Cloning and heterologous expression of a novel insecticidal gene (tccC1) from Xenorhabdus nematophilus strain

Pom Joo Lee; Ji Young Ahn; Yang Hoon Kim; Seung Wook Kim; Ji Yeon Kim; Jae Sung Park; Jeewon Lee

doi:10.1016/j.bbrc.2004.04.203

Cloning and heterologous expression of a novel insecticidal gene (tccC1) from Xenorhabdus nematophilus strain

Pom Joo Lee, Ji Young Ahn, Yang Hoon Kim, Seung Wook Kim, Ji Yeon Kim, Jae Sung Park, Jeewon Lee

Research output: Contribution to journal › Article › peer-review

24 Citations (Scopus)

Abstract

We have identified and cloned a novel toxin gene (tccC1/xptB1) from Xenorhabdus nematophilus strain isolated from Korea-specific entomophagous nematode Steinernema glaseri MK. The DNA sequence of cloned toxin gene (3048bp) has an open reading frame encoding 1016 amino acids with a predicted molecular mass of 111058Da. The toxin sequence shares 50-96% identical amino acid residues with the previously reported tccC1 cloned from X. nematophilus (AJ308438), Photorhabdus luminescens W14 (AF346499) P. luminescens TTO1 (BX571873), and Yersinia pestis CO92 (NC_003143). The toxin gene was successfully expressed in Escherichia coli, and the recombinant toxin protein caused a rapid cessation in mortality of Galleria mellonella larvae (80% death of larvae within 2 days). Conclusively, the heterologous expression of the novel gene tccC1 cloned into E. coli plasmid vector produced recombinant toxin with high insecticidal activity.

Original language	English
Pages (from-to)	1110-1116
Number of pages	7
Journal	Biochemical and biophysical research communications
Volume	319
Issue number	4
DOIs	https://doi.org/10.1016/j.bbrc.2004.04.203
Publication status	Published - 2004 Jul 9

Bibliographical note

Funding Information:
This work was supported by Ministry of Commerce, Industry, and Energy in South Korea.

Keywords

Heterologous expression
High insecticidal activity
Toxin gene (tccC1/XptB1)
Xenorhabdus nematophilus

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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10.1016/j.bbrc.2004.04.203

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title = "Cloning and heterologous expression of a novel insecticidal gene (tccC1) from Xenorhabdus nematophilus strain",

abstract = "We have identified and cloned a novel toxin gene (tccC1/xptB1) from Xenorhabdus nematophilus strain isolated from Korea-specific entomophagous nematode Steinernema glaseri MK. The DNA sequence of cloned toxin gene (3048bp) has an open reading frame encoding 1016 amino acids with a predicted molecular mass of 111058Da. The toxin sequence shares 50-96% identical amino acid residues with the previously reported tccC1 cloned from X. nematophilus (AJ308438), Photorhabdus luminescens W14 (AF346499) P. luminescens TTO1 (BX571873), and Yersinia pestis CO92 (NC_003143). The toxin gene was successfully expressed in Escherichia coli, and the recombinant toxin protein caused a rapid cessation in mortality of Galleria mellonella larvae (80% death of larvae within 2 days). Conclusively, the heterologous expression of the novel gene tccC1 cloned into E. coli plasmid vector produced recombinant toxin with high insecticidal activity.",

keywords = "Heterologous expression, High insecticidal activity, Toxin gene (tccC1/XptB1), Xenorhabdus nematophilus",

author = "Lee, {Pom Joo} and Ahn, {Ji Young} and Kim, {Yang Hoon} and Kim, {Seung Wook} and Kim, {Ji Yeon} and Park, {Jae Sung} and Jeewon Lee",

note = "Funding Information: This work was supported by Ministry of Commerce, Industry, and Energy in South Korea.",

year = "2004",

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doi = "10.1016/j.bbrc.2004.04.203",

language = "English",

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AU - Lee, Pom Joo

AU - Ahn, Ji Young

AU - Kim, Yang Hoon

AU - Kim, Seung Wook

AU - Kim, Ji Yeon

AU - Park, Jae Sung

AU - Lee, Jeewon

N1 - Funding Information: This work was supported by Ministry of Commerce, Industry, and Energy in South Korea.

PY - 2004/7/9

Y1 - 2004/7/9

N2 - We have identified and cloned a novel toxin gene (tccC1/xptB1) from Xenorhabdus nematophilus strain isolated from Korea-specific entomophagous nematode Steinernema glaseri MK. The DNA sequence of cloned toxin gene (3048bp) has an open reading frame encoding 1016 amino acids with a predicted molecular mass of 111058Da. The toxin sequence shares 50-96% identical amino acid residues with the previously reported tccC1 cloned from X. nematophilus (AJ308438), Photorhabdus luminescens W14 (AF346499) P. luminescens TTO1 (BX571873), and Yersinia pestis CO92 (NC_003143). The toxin gene was successfully expressed in Escherichia coli, and the recombinant toxin protein caused a rapid cessation in mortality of Galleria mellonella larvae (80% death of larvae within 2 days). Conclusively, the heterologous expression of the novel gene tccC1 cloned into E. coli plasmid vector produced recombinant toxin with high insecticidal activity.

AB - We have identified and cloned a novel toxin gene (tccC1/xptB1) from Xenorhabdus nematophilus strain isolated from Korea-specific entomophagous nematode Steinernema glaseri MK. The DNA sequence of cloned toxin gene (3048bp) has an open reading frame encoding 1016 amino acids with a predicted molecular mass of 111058Da. The toxin sequence shares 50-96% identical amino acid residues with the previously reported tccC1 cloned from X. nematophilus (AJ308438), Photorhabdus luminescens W14 (AF346499) P. luminescens TTO1 (BX571873), and Yersinia pestis CO92 (NC_003143). The toxin gene was successfully expressed in Escherichia coli, and the recombinant toxin protein caused a rapid cessation in mortality of Galleria mellonella larvae (80% death of larvae within 2 days). Conclusively, the heterologous expression of the novel gene tccC1 cloned into E. coli plasmid vector produced recombinant toxin with high insecticidal activity.

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Cloning and heterologous expression of a novel insecticidal gene (tccC1) from Xenorhabdus nematophilus strain

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

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