Cloning and expression of male-specific protein (MSP) from the hemolymph of greater wax moth, Galleria mellonella L.

Jikhyon Han, Chang Seok Lee, Chi Young Yun, Bong Hee Lee, Young Gyu Ko, Chang Soo Kang, Sang Dae Lee, Jae Sam Hwang, Seok Woo Kang, Hak R. Kim

    Research output: Contribution to journalArticlepeer-review

    5 Citations (Scopus)

    Abstract

    Male-specific protein (MSP) is a soluble protein that accumulates in high amounts in the hemolymph and other organs of adult male wax moth. The MSP was purified from adult male wax moth by gel filtration and reversed phase column chromatography, and its amino acid sequence was determined. Because of blocked N-terminus, several internal amino acid sequences of MSP were obtained by the in-gel digestion method using trypsin. RT-PCR was conducted using degenerate primers designed from the internal amino add sequences. 5′-RACE PCR was used to obtain the complete coding region and 5′-UTR sequence. The full length MSP cDNA sequence encodes a 239 amino acid polypeptide with an 18 amino acid signal peptide. The putative mature MSP has a molecular mass of 24,317 Da and an isoelectric point (pl) of 6.00, but shows a molecular mass of 27 kDa on SDS-PAGE. Sequence alignment showed a significant similarity between MSP and juvenile hormone binding proteins (JHBPs) of several lepidopteran species, including G. mellonella.

    Original languageEnglish
    Pages (from-to)110-120
    Number of pages11
    JournalArchives of Insect Biochemistry and Physiology
    Volume54
    Issue number3
    DOIs
    Publication statusPublished - 2003 Nov

    Keywords

    • Galleria mellonella
    • Juvenile hormone binding protein
    • MSP
    • Mole-specific protein
    • cDNA

    ASJC Scopus subject areas

    • Insect Science
    • Physiology
    • Biochemistry

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