Colorimetric allergenic fungal spore detection using peptide-modified gold nanoparticles

Joung In Lee; Seok Cheon Jang; Jinhyo Chung; Woong Ku Choi; Caleb Hong; Geum Ran Ahn; Seong Hwan Kim; Byung Yang Lee; Woo Jae Chung

doi:10.1016/j.snb.2020.128894

Colorimetric allergenic fungal spore detection using peptide-modified gold nanoparticles

Joung In Lee, Seok Cheon Jang, Jinhyo Chung, Woong Ku Choi, Caleb Hong, Geum Ran Ahn, Seong Hwan Kim, Byung Yang Lee, Woo Jae Chung

School of Mechanical Engineering

Research output: Contribution to journal › Article › peer-review

34 Citations (Scopus)

Abstract

The rapid and sensitive detection of allergenic fungal spores is of great interest owing to the potentially negative impact of these spores on personal and public health and safety. We present a simple and prompt colorimetric strategy for detecting Aspergillus niger based on interactions between fungal spores and gold nanoparticles (AuNPs) modified with a specific binding peptide. The A. niger spore-binding peptide ligand was identified by phage display screening, which exhibited enhanced affinity through multivalent effects with specificity over common airborne bacteria. Immobilization of peptide ligands on AuNPs enabled rapid binding to A. niger spores, resulting in a visible change in the color intensity of the supernatant after sedimentation of the spores. This simple colorimetric approach displayed a high sensitivity of ∼50 spores and a rapid detection time of <10 min when used with a smartphone-based image analysis application. This strategy can be adapted for the simple and rapid detection of target microorganisms of relatively large size by exploiting the specificity of ligands and the optical properties of gold nanoparticles.

Original language	English
Article number	128894
Journal	Sensors and Actuators, B: Chemical
Volume	327
DOIs	https://doi.org/10.1016/j.snb.2020.128894
Publication status	Published - 2021 Jan 15

Bibliographical note

Funding Information:
This research was supported by a National Research Foundation of Korea (NRF) grant, funded by the Ministry of Science, ICT and Future Planning ( NRF-2016M3A7B4909581 ), and by the Korean government (MIST) (No. 2019R1A2C1006856 ). This research was also supported by the Basic Science Research Program through the National Research Foundation of Korea (NRF) , funded by the Ministry of Education ( 2017R1A6A1A03015642 ). J.I.L. and S.C.J. contributed equally to this work.

Publisher Copyright:
© 2020 Elsevier B.V.

Keywords

Aspergillus niger spore
Colorimetric detection
Fungi sensor
Gold nanoparticle
Peptide ligand

ASJC Scopus subject areas

Electronic, Optical and Magnetic Materials
Instrumentation
Condensed Matter Physics
Surfaces, Coatings and Films
Metals and Alloys
Electrical and Electronic Engineering
Materials Chemistry

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.snb.2020.128894

Cite this

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title = "Colorimetric allergenic fungal spore detection using peptide-modified gold nanoparticles",

abstract = "The rapid and sensitive detection of allergenic fungal spores is of great interest owing to the potentially negative impact of these spores on personal and public health and safety. We present a simple and prompt colorimetric strategy for detecting Aspergillus niger based on interactions between fungal spores and gold nanoparticles (AuNPs) modified with a specific binding peptide. The A. niger spore-binding peptide ligand was identified by phage display screening, which exhibited enhanced affinity through multivalent effects with specificity over common airborne bacteria. Immobilization of peptide ligands on AuNPs enabled rapid binding to A. niger spores, resulting in a visible change in the color intensity of the supernatant after sedimentation of the spores. This simple colorimetric approach displayed a high sensitivity of ∼50 spores and a rapid detection time of <10 min when used with a smartphone-based image analysis application. This strategy can be adapted for the simple and rapid detection of target microorganisms of relatively large size by exploiting the specificity of ligands and the optical properties of gold nanoparticles.",

