Comparative study of P19 EC stem cell differentiation in between conventional hanging drop and the zebrafish chorion as a bio-derived material

Seok Na Dae, Hwang Lee, Uk Kim Sun, Nam Hwang Chang, Ho Lee Sang, Yoon Kang Ji, Kyeong Kim Jai, Jungho Pak James

    Research output: Contribution to journalArticlepeer-review

    1 Citation (Scopus)

    Abstract

    Various materials including glass and polymers have been widely used for stem cell culture due to their biocompatibility. However, the roles of these materials are fundamentally limited because they cannot realize or imitate the complex biological functions of living tissues, except in very simple cases. Here, the development of a bio-derived material suitable for stem cell culture and improvement of differentiation efficiency to specific cell lineages with no stimulating agents by using a chorion obtained from a fertilized zebrafish egg through the removal of the yolk and embryonic cell mass from the egg is reported. Mouse P19 EC stem cells introduced into the empty chorion form a uniform embryoid body (EB) without addition of any inducing agent. It is demonstrated that the zebrafish chorion with nanopores improves efficiencies greatly in the EB formation, cell proliferation, and lineage-specific differentiations compared to those of the conventional hanging drop culture method.

    Original languageEnglish
    Pages (from-to)73-84
    Number of pages12
    JournalJournal of Biomaterials Applications
    Volume23
    Issue number1
    DOIs
    Publication statusPublished - 2008 Jul

    Bibliographical note

    Copyright:
    Copyright 2008 Elsevier B.V., All rights reserved.

    Keywords

    • A bio-derived material
    • Comparison
    • Defferentiation
    • Hanging drop
    • P19 EC stem cell
    • Zebrafish chorion

    ASJC Scopus subject areas

    • Biomaterials
    • Biomedical Engineering

    Fingerprint

    Dive into the research topics of 'Comparative study of P19 EC stem cell differentiation in between conventional hanging drop and the zebrafish chorion as a bio-derived material'. Together they form a unique fingerprint.

    Cite this