Comparative proteomic analysis of virulent Korean Mycobacterium tuberculosis K-strain with other mycobacteria strain following infection of U-937 macrophage

Weon Ryoo Sung; Kil Park Young; Sue Nie Park; Soo Shim Young; Hyunjeong Liew; Seongman Kang; Gill Han Bai

Comparative proteomic analysis of virulent Korean Mycobacterium tuberculosis K-strain with other mycobacteria strain following infection of U-937 macrophage

Weon Ryoo Sung, Kil Park Young, Sue Nie Park, Soo Shim Young, Hyunjeong Liew, Seongman Kang, Gill Han Bai

Department of Life Sciences

Research output: Contribution to journal › Article › peer-review

15 Citations (Scopus)

Abstract

In Korea, the Mycobacterium tuberculosis K-strain is the most prevalent clinical isolates and belongs to the Beijing family. In this study, we conducted comparative porteomics of expressed proteins of clinical isolates of the K-strain with H37Rv, H37Ra as well as the vaccine strain of Mycobacterium bovis BCG following phagocytosis by the human monocytic cell line U-937. Proteins were analyzed by 2-D PAGE and MALDITOF-MS. Two proteins, Mb1363 (probable glycogen phosphorylase GlgP) and MT2656 (Haloalkane dehalogenase LinB) were most abundant after phagocytosis of M. tuberculosis K-strain. This approach provides a method to determine specific proteins that may have critical roles in tuberculosis pathogenesis.

Original language	English
Pages (from-to)	268-271
Number of pages	4
Journal	Journal of Microbiology
Volume	45
Issue number	3
Publication status	Published - 2007 Jun

Keywords

2-D PAGE
Human monocytic cell line U-937
MALDI-TOF-MS
MT2656
Mb1363
Mycobacterium tuberculosis K-strain
Proteomic analysis

ASJC Scopus subject areas

Microbiology
Applied Microbiology and Biotechnology

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Cite this

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title = "Comparative proteomic analysis of virulent Korean Mycobacterium tuberculosis K-strain with other mycobacteria strain following infection of U-937 macrophage",

abstract = "In Korea, the Mycobacterium tuberculosis K-strain is the most prevalent clinical isolates and belongs to the Beijing family. In this study, we conducted comparative porteomics of expressed proteins of clinical isolates of the K-strain with H37Rv, H37Ra as well as the vaccine strain of Mycobacterium bovis BCG following phagocytosis by the human monocytic cell line U-937. Proteins were analyzed by 2-D PAGE and MALDITOF-MS. Two proteins, Mb1363 (probable glycogen phosphorylase GlgP) and MT2656 (Haloalkane dehalogenase LinB) were most abundant after phagocytosis of M. tuberculosis K-strain. This approach provides a method to determine specific proteins that may have critical roles in tuberculosis pathogenesis.",

keywords = "2-D PAGE, Human monocytic cell line U-937, MALDI-TOF-MS, MT2656, Mb1363, Mycobacterium tuberculosis K-strain, Proteomic analysis",

author = "Sung, {Weon Ryoo} and Young, {Kil Park} and Park, {Sue Nie} and Young, {Soo Shim} and Hyunjeong Liew and Seongman Kang and Bai, {Gill Han}",

year = "2007",

month = jun,

language = "English",

volume = "45",

pages = "268--271",

journal = "Journal of Microbiology",

issn = "1225-8873",

publisher = "Microbiological Society of Korea",

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TY - JOUR

T1 - Comparative proteomic analysis of virulent Korean Mycobacterium tuberculosis K-strain with other mycobacteria strain following infection of U-937 macrophage

AU - Sung, Weon Ryoo

AU - Young, Kil Park

AU - Park, Sue Nie

AU - Young, Soo Shim

AU - Liew, Hyunjeong

AU - Kang, Seongman

AU - Bai, Gill Han

PY - 2007/6

Y1 - 2007/6

N2 - In Korea, the Mycobacterium tuberculosis K-strain is the most prevalent clinical isolates and belongs to the Beijing family. In this study, we conducted comparative porteomics of expressed proteins of clinical isolates of the K-strain with H37Rv, H37Ra as well as the vaccine strain of Mycobacterium bovis BCG following phagocytosis by the human monocytic cell line U-937. Proteins were analyzed by 2-D PAGE and MALDITOF-MS. Two proteins, Mb1363 (probable glycogen phosphorylase GlgP) and MT2656 (Haloalkane dehalogenase LinB) were most abundant after phagocytosis of M. tuberculosis K-strain. This approach provides a method to determine specific proteins that may have critical roles in tuberculosis pathogenesis.

AB - In Korea, the Mycobacterium tuberculosis K-strain is the most prevalent clinical isolates and belongs to the Beijing family. In this study, we conducted comparative porteomics of expressed proteins of clinical isolates of the K-strain with H37Rv, H37Ra as well as the vaccine strain of Mycobacterium bovis BCG following phagocytosis by the human monocytic cell line U-937. Proteins were analyzed by 2-D PAGE and MALDITOF-MS. Two proteins, Mb1363 (probable glycogen phosphorylase GlgP) and MT2656 (Haloalkane dehalogenase LinB) were most abundant after phagocytosis of M. tuberculosis K-strain. This approach provides a method to determine specific proteins that may have critical roles in tuberculosis pathogenesis.

KW - 2-D PAGE

KW - Human monocytic cell line U-937

KW - MALDI-TOF-MS

KW - MT2656

KW - Mb1363

KW - Mycobacterium tuberculosis K-strain

KW - Proteomic analysis

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M3 - Article

C2 - 17618234

AN - SCOPUS:34447124417

SN - 1225-8873

VL - 45

SP - 268

EP - 271

JO - Journal of Microbiology

JF - Journal of Microbiology

IS - 3

ER -

Comparative proteomic analysis of virulent Korean Mycobacterium tuberculosis K-strain with other mycobacteria strain following infection of U-937 macrophage

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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