TY - JOUR
T1 - cis-prenyltransferase 3 and α/β-hydrolase are new determinants of dolichol accumulation in Arabidopsis
AU - Gawarecka, Katarzyna
AU - Siwinska, Joanna
AU - Poznanski, Jaroslaw
AU - Onysk, Agnieszka
AU - Surowiecki, Przemyslaw
AU - Sztompka, Karolina
AU - Surmacz, Liliana
AU - Ahn, Ji Hoon
AU - Korte, Arthur
AU - Swiezewska, Ewa
AU - Ihnatowicz, Anna
N1 - Funding Information:
This study was supported by grants from the National Science Centre of Poland [UMO‐2014/15/N/NZ3/04316] (Katarzyna Gawarecka), [UMO‐2018/29/B/NZ3/01033] (Ewa Swiezewska), and [UMO‐2014/15/B/NZ2/01073] (Anna Ihnatowicz), and the National Research Foundation (NRF) of Korea [NRF—2017R1A2B3009624] (Ji Hoon Ahn). We would like to express our gratitude to Professor Maarten Koornneef for providing the AI‐RILs seeds used in this study. We also would like to thank Dr. Agata Lipko for initial characterisation of mutant lines. Dr. Marta Hoffman‐Sommer is kindly acknowledged for help with preparation of the manuscript.
Publisher Copyright:
© 2021 The Authors. Plant, Cell & Environment published by John Wiley & Sons Ltd.
PY - 2022/2
Y1 - 2022/2
N2 - Dolichols (Dols), ubiquitous components of living organisms, are indispensable for cell survival. In plants, as well as other eukaryotes, Dols are crucial for post-translational protein glycosylation, aberration of which leads to fatal metabolic disorders in humans and male sterility in plants. Until now, the mechanisms underlying Dol accumulation remain elusive. In this study, we have analysed the natural variation of the accumulation of Dols and six other isoprenoids among more than 120 Arabidopsis thaliana accessions. Subsequently, by combining QTL and GWAS approaches, we have identified several candidate genes involved in the accumulation of Dols, polyprenols, plastoquinone and phytosterols. The role of two genes implicated in the accumulation of major Dols in Arabidopsis—the AT2G17570 gene encoding a long searched for cis-prenyltransferase (CPT3) and the AT1G52460 gene encoding an α/β-hydrolase—is experimentally confirmed. These data will help to generate Dol-enriched plants which might serve as a remedy for Dol-deficiency in humans.
AB - Dolichols (Dols), ubiquitous components of living organisms, are indispensable for cell survival. In plants, as well as other eukaryotes, Dols are crucial for post-translational protein glycosylation, aberration of which leads to fatal metabolic disorders in humans and male sterility in plants. Until now, the mechanisms underlying Dol accumulation remain elusive. In this study, we have analysed the natural variation of the accumulation of Dols and six other isoprenoids among more than 120 Arabidopsis thaliana accessions. Subsequently, by combining QTL and GWAS approaches, we have identified several candidate genes involved in the accumulation of Dols, polyprenols, plastoquinone and phytosterols. The role of two genes implicated in the accumulation of major Dols in Arabidopsis—the AT2G17570 gene encoding a long searched for cis-prenyltransferase (CPT3) and the AT1G52460 gene encoding an α/β-hydrolase—is experimentally confirmed. These data will help to generate Dol-enriched plants which might serve as a remedy for Dol-deficiency in humans.
UR - http://www.scopus.com/inward/record.url?scp=85120031860&partnerID=8YFLogxK
U2 - 10.1111/pce.14223
DO - 10.1111/pce.14223
M3 - Article
C2 - 34778961
AN - SCOPUS:85120031860
SN - 0140-7791
VL - 45
SP - 479
EP - 495
JO - Plant, Cell and Environment
JF - Plant, Cell and Environment
IS - 2
ER -