Cloning and expression analysis of chicken phospholipid-hydroperoxide glutathione peroxidase

Byung Whi Kong; Hyunggee Kim; Douglas N. Foster

doi:10.1081/ABIO-120020183

Cloning and expression analysis of chicken phospholipid-hydroperoxide glutathione peroxidase

Byung Whi Kong, Hyunggee Kim, Douglas N. Foster

Research output: Contribution to journal › Article › peer-review

3 Citations (Scopus)

Abstract

Phospholipid-hydroperoxide glutathione peroxidase (GPX4 or PHGPX) is a unique selenium dependent glutathione peroxidase that reduces phospholipid, cholesterol, and cholesteryl ester hydroperoxides. Phospholipid-hydroperoxide glutathione peroxidase has been shown to exist as both a 197 amino acid mitochondrial targeting protein and as a 170 amino acid non-mitochondrial protein. The cDNA encoding the non-mitochondrial chicken GPX4 (cGPX4) has been isolated from an immortalized DF-1 chicken embryonic fibroblast (CEF) cell line cDNA library. The nucleotide sequence of cGPX4 was 802 bp in length with an open reading frame (ORF) that encoded 170 amino acids but lacked the N-terminal domain that encoded the mitochondrial leader sequence (MLS). Chicken non-mitochondrial GPX4 was highly expressed in brain and stromal tissues. Surprisingly, it was found that ovarian stromal tissue cGPX4 expression is regulated quite differently according to the reproductive status of the bird, suggesting that GPX4 may play an important role in reproduction in response to steroid hormones, in addition to its general antioxidant functions.

Original language	English
Pages (from-to)	19-29
Number of pages	11
Journal	Animal Biotechnology
Volume	14
Issue number	1
DOIs	https://doi.org/10.1081/ABIO-120020183
Publication status	Published - 2003 May
Externally published	Yes

Keywords

Antioxidant
Chicken
PHGPX
Reproductive cycle expression of PHGPX
Selenium dependent protein

ASJC Scopus subject areas

Biotechnology
Bioengineering
Animal Science and Zoology

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10.1081/ABIO-120020183

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title = "Cloning and expression analysis of chicken phospholipid-hydroperoxide glutathione peroxidase",

abstract = "Phospholipid-hydroperoxide glutathione peroxidase (GPX4 or PHGPX) is a unique selenium dependent glutathione peroxidase that reduces phospholipid, cholesterol, and cholesteryl ester hydroperoxides. Phospholipid-hydroperoxide glutathione peroxidase has been shown to exist as both a 197 amino acid mitochondrial targeting protein and as a 170 amino acid non-mitochondrial protein. The cDNA encoding the non-mitochondrial chicken GPX4 (cGPX4) has been isolated from an immortalized DF-1 chicken embryonic fibroblast (CEF) cell line cDNA library. The nucleotide sequence of cGPX4 was 802 bp in length with an open reading frame (ORF) that encoded 170 amino acids but lacked the N-terminal domain that encoded the mitochondrial leader sequence (MLS). Chicken non-mitochondrial GPX4 was highly expressed in brain and stromal tissues. Surprisingly, it was found that ovarian stromal tissue cGPX4 expression is regulated quite differently according to the reproductive status of the bird, suggesting that GPX4 may play an important role in reproduction in response to steroid hormones, in addition to its general antioxidant functions.",

keywords = "Antioxidant, Chicken, PHGPX, Reproductive cycle expression of PHGPX, Selenium dependent protein",

author = "Kong, {Byung Whi} and Hyunggee Kim and Foster, {Douglas N.}",

note = "Funding Information: We thank Jim Farris, Linda Foster, and Shelly Christman (University of Minnesota) for their helpful discussions. This work was supported, in part, by USDA=NRICGP grant no. 9603280 and a grant from Syntro=Schering Plough to D.N.F.",

year = "2003",

month = may,

doi = "10.1081/ABIO-120020183",

language = "English",

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pages = "19--29",

journal = "Animal Biotechnology",

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AU - Kong, Byung Whi

AU - Kim, Hyunggee

AU - Foster, Douglas N.

N1 - Funding Information: We thank Jim Farris, Linda Foster, and Shelly Christman (University of Minnesota) for their helpful discussions. This work was supported, in part, by USDA=NRICGP grant no. 9603280 and a grant from Syntro=Schering Plough to D.N.F.

PY - 2003/5

Y1 - 2003/5

N2 - Phospholipid-hydroperoxide glutathione peroxidase (GPX4 or PHGPX) is a unique selenium dependent glutathione peroxidase that reduces phospholipid, cholesterol, and cholesteryl ester hydroperoxides. Phospholipid-hydroperoxide glutathione peroxidase has been shown to exist as both a 197 amino acid mitochondrial targeting protein and as a 170 amino acid non-mitochondrial protein. The cDNA encoding the non-mitochondrial chicken GPX4 (cGPX4) has been isolated from an immortalized DF-1 chicken embryonic fibroblast (CEF) cell line cDNA library. The nucleotide sequence of cGPX4 was 802 bp in length with an open reading frame (ORF) that encoded 170 amino acids but lacked the N-terminal domain that encoded the mitochondrial leader sequence (MLS). Chicken non-mitochondrial GPX4 was highly expressed in brain and stromal tissues. Surprisingly, it was found that ovarian stromal tissue cGPX4 expression is regulated quite differently according to the reproductive status of the bird, suggesting that GPX4 may play an important role in reproduction in response to steroid hormones, in addition to its general antioxidant functions.

AB - Phospholipid-hydroperoxide glutathione peroxidase (GPX4 or PHGPX) is a unique selenium dependent glutathione peroxidase that reduces phospholipid, cholesterol, and cholesteryl ester hydroperoxides. Phospholipid-hydroperoxide glutathione peroxidase has been shown to exist as both a 197 amino acid mitochondrial targeting protein and as a 170 amino acid non-mitochondrial protein. The cDNA encoding the non-mitochondrial chicken GPX4 (cGPX4) has been isolated from an immortalized DF-1 chicken embryonic fibroblast (CEF) cell line cDNA library. The nucleotide sequence of cGPX4 was 802 bp in length with an open reading frame (ORF) that encoded 170 amino acids but lacked the N-terminal domain that encoded the mitochondrial leader sequence (MLS). Chicken non-mitochondrial GPX4 was highly expressed in brain and stromal tissues. Surprisingly, it was found that ovarian stromal tissue cGPX4 expression is regulated quite differently according to the reproductive status of the bird, suggesting that GPX4 may play an important role in reproduction in response to steroid hormones, in addition to its general antioxidant functions.

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KW - Reproductive cycle expression of PHGPX

KW - Selenium dependent protein

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Cloning and expression analysis of chicken phospholipid-hydroperoxide glutathione peroxidase

Abstract

Keywords

ASJC Scopus subject areas

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