TY - JOUR
T1 - Cloning and localization of rgpr85 encoding rat G-protein-coupled receptor
AU - Jeon, Jongrye
AU - Kim, Changmee
AU - Sun, Woong
AU - Chung, Hyesun
AU - Park, Sun Hwa
AU - Kim, Hyun
N1 - Funding Information:
We thank J. Chin and M. Park for animal preparation and in situ hybridization histochemistry. We also thank Dr. S.D. Kim for the review of manuscript. This work was supported by Grant M1-0108-00-0071 from Korean Ministry of Science and Technology.
PY - 2002
Y1 - 2002
N2 - In an attempt to isolate genes involved in the brain development using ordered differential display PCR, we cloned rgpr85 which encodes rat G-protein-coupled receptor with high degree of identity to the amine-like neurotransmitter receptors. This gene was found to be localized at rat chromosome 4q21. In situ hybridization demonstrated that rgpr85 was predominantly expressed in the developing brain and spinal cord. Hybridization signal was especially abundant within the embryonic cortical plates where post-mitotic cortical neurons are localized. In the cerebral cortex, the expression of rgpr85 was gradually decreased postnatally and became undetectable by P18. However, weak but significant expression of rgpr85 was maintained in the adult hippocampal formation, olfactory bulb, and cerebellum. Interestingly, rgpr85 expression was transiently induced in the adult hippocampal formation, piriform cortex, and amygdaloid complex by kainic acid (KA) treatment. Thus, dynamic regulation of rgpr85 expression suggests an importance of rgpr85-mediated signaling in the development of cerebral cortex and in the KA-induced responses in the adult brain.
AB - In an attempt to isolate genes involved in the brain development using ordered differential display PCR, we cloned rgpr85 which encodes rat G-protein-coupled receptor with high degree of identity to the amine-like neurotransmitter receptors. This gene was found to be localized at rat chromosome 4q21. In situ hybridization demonstrated that rgpr85 was predominantly expressed in the developing brain and spinal cord. Hybridization signal was especially abundant within the embryonic cortical plates where post-mitotic cortical neurons are localized. In the cerebral cortex, the expression of rgpr85 was gradually decreased postnatally and became undetectable by P18. However, weak but significant expression of rgpr85 was maintained in the adult hippocampal formation, olfactory bulb, and cerebellum. Interestingly, rgpr85 expression was transiently induced in the adult hippocampal formation, piriform cortex, and amygdaloid complex by kainic acid (KA) treatment. Thus, dynamic regulation of rgpr85 expression suggests an importance of rgpr85-mediated signaling in the development of cerebral cortex and in the KA-induced responses in the adult brain.
KW - Development of cerebral cortex
KW - In situ hybridization
KW - Kainic acid
KW - ODD-PCR
KW - Rat chromosome 4q21
KW - Rgpr85
UR - http://www.scopus.com/inward/record.url?scp=0036429071&partnerID=8YFLogxK
U2 - 10.1016/S0006-291X(02)02515-9
DO - 10.1016/S0006-291X(02)02515-9
M3 - Article
C2 - 12408996
AN - SCOPUS:0036429071
SN - 0006-291X
VL - 298
SP - 613
EP - 618
JO - Biochemical and biophysical research communications
JF - Biochemical and biophysical research communications
IS - 4
ER -