Colorimetric pH measurement of animal cell culture media

Juno Jang; Soo Jin Moon; Sung Hwan Hong; Ik Hwan Kim

doi:10.1007/s10529-010-0341-6

Colorimetric pH measurement of animal cell culture media

Juno Jang, Soo Jin Moon, Sung Hwan Hong, Ik Hwan Kim

Department of Biotechnology

Research output: Contribution to journal › Article › peer-review

10 Citations (Scopus)

Abstract

Most animal cell culture media can be buffered using bicarbonate and high pressure CO₂ in a closed system. However, in an open system, the pH of the culture media increases continuously due to the marked difference in CO₂ pressure between the culture media and the atmosphere. Therefore, it is important to measure the exact pH of the culture media in an intact closed system. In this study, a pH measurement method was developed using visible light. The pH was calculated from light absorbance by the cells and by the culture media. This method was successfully applied to both suspension and anchorage-dependent cell cultures.

Original language	English
Pages (from-to)	1599-1607
Number of pages	9
Journal	Biotechnology letters
Volume	32
Issue number	11
DOIs	https://doi.org/10.1007/s10529-010-0341-6
Publication status	Published - 2010

Bibliographical note

Funding Information:
Acknowledgements This work was supported by the Small and Medium Business Administration of Korea (Grant No. S0807222-E0843910-10100013) and the National Research Foundation of Korea (Grant No. N01090458).

Keywords

Animal cell culture
Process analytical technology
Process monitoring
Visible light
pH measurement

ASJC Scopus subject areas

Biotechnology
Bioengineering
Applied Microbiology and Biotechnology

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10.1007/s10529-010-0341-6

Cite this

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abstract = "Most animal cell culture media can be buffered using bicarbonate and high pressure CO2 in a closed system. However, in an open system, the pH of the culture media increases continuously due to the marked difference in CO2 pressure between the culture media and the atmosphere. Therefore, it is important to measure the exact pH of the culture media in an intact closed system. In this study, a pH measurement method was developed using visible light. The pH was calculated from light absorbance by the cells and by the culture media. This method was successfully applied to both suspension and anchorage-dependent cell cultures.",

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author = "Juno Jang and Moon, {Soo Jin} and Hong, {Sung Hwan} and Kim, {Ik Hwan}",

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T1 - Colorimetric pH measurement of animal cell culture media

AU - Jang, Juno

AU - Moon, Soo Jin

AU - Hong, Sung Hwan

AU - Kim, Ik Hwan

N1 - Funding Information: Acknowledgements This work was supported by the Small and Medium Business Administration of Korea (Grant No. S0807222-E0843910-10100013) and the National Research Foundation of Korea (Grant No. N01090458).

PY - 2010

Y1 - 2010

N2 - Most animal cell culture media can be buffered using bicarbonate and high pressure CO2 in a closed system. However, in an open system, the pH of the culture media increases continuously due to the marked difference in CO2 pressure between the culture media and the atmosphere. Therefore, it is important to measure the exact pH of the culture media in an intact closed system. In this study, a pH measurement method was developed using visible light. The pH was calculated from light absorbance by the cells and by the culture media. This method was successfully applied to both suspension and anchorage-dependent cell cultures.

AB - Most animal cell culture media can be buffered using bicarbonate and high pressure CO2 in a closed system. However, in an open system, the pH of the culture media increases continuously due to the marked difference in CO2 pressure between the culture media and the atmosphere. Therefore, it is important to measure the exact pH of the culture media in an intact closed system. In this study, a pH measurement method was developed using visible light. The pH was calculated from light absorbance by the cells and by the culture media. This method was successfully applied to both suspension and anchorage-dependent cell cultures.

KW - Animal cell culture

KW - Process analytical technology

KW - Process monitoring

KW - Visible light

KW - pH measurement

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AN - SCOPUS:77957963912

SN - 0141-5492

VL - 32

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EP - 1607

JO - Biotechnology letters

JF - Biotechnology letters

IS - 11

ER -

Colorimetric pH measurement of animal cell culture media

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

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