Combined effect of inorganic salts with calcium peroxide pretreatment for kenaf core biomass and their utilization for 2,3-butanediol production

Rijuta Ganesh Saratale; Han Seung Shin; Gajanan S. Ghodake; Gopalakrishnan Kumar; Min Kyu Oh; Ganesh Dattatraya Saratale

doi:10.1016/j.biortech.2018.02.115

Combined effect of inorganic salts with calcium peroxide pretreatment for kenaf core biomass and their utilization for 2,3-butanediol production

Rijuta Ganesh Saratale, Han Seung Shin, Gajanan S. Ghodake, Gopalakrishnan Kumar, Min Kyu Oh, Ganesh Dattatraya Saratale

Department of Chemical and Biological Engineering

Research output: Contribution to journal › Article › peer-review

22 Citations (Scopus)

Abstract

This study focuses on development of calcium peroxide (CaO₂) pretreatment that removes major part of lignin but retaining most of sugar components of kenaf core powder (KCP) biomass. In chemical pretreatment, usually higher loss of biomass occurs which was less during this pretreatment strategy. Supplementation of inorganic salts; manganese sulfate (MnSO₄) and cobalt chloride (COCl₂) in CaO₂ pretreatment resulted in maximum delignification of KCP relative to individual CaO₂ pretreatment. Maximum glucose yield (98%) and hydrolysis yield (80.5%) was achieved after enzymatic hydrolysis (30 FPU/g of KCP) under optimized conditions. Analytical results proved effective lignin removal and significant destruction of KCP with this pretreatment strategy. Finally, utilization of KCP enzymatic hydrolysates by developed strain Klebsiella pneumoniae KMK05 resulted in maximum 2,3-butanediol (BDO) production (10.42 g/L) and BDO titer (0.385 g/g of sugar). BDO titer achieved with KCP derived sugars were found comparable with the mixture of standard sugars which is notable.

Original language	English
Pages (from-to)	26-32
Number of pages	7
Journal	Bioresource technology
Volume	258
DOIs	https://doi.org/10.1016/j.biortech.2018.02.115
Publication status	Published - 2018 Jun

Bibliographical note

Funding Information:
This research work was completely supported by Dongguk University , Seoul, South Korea under research fund 2016–2018. One of the author GK is thankful to Korea Research Fellowship Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Science and ICT (Grant No: 2016H1D3A1908953). Appendix A

Publisher Copyright:
© 2018 Elsevier Ltd

Keywords

2,3-Butanediol production
Calcium peroxide pretreatment
Enzymatic hydrolysis
Kenaf core powder
Klebsiella pneumoniae KMK05

ASJC Scopus subject areas

Bioengineering
Environmental Engineering
Renewable Energy, Sustainability and the Environment
Waste Management and Disposal

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.biortech.2018.02.115

Cite this

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title = "Combined effect of inorganic salts with calcium peroxide pretreatment for kenaf core biomass and their utilization for 2,3-butanediol production",

abstract = "This study focuses on development of calcium peroxide (CaO2) pretreatment that removes major part of lignin but retaining most of sugar components of kenaf core powder (KCP) biomass. In chemical pretreatment, usually higher loss of biomass occurs which was less during this pretreatment strategy. Supplementation of inorganic salts; manganese sulfate (MnSO4) and cobalt chloride (COCl2) in CaO2 pretreatment resulted in maximum delignification of KCP relative to individual CaO2 pretreatment. Maximum glucose yield (98%) and hydrolysis yield (80.5%) was achieved after enzymatic hydrolysis (30 FPU/g of KCP) under optimized conditions. Analytical results proved effective lignin removal and significant destruction of KCP with this pretreatment strategy. Finally, utilization of KCP enzymatic hydrolysates by developed strain Klebsiella pneumoniae KMK05 resulted in maximum 2,3-butanediol (BDO) production (10.42 g/L) and BDO titer (0.385 g/g of sugar). BDO titer achieved with KCP derived sugars were found comparable with the mixture of standard sugars which is notable.",

keywords = "2,3-Butanediol production, Calcium peroxide pretreatment, Enzymatic hydrolysis, Kenaf core powder, Klebsiella pneumoniae KMK05",

author = "Saratale, {Rijuta Ganesh} and Shin, {Han Seung} and Ghodake, {Gajanan S.} and Gopalakrishnan Kumar and Oh, {Min Kyu} and Saratale, {Ganesh Dattatraya}",

note = "Funding Information: This research work was completely supported by Dongguk University , Seoul, South Korea under research fund 2016–2018. One of the author GK is thankful to Korea Research Fellowship Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Science and ICT (Grant No: 2016H1D3A1908953). Appendix A Publisher Copyright: {\textcopyright} 2018 Elsevier Ltd",

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language = "English",

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AU - Saratale, Rijuta Ganesh

AU - Shin, Han Seung

AU - Ghodake, Gajanan S.

AU - Kumar, Gopalakrishnan

AU - Oh, Min Kyu

AU - Saratale, Ganesh Dattatraya

N1 - Funding Information: This research work was completely supported by Dongguk University , Seoul, South Korea under research fund 2016–2018. One of the author GK is thankful to Korea Research Fellowship Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Science and ICT (Grant No: 2016H1D3A1908953). Appendix A Publisher Copyright: © 2018 Elsevier Ltd

PY - 2018/6

Y1 - 2018/6

N2 - This study focuses on development of calcium peroxide (CaO2) pretreatment that removes major part of lignin but retaining most of sugar components of kenaf core powder (KCP) biomass. In chemical pretreatment, usually higher loss of biomass occurs which was less during this pretreatment strategy. Supplementation of inorganic salts; manganese sulfate (MnSO4) and cobalt chloride (COCl2) in CaO2 pretreatment resulted in maximum delignification of KCP relative to individual CaO2 pretreatment. Maximum glucose yield (98%) and hydrolysis yield (80.5%) was achieved after enzymatic hydrolysis (30 FPU/g of KCP) under optimized conditions. Analytical results proved effective lignin removal and significant destruction of KCP with this pretreatment strategy. Finally, utilization of KCP enzymatic hydrolysates by developed strain Klebsiella pneumoniae KMK05 resulted in maximum 2,3-butanediol (BDO) production (10.42 g/L) and BDO titer (0.385 g/g of sugar). BDO titer achieved with KCP derived sugars were found comparable with the mixture of standard sugars which is notable.

AB - This study focuses on development of calcium peroxide (CaO2) pretreatment that removes major part of lignin but retaining most of sugar components of kenaf core powder (KCP) biomass. In chemical pretreatment, usually higher loss of biomass occurs which was less during this pretreatment strategy. Supplementation of inorganic salts; manganese sulfate (MnSO4) and cobalt chloride (COCl2) in CaO2 pretreatment resulted in maximum delignification of KCP relative to individual CaO2 pretreatment. Maximum glucose yield (98%) and hydrolysis yield (80.5%) was achieved after enzymatic hydrolysis (30 FPU/g of KCP) under optimized conditions. Analytical results proved effective lignin removal and significant destruction of KCP with this pretreatment strategy. Finally, utilization of KCP enzymatic hydrolysates by developed strain Klebsiella pneumoniae KMK05 resulted in maximum 2,3-butanediol (BDO) production (10.42 g/L) and BDO titer (0.385 g/g of sugar). BDO titer achieved with KCP derived sugars were found comparable with the mixture of standard sugars which is notable.

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Combined effect of inorganic salts with calcium peroxide pretreatment for kenaf core biomass and their utilization for 2,3-butanediol production

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

UN SDGs

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