Circular permutation of the DNA genome of temperate bacteriophage φFC1 from Enterococcus faecalis KBL703

Y. W. Kim, S. H. Jang, B. S. Hong, W. J. Lim, C. W. Kim, H. C. Sung, I. Chang

    Research output: Contribution to journalArticlepeer-review

    Abstract

    The physical map of bacteriophage φFC1 DNA was constructed with the restriction endonucleases SaII, BarnHI, EcoRI, XbaI, and AvaI. The 40.5-kb DNA restriction map is shown to be circularly permuted representing the headful packaging mechanism of the phage. The DNA restriction fragments containing the packaging initiation site (pac) was localized on the restriction map and the nucleotide sequences of the region were analyzed. Four open reading frames (ORFs), following one another with the same orientation, were found at the region. The 2nd ORF (ORF-ts) has significant amino acid sequence homologies to the previously known terminase small subunits of other bacteriophages. The putative terminase small subunit gene has a presumptive NTP-hydrolysis motif and a helix-turn-helix motif. The cleavage site for the first round of packaging was found to be located at the coding sequence of the putative terminase small subunit gene. The fourth ORF, even if partially sequenced, has a good amino acid sequence homology to the portal vertex proteins of other bacteriophages representing the evolutionarily conserved arrangements of genes near the pac site of this bacteriophage, φFC1.

    Original languageEnglish
    Pages (from-to)457-463
    Number of pages7
    JournalJournal of microbiology and biotechnology
    Volume9
    Issue number4
    Publication statusPublished - 1999

    Keywords

    • Bacteriophage φFC1
    • Pac site
    • Restriction map
    • Terminase small subunit

    ASJC Scopus subject areas

    • Biotechnology
    • Applied Microbiology and Biotechnology

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