Most animal cell culture media can be buffered using bicarbonate and high pressure CO2 in a closed system. However, in an open system, the pH of the culture media increases continuously due to the marked difference in CO2 pressure between the culture media and the atmosphere. Therefore, it is important to measure the exact pH of the culture media in an intact closed system. In this study, a pH measurement method was developed using visible light. The pH was calculated from light absorbance by the cells and by the culture media. This method was successfully applied to both suspension and anchorage-dependent cell cultures.
Bibliographical noteFunding Information:
Acknowledgements This work was supported by the Small and Medium Business Administration of Korea (Grant No. S0807222-E0843910-10100013) and the National Research Foundation of Korea (Grant No. N01090458).
- Animal cell culture
- Process analytical technology
- Process monitoring
- Visible light
- pH measurement
ASJC Scopus subject areas
- Applied Microbiology and Biotechnology