CM1, a possible novel activation molecule on human lymphocytes

Dae Y. Hur; Seonghan Kim; Young In Kim; Hye Y. Min; Dae J. Kim; Dong Sup Lee; Daeho Cho; Young Il Hwang; Douk H. Hwang; Seong H. Park; Hee K. Ahn; Ka Y. Chang; Yoon B. Kim; Wang J. Lee

doi:10.1016/S0165-2478(00)00201-7

CM1, a possible novel activation molecule on human lymphocytes

Dae Y. Hur, Seonghan Kim, Young In Kim, Hye Y. Min, Dae J. Kim, Dong Sup Lee, Daeho Cho, Young Il Hwang, Douk H. Hwang, Seong H. Park, Hee K. Ahn, Ka Y. Chang, Yoon B. Kim, Wang J. Lee

Research output: Contribution to journal › Article › peer-review

2 Citations (Scopus)

Abstract

CM1 (centrocyte/-blast marker 1) defined by a mAb developed against concanavalin-A activated PBMC, is expressed specifically on some tonsillar germinal center (GC) B cells. In single flow cytometric analysis, the bone marrow did not express these molecules nor did the PBMC or the thymocytes. The peripheral B lymphocytes showed more than 90% positive, while the peripheral T lymphocytes showed approximately 60% positive at 48 h after activation by PMA/ionomycin, respectively. A western blot analysis and an immunoprecipitation for CM1 showed a band at 70 kDa. Cross-linking of CM1 with anti-CM1 mAb induced apoptosis of the GC B cells (CD38⁺IgD^-). Immunohistochemical staining revealed that the CM1 molecule is distributed over the entire area except the proximal dark zone of the tonsillar germinal centers. These results suggest that the CM1 molecule might be involved in differentiation of the germinal center B cells as one of the novel centrocyte markers. (C) 2000 Elsevier Science B.V.

Original language	English
Pages (from-to)	95-102
Number of pages	8
Journal	Immunology Letters
Volume	74
Issue number	2
DOIs	https://doi.org/10.1016/S0165-2478(00)00201-7
Publication status	Published - 2000 Oct 3
Externally published	Yes

Keywords

Activation molecule
Apoptosis
CM1
Human lymphocytes

ASJC Scopus subject areas

Immunology and Allergy
Immunology

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10.1016/S0165-2478(00)00201-7

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@article{117834a17558403b9ec67410dc4ec110,

title = "CM1, a possible novel activation molecule on human lymphocytes",

abstract = "CM1 (centrocyte/-blast marker 1) defined by a mAb developed against concanavalin-A activated PBMC, is expressed specifically on some tonsillar germinal center (GC) B cells. In single flow cytometric analysis, the bone marrow did not express these molecules nor did the PBMC or the thymocytes. The peripheral B lymphocytes showed more than 90% positive, while the peripheral T lymphocytes showed approximately 60% positive at 48 h after activation by PMA/ionomycin, respectively. A western blot analysis and an immunoprecipitation for CM1 showed a band at 70 kDa. Cross-linking of CM1 with anti-CM1 mAb induced apoptosis of the GC B cells (CD38+IgD-). Immunohistochemical staining revealed that the CM1 molecule is distributed over the entire area except the proximal dark zone of the tonsillar germinal centers. These results suggest that the CM1 molecule might be involved in differentiation of the germinal center B cells as one of the novel centrocyte markers. (C) 2000 Elsevier Science B.V.",

keywords = "Activation molecule, Apoptosis, CM1, Human lymphocytes",

author = "Hur, {Dae Y.} and Seonghan Kim and Kim, {Young In} and Min, {Hye Y.} and Kim, {Dae J.} and Lee, {Dong Sup} and Daeho Cho and Hwang, {Young Il} and Hwang, {Douk H.} and Park, {Seong H.} and Ahn, {Hee K.} and Chang, {Ka Y.} and Kim, {Yoon B.} and Lee, {Wang J.}",

