cis-prenyltransferase 3 and α/β-hydrolase are new determinants of dolichol accumulation in Arabidopsis

Katarzyna Gawarecka; Joanna Siwinska; Jaroslaw Poznanski; Agnieszka Onysk; Przemyslaw Surowiecki; Karolina Sztompka; Liliana Surmacz; Ji Hoon Ahn; Arthur Korte; Ewa Swiezewska; Anna Ihnatowicz

doi:10.1111/pce.14223

cis-prenyltransferase 3 and α/β-hydrolase are new determinants of dolichol accumulation in Arabidopsis

Katarzyna Gawarecka, Joanna Siwinska, Jaroslaw Poznanski, Agnieszka Onysk, Przemyslaw Surowiecki, Karolina Sztompka, Liliana Surmacz, Ji Hoon Ahn, Arthur Korte, Ewa Swiezewska, Anna Ihnatowicz

Department of Life Sciences

Research output: Contribution to journal › Article › peer-review

4 Citations (Scopus)

Abstract

Dolichols (Dols), ubiquitous components of living organisms, are indispensable for cell survival. In plants, as well as other eukaryotes, Dols are crucial for post-translational protein glycosylation, aberration of which leads to fatal metabolic disorders in humans and male sterility in plants. Until now, the mechanisms underlying Dol accumulation remain elusive. In this study, we have analysed the natural variation of the accumulation of Dols and six other isoprenoids among more than 120 Arabidopsis thaliana accessions. Subsequently, by combining QTL and GWAS approaches, we have identified several candidate genes involved in the accumulation of Dols, polyprenols, plastoquinone and phytosterols. The role of two genes implicated in the accumulation of major Dols in Arabidopsis—the AT2G17570 gene encoding a long searched for cis-prenyltransferase (CPT3) and the AT1G52460 gene encoding an α/β-hydrolase—is experimentally confirmed. These data will help to generate Dol-enriched plants which might serve as a remedy for Dol-deficiency in humans.

Original language	English
Pages (from-to)	479-495
Number of pages	17
Journal	Plant, Cell and Environment
Volume	45
Issue number	2
DOIs	https://doi.org/10.1111/pce.14223
Publication status	Published - 2022 Feb

Bibliographical note

Funding Information:
This study was supported by grants from the National Science Centre of Poland [UMO‐2014/15/N/NZ3/04316] (Katarzyna Gawarecka), [UMO‐2018/29/B/NZ3/01033] (Ewa Swiezewska), and [UMO‐2014/15/B/NZ2/01073] (Anna Ihnatowicz), and the National Research Foundation (NRF) of Korea [NRF—2017R1A2B3009624] (Ji Hoon Ahn). We would like to express our gratitude to Professor Maarten Koornneef for providing the AI‐RILs seeds used in this study. We also would like to thank Dr. Agata Lipko for initial characterisation of mutant lines. Dr. Marta Hoffman‐Sommer is kindly acknowledged for help with preparation of the manuscript.

Publisher Copyright:
© 2021 The Authors. Plant, Cell & Environment published by John Wiley & Sons Ltd.

ASJC Scopus subject areas

Physiology
Plant Science

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10.1111/pce.14223

Cite this

@article{c970964c2e184f72a98bd2da0792da94,

title = "cis-prenyltransferase 3 and α/β-hydrolase are new determinants of dolichol accumulation in Arabidopsis",

abstract = "Dolichols (Dols), ubiquitous components of living organisms, are indispensable for cell survival. In plants, as well as other eukaryotes, Dols are crucial for post-translational protein glycosylation, aberration of which leads to fatal metabolic disorders in humans and male sterility in plants. Until now, the mechanisms underlying Dol accumulation remain elusive. In this study, we have analysed the natural variation of the accumulation of Dols and six other isoprenoids among more than 120 Arabidopsis thaliana accessions. Subsequently, by combining QTL and GWAS approaches, we have identified several candidate genes involved in the accumulation of Dols, polyprenols, plastoquinone and phytosterols. The role of two genes implicated in the accumulation of major Dols in Arabidopsis—the AT2G17570 gene encoding a long searched for cis-prenyltransferase (CPT3) and the AT1G52460 gene encoding an α/β-hydrolase—is experimentally confirmed. These data will help to generate Dol-enriched plants which might serve as a remedy for Dol-deficiency in humans.",

author = "Katarzyna Gawarecka and Joanna Siwinska and Jaroslaw Poznanski and Agnieszka Onysk and Przemyslaw Surowiecki and Karolina Sztompka and Liliana Surmacz and Ahn, {Ji Hoon} and Arthur Korte and Ewa Swiezewska and Anna Ihnatowicz",

