Cloning and characterization of microRNAs from porcine skeletal muscle and adipose tissue

Ik Sang Cho; Jung Kim; Hye Youn Seo; Do Hwan Lim; Jae Sang Hong; Young Hee Park; Dae Cheul Park; Ki Chang Hong; Kwang Youn Whang; Young Sik Lee

doi:10.1007/s11033-010-0005-6

Cloning and characterization of microRNAs from porcine skeletal muscle and adipose tissue

Ik Sang Cho, Jung Kim, Hye Youn Seo, Do Hwan Lim, Jae Sang Hong, Young Hee Park, Dae Cheul Park, Ki Chang Hong, Kwang Youn Whang, Young Sik Lee

Research output: Contribution to journal › Article › peer-review

33 Citations (Scopus)

Abstract

MicroRNAs (miRNAs) are an abundant class of small regulatory RNAs that regulate the stability and translation of cognate mRNAs. Although an increasing number of porcine miRNAs has recently been identified, the full repertoire of miRNAs in pig remains to be elucidated. To identify porcine miRNAs potentially involved in myogenesis and adipogenesis, we constructed small RNA cDNA libraries from skeletal muscle and adipose tissue and identified 89 distinct miRNAs that are conserved in pig, of which 15 were new. Expression analysis of all newly identified and selected known porcine miRNAs revealed that some miRNAs were enriched in a tissue-specific manner, whereas others were expressed ubiquitously in the porcine tissues examined. Our results expand the number of known porcine miRNAs and provide useful information for further investigating the biological functions of miRNAs associated with growth and development of skeletal muscle or adipose tissue in pig.

Original language	English
Pages (from-to)	3567-3574
Number of pages	8
Journal	Molecular biology reports
Volume	37
Issue number	7
DOIs	https://doi.org/10.1007/s11033-010-0005-6
Publication status	Published - 2010 Oct

Bibliographical note

Funding Information:
Acknowledgments We thank Jun Mo Kim and Jong-Gun Kim for assistance in collecting porcine tissue samples. This work was supported by a grant (20070301034036) from the BioGreen 21 Program, Rural Development Administration, Republic of Korea.

Keywords

Adipose tissue
MicroRNA
Pig
Skeletal muscle

ASJC Scopus subject areas

Molecular Biology
Genetics

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10.1007/s11033-010-0005-6

Cite this

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abstract = "MicroRNAs (miRNAs) are an abundant class of small regulatory RNAs that regulate the stability and translation of cognate mRNAs. Although an increasing number of porcine miRNAs has recently been identified, the full repertoire of miRNAs in pig remains to be elucidated. To identify porcine miRNAs potentially involved in myogenesis and adipogenesis, we constructed small RNA cDNA libraries from skeletal muscle and adipose tissue and identified 89 distinct miRNAs that are conserved in pig, of which 15 were new. Expression analysis of all newly identified and selected known porcine miRNAs revealed that some miRNAs were enriched in a tissue-specific manner, whereas others were expressed ubiquitously in the porcine tissues examined. Our results expand the number of known porcine miRNAs and provide useful information for further investigating the biological functions of miRNAs associated with growth and development of skeletal muscle or adipose tissue in pig.",

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author = "Cho, {Ik Sang} and Jung Kim and Seo, {Hye Youn} and Lim, {Do Hwan} and Hong, {Jae Sang} and Park, {Young Hee} and Park, {Dae Cheul} and Hong, {Ki Chang} and Whang, {Kwang Youn} and Lee, {Young Sik}",

note = "Funding Information: Acknowledgments We thank Jun Mo Kim and Jong-Gun Kim for assistance in collecting porcine tissue samples. This work was supported by a grant (20070301034036) from the BioGreen 21 Program, Rural Development Administration, Republic of Korea.",

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AU - Lim, Do Hwan

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AU - Park, Young Hee

AU - Park, Dae Cheul

AU - Hong, Ki Chang

AU - Whang, Kwang Youn

AU - Lee, Young Sik

N1 - Funding Information: Acknowledgments We thank Jun Mo Kim and Jong-Gun Kim for assistance in collecting porcine tissue samples. This work was supported by a grant (20070301034036) from the BioGreen 21 Program, Rural Development Administration, Republic of Korea.

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KW - Adipose tissue

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Cloning and characterization of microRNAs from porcine skeletal muscle and adipose tissue

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

Access to Document

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