CIIA negatively regulates neuronal cell death induced by oxygen–glucose deprivation and reoxygenation

Sang Gil Hwang, Jaekyung Shim, Eui Ju Choi

Research output: Contribution to journalArticlepeer-review

5 Citations (Scopus)

Abstract

Brain ischemia causes neuronal injury leading to stroke and other related brain diseases. However, the precise mechanism of the ischemia-induced neuronal death remains unclear yet. In this study, we showed that CIIA suppressed neuronal cell death induced by oxygen and glucose deprivation followed by reoxygenation (OGD/R), which mimics ischemia and reperfusion in vivo, in neuroblastoma cell lines as well as primary cortical neurons. Furthermore, CIIA inhibited the OGD/R-induced stimulation of apoptosis signal-regulating kinase 1 (ASK1) and its downstream kinases including c-Jun amino-terminal kinase and p38 kinase, concomitantly blocking ASK1 homo-oligomerization and the binding between ASK1 and TRAF2. CIIA also repressed the OGD/R-induced activation of caspase-3 in neuronal cells. Taken together, our results suggest that CIIA attenuates neurotoxicity caused by OGD/R through inhibiting ASK1-dependent signaling events.

Original languageEnglish
Pages (from-to)139-146
Number of pages8
JournalMolecular and Cellular Biochemistry
Volume397
Issue number1-2
DOIs
Publication statusPublished - 2014 Oct 29

Bibliographical note

Funding Information:
Acknowledgments We thank Drs. H. Ichijo for ASK1 cDNA, J. Han for MKK6 cDNA. This work was supported by the National Research Foundation Grant (2006-0093855) and a NRF Grant (2011-0030141) through the National Research Foundation of Korea funded by the Ministry of Science, ICT and Future Planning (MEST) of the Korea, and by a Korea University Grant (E.-J.C.).

Publisher Copyright:
© 2014, Springer Science+Business Media New York.

Keywords

  • ASK1
  • CIIA
  • Oxygen and glucose deprivation
  • Reoxygenation

ASJC Scopus subject areas

  • Molecular Biology
  • Clinical Biochemistry
  • Cell Biology

Fingerprint

Dive into the research topics of 'CIIA negatively regulates neuronal cell death induced by oxygen–glucose deprivation and reoxygenation'. Together they form a unique fingerprint.

Cite this