Abstract
Purpose. Our previous study confirmed that heparin-deoxycholic acid conjugate (HD) had a potent antiangiogenic effect and safety to use for long-term treatment. Herein, the combined therapeutic effect of HD and doxorubicin (DOX) was evaluated against squamous cell carcinoma (SCC7) and B16F10 melanoma. Methods. The inhibitory effect of cell proliferation and cellular uptake of HD was studied in SCC7 and B16F10. The combination effects of HD and DOX were evaluated by measuring cytotoxicity and apoptosis as well as tumor growth and apoptosis in vivo against SCC7 and B16F10 tumor-bearing mice. Results. HD displayed potent inhibitory effect on SCC7 and B16F10 cell proliferation, but it showed a low cytotoxic effect. Concurrent treatment of HD and DOX displayed enhanced cytotoxic effects and apoptosis on SCC7 and B16F10. The cellular uptake of HD and DOX was affected by the collective cytotoxic effects of these two drugs: each drug suppressed the tumor growth, and their combined treatment enhanced apoptosis and collectively inhibited the tumor growth of SCC7 and B16F10 in vivo. Conclusion. These results demonstrated that HD with cytostatic and antiangiogenetic activities, enhanced the antitumor activity of DOX against SCC7 and B16F10, and the combined treatment of these two drugs might have enhanced therapeutic efficacy.
| Original language | English |
|---|---|
| Pages (from-to) | 268-276 |
| Number of pages | 9 |
| Journal | Pharmaceutical research |
| Volume | 25 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - 2008 Feb |
| Externally published | Yes |
Bibliographical note
Funding Information:This study was supported by Next generation New Technology Development Program (Grant: # 10011353) of Ministry of Commerce, Industry and Energy in Korea.
Keywords
- Apoptosis
- B16F10
- Doxorubicin
- Heparin-deoxycholic acid conjugate
- SCC
ASJC Scopus subject areas
- Biotechnology
- Molecular Medicine
- Pharmacology
- Pharmaceutical Science
- Organic Chemistry
- Pharmacology (medical)