TY - JOUR
T1 - Combined effect of inorganic salts with calcium peroxide pretreatment for kenaf core biomass and their utilization for 2,3-butanediol production
AU - Saratale, Rijuta Ganesh
AU - Shin, Han Seung
AU - Ghodake, Gajanan S.
AU - Kumar, Gopalakrishnan
AU - Oh, Min Kyu
AU - Saratale, Ganesh Dattatraya
N1 - Funding Information:
This research work was completely supported by Dongguk University , Seoul, South Korea under research fund 2016–2018. One of the author GK is thankful to Korea Research Fellowship Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Science and ICT (Grant No: 2016H1D3A1908953). Appendix A
Publisher Copyright:
© 2018 Elsevier Ltd
PY - 2018/6
Y1 - 2018/6
N2 - This study focuses on development of calcium peroxide (CaO2) pretreatment that removes major part of lignin but retaining most of sugar components of kenaf core powder (KCP) biomass. In chemical pretreatment, usually higher loss of biomass occurs which was less during this pretreatment strategy. Supplementation of inorganic salts; manganese sulfate (MnSO4) and cobalt chloride (COCl2) in CaO2 pretreatment resulted in maximum delignification of KCP relative to individual CaO2 pretreatment. Maximum glucose yield (98%) and hydrolysis yield (80.5%) was achieved after enzymatic hydrolysis (30 FPU/g of KCP) under optimized conditions. Analytical results proved effective lignin removal and significant destruction of KCP with this pretreatment strategy. Finally, utilization of KCP enzymatic hydrolysates by developed strain Klebsiella pneumoniae KMK05 resulted in maximum 2,3-butanediol (BDO) production (10.42 g/L) and BDO titer (0.385 g/g of sugar). BDO titer achieved with KCP derived sugars were found comparable with the mixture of standard sugars which is notable.
AB - This study focuses on development of calcium peroxide (CaO2) pretreatment that removes major part of lignin but retaining most of sugar components of kenaf core powder (KCP) biomass. In chemical pretreatment, usually higher loss of biomass occurs which was less during this pretreatment strategy. Supplementation of inorganic salts; manganese sulfate (MnSO4) and cobalt chloride (COCl2) in CaO2 pretreatment resulted in maximum delignification of KCP relative to individual CaO2 pretreatment. Maximum glucose yield (98%) and hydrolysis yield (80.5%) was achieved after enzymatic hydrolysis (30 FPU/g of KCP) under optimized conditions. Analytical results proved effective lignin removal and significant destruction of KCP with this pretreatment strategy. Finally, utilization of KCP enzymatic hydrolysates by developed strain Klebsiella pneumoniae KMK05 resulted in maximum 2,3-butanediol (BDO) production (10.42 g/L) and BDO titer (0.385 g/g of sugar). BDO titer achieved with KCP derived sugars were found comparable with the mixture of standard sugars which is notable.
KW - 2,3-Butanediol production
KW - Calcium peroxide pretreatment
KW - Enzymatic hydrolysis
KW - Kenaf core powder
KW - Klebsiella pneumoniae KMK05
UR - http://www.scopus.com/inward/record.url?scp=85042778573&partnerID=8YFLogxK
U2 - 10.1016/j.biortech.2018.02.115
DO - 10.1016/j.biortech.2018.02.115
M3 - Article
C2 - 29518688
AN - SCOPUS:85042778573
SN - 0960-8524
VL - 258
SP - 26
EP - 32
JO - Bioresource technology
JF - Bioresource technology
ER -
