TY - JOUR
T1 - Clerodane Diterpenoids Identified fromPolyalthia longifoliaShowing Antifungal Activity against Plant Pathogens
AU - Nguyen, Minh Van
AU - Han, Jae Woo
AU - Le Dang, Quang
AU - Ryu, Seung Mok
AU - Lee, Dongho
AU - Kim, Hun
AU - Choi, Gyung Ja
N1 - Funding Information:
This study was carried out with the support of the Cooperative Research Program for Agriculture Science and Technology Development (Project no. PJ01481604), the Rural Development Administration, Republic of Korea, and the Korea Institute of Planning and Evaluation for Technology in Food, Agriculture, Forestry and Fisheries (IPET) through Crop Viruses and Pests Response Industry Technology Development Program, funded by Ministry of Agriculture, Food and Rural Affairs (MAFRA) (no. 321101-03).
Publisher Copyright:
© 2021 American Chemical Society
PY - 2021/9/15
Y1 - 2021/9/15
N2 - In the search for new natural resources showing plant disease control effects, we found that the methanol extract ofPolyalthia longifoliasuppressed fungal disease development in plants. To identify the bioactive substances, the methanol extract ofP. longifoliawas extracted by organic solvents, and consequently, four new 2-oxo-clerodane diterpenes (1-4), a new 4(3 → 2)-abeo-clerodane diterpene (5), together with ten known compounds (6-16) were isolated and identified from the extracts. Of the new compounds, compound2showed a broad spectrum of antifungal activity with moderated minimum inhibitory concentration (MIC) values in a range of 50-100 μg/mL against tested fungal pathogens. Considering with the known compounds, compound6showed the most potent antifungal activity with an MIC value in the range of 6.3-12.5 μg/mL. When compound6was evaluated for anin vivoantifungal activity against rice blast, tomato late blight, and pepper anthracnose, compound6reduced the plant disease by at least 60% compared to the untreated control at concentrations of 250 and 500 μg/mL. Together, our results suggested that the methanol extract of twigs and leaves ofP. longifoliaand its major compound6could be used as a source for the development of eco-friendly plant protection agents.
AB - In the search for new natural resources showing plant disease control effects, we found that the methanol extract ofPolyalthia longifoliasuppressed fungal disease development in plants. To identify the bioactive substances, the methanol extract ofP. longifoliawas extracted by organic solvents, and consequently, four new 2-oxo-clerodane diterpenes (1-4), a new 4(3 → 2)-abeo-clerodane diterpene (5), together with ten known compounds (6-16) were isolated and identified from the extracts. Of the new compounds, compound2showed a broad spectrum of antifungal activity with moderated minimum inhibitory concentration (MIC) values in a range of 50-100 μg/mL against tested fungal pathogens. Considering with the known compounds, compound6showed the most potent antifungal activity with an MIC value in the range of 6.3-12.5 μg/mL. When compound6was evaluated for anin vivoantifungal activity against rice blast, tomato late blight, and pepper anthracnose, compound6reduced the plant disease by at least 60% compared to the untreated control at concentrations of 250 and 500 μg/mL. Together, our results suggested that the methanol extract of twigs and leaves ofP. longifoliaand its major compound6could be used as a source for the development of eco-friendly plant protection agents.
KW - Polyalthia longifolia
KW - antifungal
KW - clerodane diterpenes
KW - plant disease control
UR - http://www.scopus.com/inward/record.url?scp=85115011420&partnerID=8YFLogxK
U2 - 10.1021/acs.jafc.1c02200
DO - 10.1021/acs.jafc.1c02200
M3 - Article
C2 - 34469148
AN - SCOPUS:85115011420
SN - 0021-8561
VL - 69
SP - 10527
EP - 10535
JO - Journal of agricultural and food chemistry
JF - Journal of agricultural and food chemistry
IS - 36
ER -