Co-immobilization of Candida rugosa and Rhyzopus oryzae lipases and biodiesel production

Jong Ho Lee, Sung Bong Kim, Hah Young Yoo, Ja Hyun Lee, Sung Ok Han, Chulhwan Park, Seung Wook Kim

Research output: Contribution to journalArticlepeer-review

43 Citations (Scopus)


Candida rugosa lipase and Ryzopus oryzae lipase were simultaneously immobilized on silica gel following enzyme pretreatment. The factors affecting the co-immobilization process, such as reaction time and enzyme ratio, were investigated. Biodiesel was then produced by using the co-immobilized enzyme matrix. A batch system was employed with stepwise methanol feeding, and the continuous process involved a packed-bed reactor. Under optimal immobilization conditions, the activity was approximately 16,000 U/g·matrix. When co-immobilized enzyme was used with optimized stepwise methanol feeding, conversion of biodiesel reached about 99% at 3 h and was maintained at a level of over 90% for about 30 reuses.

Original languageEnglish
Pages (from-to)1335-1338
Number of pages4
JournalKorean Journal of Chemical Engineering
Issue number6
Publication statusPublished - 2013 Jun

Bibliographical note

Funding Information:
This work was supported by the Advanced Biomass R&D Center (ABC) of Korea Grant funded the Ministry of Education, Science, and Technology (2010-0029799).


  • Biodiesel
  • Co-immobilization
  • Enzymatic Process
  • Lipase

ASJC Scopus subject areas

  • General Chemistry
  • General Chemical Engineering


Dive into the research topics of 'Co-immobilization of Candida rugosa and Rhyzopus oryzae lipases and biodiesel production'. Together they form a unique fingerprint.

Cite this