note = "Funding Information: This study was supported by grants from the National Science Centre of Poland [UMO‐2014/15/N/NZ3/04316] (Katarzyna Gawarecka), [UMO‐2018/29/B/NZ3/01033] (Ewa Swiezewska), and [UMO‐2014/15/B/NZ2/01073] (Anna Ihnatowicz), and the National Research Foundation (NRF) of Korea [NRF—2017R1A2B3009624] (Ji Hoon Ahn). We would like to express our gratitude to Professor Maarten Koornneef for providing the AI‐RILs seeds used in this study. We also would like to thank Dr. Agata Lipko for initial characterisation of mutant lines. Dr. Marta Hoffman‐Sommer is kindly acknowledged for help with preparation of the manuscript. Publisher Copyright: {\textcopyright} 2021 The Authors. Plant, Cell & Environment published by John Wiley & Sons Ltd.",

year = "2022",

month = feb,

doi = "10.1111/pce.14223",

language = "English",

volume = "45",

pages = "479--495",

journal = "Plant, Cell and Environment",

issn = "0140-7791",

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T1 - cis-prenyltransferase 3 and α/β-hydrolase are new determinants of dolichol accumulation in Arabidopsis

AU - Gawarecka, Katarzyna

AU - Siwinska, Joanna

AU - Poznanski, Jaroslaw

AU - Onysk, Agnieszka

AU - Surowiecki, Przemyslaw

AU - Sztompka, Karolina

AU - Surmacz, Liliana

AU - Ahn, Ji Hoon

AU - Korte, Arthur

AU - Swiezewska, Ewa

AU - Ihnatowicz, Anna

N1 - Funding Information: This study was supported by grants from the National Science Centre of Poland [UMO‐2014/15/N/NZ3/04316] (Katarzyna Gawarecka), [UMO‐2018/29/B/NZ3/01033] (Ewa Swiezewska), and [UMO‐2014/15/B/NZ2/01073] (Anna Ihnatowicz), and the National Research Foundation (NRF) of Korea [NRF—2017R1A2B3009624] (Ji Hoon Ahn). We would like to express our gratitude to Professor Maarten Koornneef for providing the AI‐RILs seeds used in this study. We also would like to thank Dr. Agata Lipko for initial characterisation of mutant lines. Dr. Marta Hoffman‐Sommer is kindly acknowledged for help with preparation of the manuscript. Publisher Copyright: © 2021 The Authors. Plant, Cell & Environment published by John Wiley & Sons Ltd.

PY - 2022/2

Y1 - 2022/2

N2 - Dolichols (Dols), ubiquitous components of living organisms, are indispensable for cell survival. In plants, as well as other eukaryotes, Dols are crucial for post-translational protein glycosylation, aberration of which leads to fatal metabolic disorders in humans and male sterility in plants. Until now, the mechanisms underlying Dol accumulation remain elusive. In this study, we have analysed the natural variation of the accumulation of Dols and six other isoprenoids among more than 120 Arabidopsis thaliana accessions. Subsequently, by combining QTL and GWAS approaches, we have identified several candidate genes involved in the accumulation of Dols, polyprenols, plastoquinone and phytosterols. The role of two genes implicated in the accumulation of major Dols in Arabidopsis—the AT2G17570 gene encoding a long searched for cis-prenyltransferase (CPT3) and the AT1G52460 gene encoding an α/β-hydrolase—is experimentally confirmed. These data will help to generate Dol-enriched plants which might serve as a remedy for Dol-deficiency in humans.

AB - Dolichols (Dols), ubiquitous components of living organisms, are indispensable for cell survival. In plants, as well as other eukaryotes, Dols are crucial for post-translational protein glycosylation, aberration of which leads to fatal metabolic disorders in humans and male sterility in plants. Until now, the mechanisms underlying Dol accumulation remain elusive. In this study, we have analysed the natural variation of the accumulation of Dols and six other isoprenoids among more than 120 Arabidopsis thaliana accessions. Subsequently, by combining QTL and GWAS approaches, we have identified several candidate genes involved in the accumulation of Dols, polyprenols, plastoquinone and phytosterols. The role of two genes implicated in the accumulation of major Dols in Arabidopsis—the AT2G17570 gene encoding a long searched for cis-prenyltransferase (CPT3) and the AT1G52460 gene encoding an α/β-hydrolase—is experimentally confirmed. These data will help to generate Dol-enriched plants which might serve as a remedy for Dol-deficiency in humans.

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DO - 10.1111/pce.14223

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SN - 0140-7791

VL - 45

SP - 479

EP - 495

JO - Plant, Cell and Environment

JF - Plant, Cell and Environment

IS - 2

ER -

cis-prenyltransferase 3 and α/β-hydrolase are new determinants of dolichol accumulation in Arabidopsis

Abstract

Bibliographical note

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