Colorimetric pH measurement of animal cell culture media

Juno Jang, Soo Jin Moon, Sung Hwan Hong, Ik Hwan Kim

    Research output: Contribution to journalArticlepeer-review

    15 Citations (Scopus)

    Abstract

    Most animal cell culture media can be buffered using bicarbonate and high pressure CO2 in a closed system. However, in an open system, the pH of the culture media increases continuously due to the marked difference in CO2 pressure between the culture media and the atmosphere. Therefore, it is important to measure the exact pH of the culture media in an intact closed system. In this study, a pH measurement method was developed using visible light. The pH was calculated from light absorbance by the cells and by the culture media. This method was successfully applied to both suspension and anchorage-dependent cell cultures.

    Original languageEnglish
    Pages (from-to)1599-1607
    Number of pages9
    JournalBiotechnology letters
    Volume32
    Issue number11
    DOIs
    Publication statusPublished - 2010

    Bibliographical note

    Funding Information:
    Acknowledgements This work was supported by the Small and Medium Business Administration of Korea (Grant No. S0807222-E0843910-10100013) and the National Research Foundation of Korea (Grant No. N01090458).

    Keywords

    • Animal cell culture
    • Process analytical technology
    • Process monitoring
    • Visible light
    • pH measurement

    ASJC Scopus subject areas

    • Biotechnology
    • Bioengineering
    • Applied Microbiology and Biotechnology

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