TY - JOUR
T1 - Comparative transcriptome analysis of Haematococcus pluvialis on astaxanthin biosynthesis in response to irradiation with red or blue LED wavelength
AU - Lee, Changsu
AU - Ahn, Joon Woo
AU - Kim, Jin Baek
AU - Kim, Jee Young
AU - Choi, Yoon E.
N1 - Funding Information:
Fig. 7 Confirmation of isopentenyl pyrophosphate isomerase (IPI) and carotenoid hydroxylase (CHY) gene expressions in H. pluvialis induced by blue and red wavelength irradiation Acknowledgements This work was supported by the National Research Foundation of Korea (NRF) Grant funded by the Korea government (MSIP) (2016R1D1A1B03932773); and the Korea Basic Science Institute under the R&D program (Project No. C38703), supervised by the Ministry of Science, ICT and Future Planning; and the Academic Research Program funded by Astabio Co., Ltd. (Jeonju, Korea). This work was also supported by the research program of KAERI, Republic of Korea.
Funding Information:
This work was supported by the National Research Foundation of Korea (NRF) Grant funded by the Korea government (MSIP) (2016R1D1A1B03932773); and the Korea Basic Science Institute under the R&D program (Project No. C38703), supervised by the Ministry of Science, ICT and Future Planning; and the Academic Research Program funded by Astabio Co., Ltd. (Jeonju, Korea). This work was also supported by the research program of KAERI, Republic of Korea. The authors have no conflict of interest to declare.
Publisher Copyright:
© 2018, Springer Science+Business Media B.V., part of Springer Nature.
PY - 2018/7/1
Y1 - 2018/7/1
N2 - The unicellular green microalga Haematococcus pluvialis has the highest content of the natural antioxidant, astaxanthin. Previously, it was determined that astaxanthin accumulation in H. pluvialis could be induced by blue-wavelength irradiation; however, the molecular mechanism remains unknown. The present study aimed to compare the transcriptome of H. pluvialis, with respect to astaxanthin biosynthesis, under the monochromatic red (660 nm) or blue (450 nm) light-emitting diode (LED) irradiation. Among a total of 165,372 transcripts, we identified 67,703 unigenes, of which 2245 and 171 were identified as differentially expressed genes (DEGs) in response to blue and red irradiation, respectively. Interestingly, expressional changes of blue light receptor cryptochromes were detected in response to blue and/or red LED irradiation in H. pluvialis, which may directly and indirectly regulate astaxanthin biosynthesis. In accordance with this observation, expression of the BKT and CHY genes, which are part of the downstream section of the astaxanthin biosynthetic pathway, was significantly upregulated by blue LED irradiation compared with their expression under control white irradiation. Contrastingly, they were downregulated by red LED irradiation. Our transcriptome study provided molecular insights that highlighted the different of responses of H. pluvialis to red and blue irradiation, especially for astaxanthin biosynthesis.
AB - The unicellular green microalga Haematococcus pluvialis has the highest content of the natural antioxidant, astaxanthin. Previously, it was determined that astaxanthin accumulation in H. pluvialis could be induced by blue-wavelength irradiation; however, the molecular mechanism remains unknown. The present study aimed to compare the transcriptome of H. pluvialis, with respect to astaxanthin biosynthesis, under the monochromatic red (660 nm) or blue (450 nm) light-emitting diode (LED) irradiation. Among a total of 165,372 transcripts, we identified 67,703 unigenes, of which 2245 and 171 were identified as differentially expressed genes (DEGs) in response to blue and red irradiation, respectively. Interestingly, expressional changes of blue light receptor cryptochromes were detected in response to blue and/or red LED irradiation in H. pluvialis, which may directly and indirectly regulate astaxanthin biosynthesis. In accordance with this observation, expression of the BKT and CHY genes, which are part of the downstream section of the astaxanthin biosynthetic pathway, was significantly upregulated by blue LED irradiation compared with their expression under control white irradiation. Contrastingly, they were downregulated by red LED irradiation. Our transcriptome study provided molecular insights that highlighted the different of responses of H. pluvialis to red and blue irradiation, especially for astaxanthin biosynthesis.
KW - Carotenoid biosynthesis
KW - Differentially expressed genes
KW - Gene expression
KW - LED wavelength
KW - Microalgae
UR - http://www.scopus.com/inward/record.url?scp=85048810691&partnerID=8YFLogxK
U2 - 10.1007/s11274-018-2459-y
DO - 10.1007/s11274-018-2459-y
M3 - Article
C2 - 29916185
AN - SCOPUS:85048810691
SN - 0959-3993
VL - 34
JO - World Journal of Microbiology and Biotechnology
JF - World Journal of Microbiology and Biotechnology
IS - 7
M1 - 96
ER -