Co-culture of human bone marrow mesenchymal stem cells and macrophages attenuates lipopolysaccharide-induced inflammation in human corneal epithelial cells

Won Yong Jeong; Ji Hye Kim; Chan Wha Kim

doi:10.1080/09168451.2018.1438167

Co-culture of human bone marrow mesenchymal stem cells and macrophages attenuates lipopolysaccharide-induced inflammation in human corneal epithelial cells

Won Yong Jeong
, Ji Hye Kim
, Chan Wha Kim^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

9 Citations (Scopus)

Abstract

Dry eye syndrome (DES) is considered as an ocular surface inflammatory disease. Previous studies have shown inflammation plays an important role in the progression and onset of DES. Co-culture of human bone marrow mesenchymal stem cells (HBMSCs) and macrophages showed immunomodulatory effects via regulation of cytokine regulation. Thus, the aim of this study was to investigate the effect of the interaction of these cells on in vitro DES model. The conditioned media (CM) from macrophages, HBMSCs, and HBMSCs + macrophages were treated to human corneal epithelial cells, which showed significant reduction in IL-1α and IL-1β expression levels in HBMSCs + macrophages group. Moreover, the IL-1 Receptor Antagonist (IL-1RA) was highly expressed in the CM from the HBMSCs + macrophages group. Wounded eyes of mice were treated with IL-1RA at 0–100 ng/mL for 16 h, the wound size was reduced. The results of this study might lead to the identification of new therapeutic targets for DES.

Original language	English
Pages (from-to)	800-809
Number of pages	10
Journal	Bioscience, Biotechnology and Biochemistry
Volume	82
Issue number	5
DOIs	https://doi.org/10.1080/09168451.2018.1438167
Publication status	Published - 2018

Bibliographical note

Funding Information:
This work was supported by Korea University, with additional support from Korea University BK21 Plus Program (School of Life Sciences and Biotechnology, Korea University).

Publisher Copyright:
© 2018 Japan society for Bioscience, Biotechnology, and agrochemistry.

Keywords

Dry eye syndrome
Human corneal epithelial cells
Il-1 receptor antagonist
Mesenchymal stem cells

ASJC Scopus subject areas

Biotechnology
Analytical Chemistry
Biochemistry
Applied Microbiology and Biotechnology
Molecular Biology
Organic Chemistry

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1080/09168451.2018.1438167

Cite this

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title = "Co-culture of human bone marrow mesenchymal stem cells and macrophages attenuates lipopolysaccharide-induced inflammation in human corneal epithelial cells",

abstract = "Dry eye syndrome (DES) is considered as an ocular surface inflammatory disease. Previous studies have shown inflammation plays an important role in the progression and onset of DES. Co-culture of human bone marrow mesenchymal stem cells (HBMSCs) and macrophages showed immunomodulatory effects via regulation of cytokine regulation. Thus, the aim of this study was to investigate the effect of the interaction of these cells on in vitro DES model. The conditioned media (CM) from macrophages, HBMSCs, and HBMSCs + macrophages were treated to human corneal epithelial cells, which showed significant reduction in IL-1α and IL-1β expression levels in HBMSCs + macrophages group. Moreover, the IL-1 Receptor Antagonist (IL-1RA) was highly expressed in the CM from the HBMSCs + macrophages group. Wounded eyes of mice were treated with IL-1RA at 0–100 ng/mL for 16 h, the wound size was reduced. The results of this study might lead to the identification of new therapeutic targets for DES.",

keywords = "Dry eye syndrome, Human corneal epithelial cells, Il-1 receptor antagonist, Mesenchymal stem cells",

author = "Jeong, \{Won Yong\} and Kim, \{Ji Hye\} and Kim, \{Chan Wha\}",

note = "Funding Information: This work was supported by Korea University, with additional support from Korea University BK21 Plus Program (School of Life Sciences and Biotechnology, Korea University). Publisher Copyright: {\textcopyright} 2018 Japan society for Bioscience, Biotechnology, and agrochemistry.",

year = "2018",

doi = "10.1080/09168451.2018.1438167",

language = "English",

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T1 - Co-culture of human bone marrow mesenchymal stem cells and macrophages attenuates lipopolysaccharide-induced inflammation in human corneal epithelial cells

AU - Jeong, Won Yong

AU - Kim, Ji Hye

AU - Kim, Chan Wha

N1 - Funding Information: This work was supported by Korea University, with additional support from Korea University BK21 Plus Program (School of Life Sciences and Biotechnology, Korea University). Publisher Copyright: © 2018 Japan society for Bioscience, Biotechnology, and agrochemistry.

PY - 2018

Y1 - 2018

N2 - Dry eye syndrome (DES) is considered as an ocular surface inflammatory disease. Previous studies have shown inflammation plays an important role in the progression and onset of DES. Co-culture of human bone marrow mesenchymal stem cells (HBMSCs) and macrophages showed immunomodulatory effects via regulation of cytokine regulation. Thus, the aim of this study was to investigate the effect of the interaction of these cells on in vitro DES model. The conditioned media (CM) from macrophages, HBMSCs, and HBMSCs + macrophages were treated to human corneal epithelial cells, which showed significant reduction in IL-1α and IL-1β expression levels in HBMSCs + macrophages group. Moreover, the IL-1 Receptor Antagonist (IL-1RA) was highly expressed in the CM from the HBMSCs + macrophages group. Wounded eyes of mice were treated with IL-1RA at 0–100 ng/mL for 16 h, the wound size was reduced. The results of this study might lead to the identification of new therapeutic targets for DES.

AB - Dry eye syndrome (DES) is considered as an ocular surface inflammatory disease. Previous studies have shown inflammation plays an important role in the progression and onset of DES. Co-culture of human bone marrow mesenchymal stem cells (HBMSCs) and macrophages showed immunomodulatory effects via regulation of cytokine regulation. Thus, the aim of this study was to investigate the effect of the interaction of these cells on in vitro DES model. The conditioned media (CM) from macrophages, HBMSCs, and HBMSCs + macrophages were treated to human corneal epithelial cells, which showed significant reduction in IL-1α and IL-1β expression levels in HBMSCs + macrophages group. Moreover, the IL-1 Receptor Antagonist (IL-1RA) was highly expressed in the CM from the HBMSCs + macrophages group. Wounded eyes of mice were treated with IL-1RA at 0–100 ng/mL for 16 h, the wound size was reduced. The results of this study might lead to the identification of new therapeutic targets for DES.

KW - Dry eye syndrome

KW - Human corneal epithelial cells

KW - Il-1 receptor antagonist

KW - Mesenchymal stem cells

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DO - 10.1080/09168451.2018.1438167

M3 - Article

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SN - 0916-8451

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EP - 809

JO - Bioscience, Biotechnology and Biochemistry

JF - Bioscience, Biotechnology and Biochemistry

IS - 5

ER -

Co-culture of human bone marrow mesenchymal stem cells and macrophages attenuates lipopolysaccharide-induced inflammation in human corneal epithelial cells

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

UN SDGs

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