Comparative proteomics using lipid over-producing or less-producing mutants unravels lipid metabolisms in Chlamydomonas reinhardtii

Yoon E. Choi; Hyemin Hwang; Hyun Soo Kim; Joon Woo Ahn; Won Joong Jeong; Ji Won Yang

doi:10.1016/j.biortech.2013.03.142

Comparative proteomics using lipid over-producing or less-producing mutants unravels lipid metabolisms in Chlamydomonas reinhardtii

Yoon E. Choi, Hyemin Hwang, Hyun Soo Kim, Joon Woo Ahn, Won Joong Jeong, Ji Won Yang

Research output: Contribution to journal › Article › peer-review

20 Citations (Scopus)

Abstract

In this study, proteomic approach was employed to advance our understanding of genes associated with microalgal lipid production. We first generated random mutants of Chlamydomonas reinhardtii, which displayed increased or decreased lipid content, compared to that in the wild-type. Mutants were designated as CR12 and CR48. Then, the changes of protein expression associated with the mutations of Chlamydomonas reinhardtii were analyzed. We could identify proteins that were significantly up-regulated or down-regulated either in the wild-type or CR12 or CR48. Our results will help understand additional genes or pathways directly or indirectly linked to microalgal lipid production.

Original language	English
Pages (from-to)	108-115
Number of pages	8
Journal	Bioresource technology
Volume	145
DOIs	https://doi.org/10.1016/j.biortech.2013.03.142
Publication status	Published - 2013 Oct
Externally published	Yes

Keywords

Chlamydomonas reinhardtii
Comparative proteomics
Microalgal lipid biosynthesis
Random mutagenesis

ASJC Scopus subject areas

Bioengineering
Environmental Engineering
Renewable Energy, Sustainability and the Environment
Waste Management and Disposal

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.biortech.2013.03.142

Cite this

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title = "Comparative proteomics using lipid over-producing or less-producing mutants unravels lipid metabolisms in Chlamydomonas reinhardtii",

abstract = "In this study, proteomic approach was employed to advance our understanding of genes associated with microalgal lipid production. We first generated random mutants of Chlamydomonas reinhardtii, which displayed increased or decreased lipid content, compared to that in the wild-type. Mutants were designated as CR12 and CR48. Then, the changes of protein expression associated with the mutations of Chlamydomonas reinhardtii were analyzed. We could identify proteins that were significantly up-regulated or down-regulated either in the wild-type or CR12 or CR48. Our results will help understand additional genes or pathways directly or indirectly linked to microalgal lipid production.",

keywords = "Chlamydomonas reinhardtii, Comparative proteomics, Microalgal lipid biosynthesis, Random mutagenesis",

author = "Choi, {Yoon E.} and Hyemin Hwang and Kim, {Hyun Soo} and Ahn, {Joon Woo} and Jeong, {Won Joong} and Yang, {Ji Won}",

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doi = "10.1016/j.biortech.2013.03.142",

language = "English",

volume = "145",

pages = "108--115",

journal = "Bioresource technology",

issn = "0960-8524",

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T1 - Comparative proteomics using lipid over-producing or less-producing mutants unravels lipid metabolisms in Chlamydomonas reinhardtii

AU - Choi, Yoon E.

AU - Hwang, Hyemin

AU - Kim, Hyun Soo

AU - Ahn, Joon Woo

AU - Jeong, Won Joong

AU - Yang, Ji Won

PY - 2013/10

Y1 - 2013/10

N2 - In this study, proteomic approach was employed to advance our understanding of genes associated with microalgal lipid production. We first generated random mutants of Chlamydomonas reinhardtii, which displayed increased or decreased lipid content, compared to that in the wild-type. Mutants were designated as CR12 and CR48. Then, the changes of protein expression associated with the mutations of Chlamydomonas reinhardtii were analyzed. We could identify proteins that were significantly up-regulated or down-regulated either in the wild-type or CR12 or CR48. Our results will help understand additional genes or pathways directly or indirectly linked to microalgal lipid production.

AB - In this study, proteomic approach was employed to advance our understanding of genes associated with microalgal lipid production. We first generated random mutants of Chlamydomonas reinhardtii, which displayed increased or decreased lipid content, compared to that in the wild-type. Mutants were designated as CR12 and CR48. Then, the changes of protein expression associated with the mutations of Chlamydomonas reinhardtii were analyzed. We could identify proteins that were significantly up-regulated or down-regulated either in the wild-type or CR12 or CR48. Our results will help understand additional genes or pathways directly or indirectly linked to microalgal lipid production.

KW - Chlamydomonas reinhardtii

KW - Comparative proteomics

KW - Microalgal lipid biosynthesis

KW - Random mutagenesis

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Comparative proteomics using lipid over-producing or less-producing mutants unravels lipid metabolisms in Chlamydomonas reinhardtii

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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