Center and Korea University Guro hospital (O1903851). Publisher Copyright: © 2019 ARS-AAOA, LLC

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N2 - Background: Fibroblast migration plays a significant role in wound healing after endoscopic sinonasal surgery. Cigarette smoke extract (CSE) is a potent inhibitor of fibroblast functions including cell proliferation and migration. The purpose of the study was to determine the influence of CSE on migration and collagen gel contraction in nasal fibroblasts and investigate its underlying mechanisms. Methods: Fibroblast migration was evaluated using wound healing assay and transwell migration assay. Contractile activity was assessed by collagen gel contraction assay. Reactive oxygen species (ROS) were quantified by 2′,7′-dichlorofluorescein diacetate. Fibroblasts were treated with CSE and N-acetylcysteine (NAC), metformin, compound C, or transfected with small interfering RNA (siRNA) to suppress adenosine monophosphate–activated protein kinase (AMPK) expression. AMPK activation was determined by Western blot. Results: CSE and metformin were found to significantly reduce the migration and collagen gel contraction activity of nasal fibroblasts. Conversely, pretreatment with NAC and compound C significantly enhanced the migration and collagen gel contraction activity of fibroblasts. ROS production and AMPK phosphorylation were found to be significantly induced by CSE treatment, whereas the activity was inhibited on treatment with NAC, metformin, compound C, or AMPK siRNA. Silencing of AMPK expression was found to significantly reverse the suppressive effect of CSE in nasal fibroblasts. Conclusion: CSE has an inhibitory effect on cell migration and collagen gel contraction activity via the ROS/AMPK signaling pathway in nasal fibroblasts.

AB - Background: Fibroblast migration plays a significant role in wound healing after endoscopic sinonasal surgery. Cigarette smoke extract (CSE) is a potent inhibitor of fibroblast functions including cell proliferation and migration. The purpose of the study was to determine the influence of CSE on migration and collagen gel contraction in nasal fibroblasts and investigate its underlying mechanisms. Methods: Fibroblast migration was evaluated using wound healing assay and transwell migration assay. Contractile activity was assessed by collagen gel contraction assay. Reactive oxygen species (ROS) were quantified by 2′,7′-dichlorofluorescein diacetate. Fibroblasts were treated with CSE and N-acetylcysteine (NAC), metformin, compound C, or transfected with small interfering RNA (siRNA) to suppress adenosine monophosphate–activated protein kinase (AMPK) expression. AMPK activation was determined by Western blot. Results: CSE and metformin were found to significantly reduce the migration and collagen gel contraction activity of nasal fibroblasts. Conversely, pretreatment with NAC and compound C significantly enhanced the migration and collagen gel contraction activity of fibroblasts. ROS production and AMPK phosphorylation were found to be significantly induced by CSE treatment, whereas the activity was inhibited on treatment with NAC, metformin, compound C, or AMPK siRNA. Silencing of AMPK expression was found to significantly reverse the suppressive effect of CSE in nasal fibroblasts. Conclusion: CSE has an inhibitory effect on cell migration and collagen gel contraction activity via the ROS/AMPK signaling pathway in nasal fibroblasts.

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Cigarette smoke extract inhibits cell migration and contraction via the reactive oxygen species/adenosine monophosphate–activated protein kinase pathway in nasal fibroblasts

Abstract

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Keywords

ASJC Scopus subject areas

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