note = "Funding Information: We gratefully acknowledge the advice and gift (HJ2 mAb) of Dr M. Nahm. We also thank Dr Chang J. Kang for critically reviewing the manuscript. D.Y. Hur and S. Kim contributed equally to this paper. This study was supported by G7 Biomedical Engineering Grant (HMP-98-G-2-047) of Ministry of Health and Welfare of Korea.",

year = "2000",

month = oct,

day = "3",

doi = "10.1016/S0165-2478(00)00201-7",

language = "English",

volume = "74",

pages = "95--102",

journal = "Immunology Letters",

issn = "0165-2478",

publisher = "Elsevier",

number = "2",

}

TY - JOUR

T1 - CM1, a possible novel activation molecule on human lymphocytes

AU - Hur, Dae Y.

AU - Kim, Seonghan

AU - Kim, Young In

AU - Min, Hye Y.

AU - Kim, Dae J.

AU - Lee, Dong Sup

AU - Cho, Daeho

AU - Hwang, Young Il

AU - Hwang, Douk H.

AU - Park, Seong H.

AU - Ahn, Hee K.

AU - Chang, Ka Y.

AU - Kim, Yoon B.

AU - Lee, Wang J.

N1 - Funding Information: We gratefully acknowledge the advice and gift (HJ2 mAb) of Dr M. Nahm. We also thank Dr Chang J. Kang for critically reviewing the manuscript. D.Y. Hur and S. Kim contributed equally to this paper. This study was supported by G7 Biomedical Engineering Grant (HMP-98-G-2-047) of Ministry of Health and Welfare of Korea.

PY - 2000/10/3

Y1 - 2000/10/3

N2 - CM1 (centrocyte/-blast marker 1) defined by a mAb developed against concanavalin-A activated PBMC, is expressed specifically on some tonsillar germinal center (GC) B cells. In single flow cytometric analysis, the bone marrow did not express these molecules nor did the PBMC or the thymocytes. The peripheral B lymphocytes showed more than 90% positive, while the peripheral T lymphocytes showed approximately 60% positive at 48 h after activation by PMA/ionomycin, respectively. A western blot analysis and an immunoprecipitation for CM1 showed a band at 70 kDa. Cross-linking of CM1 with anti-CM1 mAb induced apoptosis of the GC B cells (CD38+IgD-). Immunohistochemical staining revealed that the CM1 molecule is distributed over the entire area except the proximal dark zone of the tonsillar germinal centers. These results suggest that the CM1 molecule might be involved in differentiation of the germinal center B cells as one of the novel centrocyte markers. (C) 2000 Elsevier Science B.V.

AB - CM1 (centrocyte/-blast marker 1) defined by a mAb developed against concanavalin-A activated PBMC, is expressed specifically on some tonsillar germinal center (GC) B cells. In single flow cytometric analysis, the bone marrow did not express these molecules nor did the PBMC or the thymocytes. The peripheral B lymphocytes showed more than 90% positive, while the peripheral T lymphocytes showed approximately 60% positive at 48 h after activation by PMA/ionomycin, respectively. A western blot analysis and an immunoprecipitation for CM1 showed a band at 70 kDa. Cross-linking of CM1 with anti-CM1 mAb induced apoptosis of the GC B cells (CD38+IgD-). Immunohistochemical staining revealed that the CM1 molecule is distributed over the entire area except the proximal dark zone of the tonsillar germinal centers. These results suggest that the CM1 molecule might be involved in differentiation of the germinal center B cells as one of the novel centrocyte markers. (C) 2000 Elsevier Science B.V.

KW - Activation molecule

KW - Apoptosis

KW - CM1

KW - Human lymphocytes

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U2 - 10.1016/S0165-2478(00)00201-7

DO - 10.1016/S0165-2478(00)00201-7

M3 - Article

C2 - 10996383

AN - SCOPUS:0034601875

SN - 0165-2478

VL - 74

SP - 95

EP - 102

JO - Immunology Letters

JF - Immunology Letters

IS - 2

ER -

CM1, a possible novel activation molecule on human lymphocytes

Abstract

Keywords

ASJC Scopus subject areas

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