A combination of a radiotherapeutic regimen with telomerase inhibition is valuable when tumor cells are to be sensitized to radiation. Here, we describe cell clones unresponsive to radiosensitization after telomere shortening. After extensive division of individual transformed clones of mTERC-/- cells, about 22% of clones were unresponsive to radiosensitization even though telomerase action was inhibited. The telomere lengths of unsensitized mTERC-/- clones were reduced, as were those of most sensitized clones. However, the unsensitized clones did not exhibit chromosomal end-to-end fusion to the extent noted in all sensitized clones. Thus, a defense mechanism preventing telomere erosion is operative even when telomeres become shorter under conditions of telomerase deficiency, and results in unresponsiveness to the radiosensitization generally mediated by telomere shortening.
|Number of pages||5|
|Journal||Biochemical and biophysical research communications|
|Publication status||Published - 2010 Nov 12|
Bibliographical noteFunding Information:
This work was supported by a grant from the National Nuclear R & D program and BAERI, the Korean Ministry of Science and Technology . M.H.C. was supported by a grant from the Korea Research Foundation , funded by the Korean Government (Grant No. KRF-2006-311-E00507 ).
- Chromosomal end-to-end fusion
- Telomere length
ASJC Scopus subject areas
- Molecular Biology
- Cell Biology