Coagulation-Inspired Direct Fibrinogen Assay Using Plasmonic Nanoparticles Functionalized with Red Blood Cell Membranes

Insu Kim; Dongtak Lee; Sang Won Lee; Jeong Hoon Lee; Gyudo Lee; Dae Sung Yoon

doi:10.1021/acsnano.0c08136

Coagulation-Inspired Direct Fibrinogen Assay Using Plasmonic Nanoparticles Functionalized with Red Blood Cell Membranes

Insu Kim, Dongtak Lee, Sang Won Lee, Jeong Hoon Lee, Gyudo Lee, Dae Sung Yoon

Research output: Contribution to journal › Article › peer-review

34 Citations (Scopus)

Abstract

The fast measurement of fibrinogen is essential in evaluating life-threatening sepsis and cardiovascular diseases. Here, we aim to utilize biomimetic plasmonic Au nanoparticles using red blood cell membranes (RBCM-AuNPs) and demonstrate nanoscale coagulation-inspired fibrinogen detection via cross-linking between RBCM-AuNPs. The proposed biomimetic RBCM-AuNPs are highly suitable for fibrinogen detection because hemagglutination, occurring in the presence of fibrinogen, induces a shift in the localized surface plasmon resonance of the NPs. Specifically, when the two ends of the fibrinogen protein are bound to receptors on separate RBCM-AuNPs, cross-linking of the RBCM-AuNPs occurs, yielding a corresponding plasmon shift within 10 min. This coagulation-inspired fibrinogen detection method, with a low sample volume, high selectivity, and high speed, could facilitate the diagnosis of sepsis and cardiovascular diseases.

Original language	English
Pages (from-to)	6386-6394
Number of pages	9
Journal	ACS nano
Volume	15
Issue number	4
DOIs	https://doi.org/10.1021/acsnano.0c08136
Publication status	Published - 2021 Apr 27

Bibliographical note

Funding Information:
This work was supported by the National Research Foundation of Korea (NRF) grant funded by the Korean Government (MSIP) (Grant No. NRF-2018M3C1B7020722, NRF-2019R1A2B5B01070617, NRF-2020R1A2C2102262, and NRF-2020R1A6A3A01096477). This study was also supported by the BK21 FOUR (Fostering Outstanding Universities for Research). J. H. Lee was supported by a research grant from Kwangwoon University in 2021.

Publisher Copyright:
© 2021 American Chemical Society.

Keywords

cardiovascular disease
coagulation-inspired
integrin αβ
localized surface plasmon resonance
red blood cell membrane

ASJC Scopus subject areas

General Materials Science
General Engineering
General Physics and Astronomy

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1021/acsnano.0c08136

Cite this

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title = "Coagulation-Inspired Direct Fibrinogen Assay Using Plasmonic Nanoparticles Functionalized with Red Blood Cell Membranes",

abstract = "The fast measurement of fibrinogen is essential in evaluating life-threatening sepsis and cardiovascular diseases. Here, we aim to utilize biomimetic plasmonic Au nanoparticles using red blood cell membranes (RBCM-AuNPs) and demonstrate nanoscale coagulation-inspired fibrinogen detection via cross-linking between RBCM-AuNPs. The proposed biomimetic RBCM-AuNPs are highly suitable for fibrinogen detection because hemagglutination, occurring in the presence of fibrinogen, induces a shift in the localized surface plasmon resonance of the NPs. Specifically, when the two ends of the fibrinogen protein are bound to receptors on separate RBCM-AuNPs, cross-linking of the RBCM-AuNPs occurs, yielding a corresponding plasmon shift within 10 min. This coagulation-inspired fibrinogen detection method, with a low sample volume, high selectivity, and high speed, could facilitate the diagnosis of sepsis and cardiovascular diseases.",

keywords = "cardiovascular disease, coagulation-inspired, integrin αβ, localized surface plasmon resonance, red blood cell membrane",

author = "Insu Kim and Dongtak Lee and Lee, {Sang Won} and Lee, {Jeong Hoon} and Gyudo Lee and Yoon, {Dae Sung}",

note = "Funding Information: This work was supported by the National Research Foundation of Korea (NRF) grant funded by the Korean Government (MSIP) (Grant No. NRF-2018M3C1B7020722, NRF-2019R1A2B5B01070617, NRF-2020R1A2C2102262, and NRF-2020R1A6A3A01096477). This study was also supported by the BK21 FOUR (Fostering Outstanding Universities for Research). J. H. Lee was supported by a research grant from Kwangwoon University in 2021. Publisher Copyright: {\textcopyright} 2021 American Chemical Society.",

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N2 - The fast measurement of fibrinogen is essential in evaluating life-threatening sepsis and cardiovascular diseases. Here, we aim to utilize biomimetic plasmonic Au nanoparticles using red blood cell membranes (RBCM-AuNPs) and demonstrate nanoscale coagulation-inspired fibrinogen detection via cross-linking between RBCM-AuNPs. The proposed biomimetic RBCM-AuNPs are highly suitable for fibrinogen detection because hemagglutination, occurring in the presence of fibrinogen, induces a shift in the localized surface plasmon resonance of the NPs. Specifically, when the two ends of the fibrinogen protein are bound to receptors on separate RBCM-AuNPs, cross-linking of the RBCM-AuNPs occurs, yielding a corresponding plasmon shift within 10 min. This coagulation-inspired fibrinogen detection method, with a low sample volume, high selectivity, and high speed, could facilitate the diagnosis of sepsis and cardiovascular diseases.

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Coagulation-Inspired Direct Fibrinogen Assay Using Plasmonic Nanoparticles Functionalized with Red Blood Cell Membranes

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

UN SDGs

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