Cold shock response in Lactococcus lactis ssp. diacetylactis

Ki Beom Lee, Russell Cail, Seung Hyeon Moon, Man Bock Gu

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)


The acquired freeze-thaw tolerance was investigated for Lactococcus lactis ssp. diacetylactis. Pretreatment of microorganisms at less severe temperatures to initiate cold tolerance gave L. lactis ssp. diacetylactis improved cell viability after successive freezings and thawings. The ability of cells to survive freeze-thaw was dependent on factors experienced prior to freezing. Factors affecting lactic acid bacteria survival during freeze-thaw cycles were found to be different diluents, growth phase, and different cold temperatures. Viability experiments showed that this strain displaying cold shock cryotolerance had an improved survival capacity in stationary phase. The plasmid contents of lactic acid bacteria isolated from different types, DRC-2 and DRC-2C, were examined and compared with the plasmid contents of culture collection strains both before and after cold shock treatment. Using agarose gel electrophoresis, no obvious correlation between the cold shock response and the number of plasmids in the cell could be observed.

Original languageEnglish
Pages (from-to)93-97
Number of pages5
JournalBiotechnology and Bioprocess Engineering
Issue number2
Publication statusPublished - 1999
Externally publishedYes

Bibliographical note

Funding Information:
Acknowledgements This work was supported in part by the Korea Science and Engineering Foundation (KOSEF) through the ADvanced Environmental Monitoring Research Center (ADEMRC) at Kwangju Institute of Science and Technology (K-JIST).


  • Cold shock proteins
  • Cold shock response
  • Lactococcus lactis ssp. diacetylactis
  • Stationary phase

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology
  • Biomedical Engineering


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