keywords = "Aspergillus niger spore, Colorimetric detection, Fungi sensor, Gold nanoparticle, Peptide ligand",

author = "Lee, {Joung In} and Jang, {Seok Cheon} and Jinhyo Chung and Choi, {Woong Ku} and Caleb Hong and Ahn, {Geum Ran} and Kim, {Seong Hwan} and Lee, {Byung Yang} and Chung, {Woo Jae}",

note = "Funding Information: This research was supported by a National Research Foundation of Korea (NRF) grant, funded by the Ministry of Science, ICT and Future Planning ( NRF-2016M3A7B4909581 ), and by the Korean government (MIST) (No. 2019R1A2C1006856 ). This research was also supported by the Basic Science Research Program through the National Research Foundation of Korea (NRF) , funded by the Ministry of Education ( 2017R1A6A1A03015642 ). J.I.L. and S.C.J. contributed equally to this work. Publisher Copyright: {\textcopyright} 2020 Elsevier B.V.",

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AU - Lee, Joung In

AU - Jang, Seok Cheon

AU - Chung, Jinhyo

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AU - Hong, Caleb

AU - Ahn, Geum Ran

AU - Kim, Seong Hwan

AU - Lee, Byung Yang

AU - Chung, Woo Jae

N1 - Funding Information: This research was supported by a National Research Foundation of Korea (NRF) grant, funded by the Ministry of Science, ICT and Future Planning ( NRF-2016M3A7B4909581 ), and by the Korean government (MIST) (No. 2019R1A2C1006856 ). This research was also supported by the Basic Science Research Program through the National Research Foundation of Korea (NRF) , funded by the Ministry of Education ( 2017R1A6A1A03015642 ). J.I.L. and S.C.J. contributed equally to this work. Publisher Copyright: © 2020 Elsevier B.V.

PY - 2021/1/15

Y1 - 2021/1/15

N2 - The rapid and sensitive detection of allergenic fungal spores is of great interest owing to the potentially negative impact of these spores on personal and public health and safety. We present a simple and prompt colorimetric strategy for detecting Aspergillus niger based on interactions between fungal spores and gold nanoparticles (AuNPs) modified with a specific binding peptide. The A. niger spore-binding peptide ligand was identified by phage display screening, which exhibited enhanced affinity through multivalent effects with specificity over common airborne bacteria. Immobilization of peptide ligands on AuNPs enabled rapid binding to A. niger spores, resulting in a visible change in the color intensity of the supernatant after sedimentation of the spores. This simple colorimetric approach displayed a high sensitivity of ∼50 spores and a rapid detection time of <10 min when used with a smartphone-based image analysis application. This strategy can be adapted for the simple and rapid detection of target microorganisms of relatively large size by exploiting the specificity of ligands and the optical properties of gold nanoparticles.

AB - The rapid and sensitive detection of allergenic fungal spores is of great interest owing to the potentially negative impact of these spores on personal and public health and safety. We present a simple and prompt colorimetric strategy for detecting Aspergillus niger based on interactions between fungal spores and gold nanoparticles (AuNPs) modified with a specific binding peptide. The A. niger spore-binding peptide ligand was identified by phage display screening, which exhibited enhanced affinity through multivalent effects with specificity over common airborne bacteria. Immobilization of peptide ligands on AuNPs enabled rapid binding to A. niger spores, resulting in a visible change in the color intensity of the supernatant after sedimentation of the spores. This simple colorimetric approach displayed a high sensitivity of ∼50 spores and a rapid detection time of <10 min when used with a smartphone-based image analysis application. This strategy can be adapted for the simple and rapid detection of target microorganisms of relatively large size by exploiting the specificity of ligands and the optical properties of gold nanoparticles.

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KW - Gold nanoparticle

KW - Peptide ligand

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Colorimetric allergenic fungal spore detection using peptide-modified gold nanoparticles

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

UN SDGs

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