Physical maps of chromosomes provide a framework for organizing and integrating diverse genetic information. DNA microarrays are a valuable technique for physical mapping and can also be used to facilitate the discovery of single feature polymorphisms (SFPs). Wheat chromosome arm 2BL was physically mapped using a Wheat Genome Array onto near-isogenic lines (NILs) with the aid of wheat-rice synteny and mapped wheat EST information. Using high variance probe set (HVP) analysis, 314 HVPs constituting genes present on 2BL were identified. The 314 HVPs were grouped into 3 categories: HVPs that match only rice chromosome 4 (298 HVPs), those that match only wheat ESTs mapped on 2BL (1), and those that match both rice chromosome 4 and wheat ESTs mapped on 2BL (15). All HVPs were converted into gene sets, which represented either unique rice gene models or mapped wheat ESTs that matched identified HVPs. Comparative physical maps were constructed for 16 wheat gene sets and 271 rice gene sets. Of the 271 rice gene sets, 257 were mapped to the 18-35 Mb regions on rice chromosome 4. Based on HVP analysis and sequence similarity between the gene models in the rice chromosomes and mapped wheat ESTs, the outermost rice gene model that limits the translocation breakpoint to orthologous regions was identified.
Bibliographical noteFunding Information:
Acknowledgments The authors would like to thank Dr. C. S. Jang and anonymous reviewers for very insightful feedback on this manuscript. This work was supported by a grant (No. 20070301-034-016-009-01-00) from BioGreen 21 Program, Rural Development Administration, Republic of Korea. This study was also supported by Technology Development Program for Agriculture and Forestry, Ministry for Agriculture, Forestry and Fisheries, Republic of Korea.
Copyright 2011 Elsevier B.V., All rights reserved.
- Comparative physical mapping
- High variance probe (HVP)
- Wheat chromosome arm 2BL
ASJC Scopus subject areas
- Animal Science and Zoology
- Plant Science
- Insect Science