TY - JOUR
T1 - Coagulation-Inspired Direct Fibrinogen Assay Using Plasmonic Nanoparticles Functionalized with Red Blood Cell Membranes
AU - Kim, Insu
AU - Lee, Dongtak
AU - Lee, Sang Won
AU - Lee, Jeong Hoon
AU - Lee, Gyudo
AU - Yoon, Dae Sung
N1 - Funding Information:
This work was supported by the National Research Foundation of Korea (NRF) grant funded by the Korean Government (MSIP) (Grant No. NRF-2018M3C1B7020722, NRF-2019R1A2B5B01070617, NRF-2020R1A2C2102262, and NRF-2020R1A6A3A01096477). This study was also supported by the BK21 FOUR (Fostering Outstanding Universities for Research). J. H. Lee was supported by a research grant from Kwangwoon University in 2021.
Publisher Copyright:
© 2021 American Chemical Society.
PY - 2021/4/27
Y1 - 2021/4/27
N2 - The fast measurement of fibrinogen is essential in evaluating life-threatening sepsis and cardiovascular diseases. Here, we aim to utilize biomimetic plasmonic Au nanoparticles using red blood cell membranes (RBCM-AuNPs) and demonstrate nanoscale coagulation-inspired fibrinogen detection via cross-linking between RBCM-AuNPs. The proposed biomimetic RBCM-AuNPs are highly suitable for fibrinogen detection because hemagglutination, occurring in the presence of fibrinogen, induces a shift in the localized surface plasmon resonance of the NPs. Specifically, when the two ends of the fibrinogen protein are bound to receptors on separate RBCM-AuNPs, cross-linking of the RBCM-AuNPs occurs, yielding a corresponding plasmon shift within 10 min. This coagulation-inspired fibrinogen detection method, with a low sample volume, high selectivity, and high speed, could facilitate the diagnosis of sepsis and cardiovascular diseases.
AB - The fast measurement of fibrinogen is essential in evaluating life-threatening sepsis and cardiovascular diseases. Here, we aim to utilize biomimetic plasmonic Au nanoparticles using red blood cell membranes (RBCM-AuNPs) and demonstrate nanoscale coagulation-inspired fibrinogen detection via cross-linking between RBCM-AuNPs. The proposed biomimetic RBCM-AuNPs are highly suitable for fibrinogen detection because hemagglutination, occurring in the presence of fibrinogen, induces a shift in the localized surface plasmon resonance of the NPs. Specifically, when the two ends of the fibrinogen protein are bound to receptors on separate RBCM-AuNPs, cross-linking of the RBCM-AuNPs occurs, yielding a corresponding plasmon shift within 10 min. This coagulation-inspired fibrinogen detection method, with a low sample volume, high selectivity, and high speed, could facilitate the diagnosis of sepsis and cardiovascular diseases.
KW - cardiovascular disease
KW - coagulation-inspired
KW - integrin αβ
KW - localized surface plasmon resonance
KW - red blood cell membrane
UR - http://www.scopus.com/inward/record.url?scp=85100660544&partnerID=8YFLogxK
U2 - 10.1021/acsnano.0c08136
DO - 10.1021/acsnano.0c08136
M3 - Article
C2 - 33512135
AN - SCOPUS:85100660544
SN - 1936-0851
VL - 15
SP - 6386
EP - 6394
JO - ACS Nano
JF - ACS Nano
IS - 4
ER -
