Codonopsis lanceolata Contributes to Ca 2+Homeostasis by Mediating SOCE and PLC/IP 3Pathways in Vascular Endothelial and Smooth Muscle Cells

Min Kyung Kim, A. Young Han, You Kyoung Shin, Kwang Won Lee, Geun Hee Seol

    Research output: Contribution to journalArticlepeer-review

    5 Citations (Scopus)

    Abstract

    Codonopsis lanceolata has been widely used as an anti-inflammatory and anti-lipogenic agent in traditional medicine. Recently, C. lanceolata was reported to prevent hypertension by improving vascular function. This study evaluated the effects of C. lanceolata and its major component lancemaside A on cytosolic calcium concentration in vascular endothelial cells and vascular smooth muscle cells. Cytosolic calcium concentration was measured using fura-2 AM fluorescence. C. lanceolata or lancemaside A increased the cytosolic calcium concentration by releasing Ca 2+from the endoplasmic reticulum and sarcoplasmic reticulum and by Ca 2+entry into endothelial cells and vascular smooth muscle cells from extracellular sources. The C. lanceolata - and lancemaside A-induced cytosolic calcium concentration increases were significantly inhibited by lanthanum, an inhibitor of non-selective cation channels, in both endothelial cells and vascular smooth muscle cells. Moreover, C. lanceolata and lancemaside A significantly inhibited store-operated Ca 2+entry under pathological extracellular Ca 2+levels. In Ca 2+-free extracellular fluid, increases in the cytosolic calcium concentration induced by C. lanceolata or lancemaside A were significantly inhibited by U73122, an inhibitor of phospholipase C, and 2-APB, an inositol 1,4,5-trisphosphate receptor antagonist. In addition, dantrolene treatment, which inhibits Ca 2+release through ryanodine receptor channels, also inhibited C. lanceolata - or lancemaside A-induced increases in the cytosolic calcium concentration through the phospholipase C/inositol 1,4,5-trisphosphate pathway. These results suggest that C. lanceolata and lancemaside A increase the cytosolic calcium concentration through the non-selective cation channels and phospholipase C/inositol 1,4,5-trisphosphate pathways under physiological conditions and inhibit store-operated Ca 2+entry under pathological conditions in endothelial cells and vascular smooth muscle cells. C. lanceolata or lancemaside A can protect endothelial cells and vascular smooth muscle cells by maintaining cytosolic calcium concentration homeostasis, suggesting possible applications for these materials in diets for preventing vascular damage.

    Original languageEnglish
    Pages (from-to)1345-1352
    Number of pages8
    JournalPlanta Medica
    Volume86
    Issue number18
    DOIs
    Publication statusPublished - 2020 Dec 1

    Bibliographical note

    Funding Information:
    This work was supported by a grant from the National Research Foundation of Korea Forest Service (KFS-2016005C10-1719-AB01) and Basic Science Research Program through the National Research Foundation of Korea (NRF-2018R1D1A1B07050048). This manuscript is a revision of M.K.K.’s masterʼs thesis from Korea University.

    Funding Information:
    This work was supported by a grant from the National Research Foundation of Korea Forest Service (KFS-2016005C10-1719-AB01) and Basic Science Research Program through the National Research Foundation of Korea (NRF-2018R1D1A1B07050048). This manuscript is a revision of M.K.K.'s master's thesis from Korea University.

    Publisher Copyright:
    © 2021 American Society of Civil Engineers (ASCE). All rights reserved.

    Keywords

    • Campanulaceae
    • Codonopsis lanceolate
    • inositol 1,4,5-trisphosphate
    • store-operated Ca entry
    • vascular endothelial cells
    • vascular smooth muscle cells

    ASJC Scopus subject areas

    • Analytical Chemistry
    • Molecular Medicine
    • Pharmacology
    • Pharmaceutical Science
    • Drug Discovery
    • Complementary and alternative medicine
    • Organic Chemistry

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