Comparison of proteomes between wheat-rye translocations and their recurrent parents

T. G. Lee; J. W. Johnson; Y. W. Seo

doi:10.1134/S1021443711030095

Comparison of proteomes between wheat-rye translocations and their recurrent parents

T. G. Lee, J. W. Johnson, Y. W. Seo

Research output: Contribution to journal › Article › peer-review

2 Citations (Scopus)

Abstract

2BS. 2RL wheat-rye translocations have presented phenotypes of improved agronomic performance, such as Hessian fly resistance. The main objective of this work was to use two-dimensional electrophoresis to identify the proteomic differences between 2BS.2RL wheat-rye translocations and their recurrent parents. The investigation of seeds revealed line-specific protein spots, such as α-amylase inhibitor 0.19. More diverse expression patterns were observed in leaves than in seeds. Protein spots found specifically in 2BS.2RL, but not in non-2RL translocations were identified as β-glucosidase and vacuolar ATP synthase subunit B2, demonstrating the effects of translocated rye chromatin 2RL on common wheat genetic background. When the leaf protein spots were compared in the control and Hessian fly-infested near-isogenic line (NIL) (2BS.2RL), many down-regulated proteins and specific proteins, such as β-glucosidase, were detected in the latter.

Original language	English
Pages (from-to)	703-714
Number of pages	12
Journal	Russian Journal of Plant Physiology
Volume	58
Issue number	4
DOIs	https://doi.org/10.1134/S1021443711030095
Publication status	Published - 2011 Jul

Bibliographical note

Funding Information:
ACKNOWLEDGMENTS The authors would like to thank Dr. S.E. Cambron (USDA ARS, Department of Entomology, Purdue University, IN, USA) and Mr. D.E. Bland (Depart ment of Crop and Soil Sciences, University of Geor gia, Griffin Campus, GA, USA) for their assistance with Hessian fly infestation. The present work was supported by a grant (no. 20070301 034 016 009 01 00) from the BioGreen 21 Program, Rural Develop ment Administration, Republic of Korea. This study was also supported by the Technology Development Program for Agriculture and Forestry, Ministry for Agriculture, Forestry and Fisheries, Republic of Korea.

Keywords

2BS.2RL wheat-rye translocationsHessian fly
proteome analysis

ASJC Scopus subject areas

Plant Science

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10.1134/S1021443711030095

Cite this

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title = "Comparison of proteomes between wheat-rye translocations and their recurrent parents",

abstract = "2BS. 2RL wheat-rye translocations have presented phenotypes of improved agronomic performance, such as Hessian fly resistance. The main objective of this work was to use two-dimensional electrophoresis to identify the proteomic differences between 2BS.2RL wheat-rye translocations and their recurrent parents. The investigation of seeds revealed line-specific protein spots, such as α-amylase inhibitor 0.19. More diverse expression patterns were observed in leaves than in seeds. Protein spots found specifically in 2BS.2RL, but not in non-2RL translocations were identified as β-glucosidase and vacuolar ATP synthase subunit B2, demonstrating the effects of translocated rye chromatin 2RL on common wheat genetic background. When the leaf protein spots were compared in the control and Hessian fly-infested near-isogenic line (NIL) (2BS.2RL), many down-regulated proteins and specific proteins, such as β-glucosidase, were detected in the latter.",

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author = "Lee, {T. G.} and Johnson, {J. W.} and Seo, {Y. W.}",

note = "Funding Information: ACKNOWLEDGMENTS The authors would like to thank Dr. S.E. Cambron (USDA ARS, Department of Entomology, Purdue University, IN, USA) and Mr. D.E. Bland (Depart ment of Crop and Soil Sciences, University of Geor gia, Griffin Campus, GA, USA) for their assistance with Hessian fly infestation. The present work was supported by a grant (no. 20070301 034 016 009 01 00) from the BioGreen 21 Program, Rural Develop ment Administration, Republic of Korea. This study was also supported by the Technology Development Program for Agriculture and Forestry, Ministry for Agriculture, Forestry and Fisheries, Republic of Korea.",

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N2 - 2BS. 2RL wheat-rye translocations have presented phenotypes of improved agronomic performance, such as Hessian fly resistance. The main objective of this work was to use two-dimensional electrophoresis to identify the proteomic differences between 2BS.2RL wheat-rye translocations and their recurrent parents. The investigation of seeds revealed line-specific protein spots, such as α-amylase inhibitor 0.19. More diverse expression patterns were observed in leaves than in seeds. Protein spots found specifically in 2BS.2RL, but not in non-2RL translocations were identified as β-glucosidase and vacuolar ATP synthase subunit B2, demonstrating the effects of translocated rye chromatin 2RL on common wheat genetic background. When the leaf protein spots were compared in the control and Hessian fly-infested near-isogenic line (NIL) (2BS.2RL), many down-regulated proteins and specific proteins, such as β-glucosidase, were detected in the latter.

AB - 2BS. 2RL wheat-rye translocations have presented phenotypes of improved agronomic performance, such as Hessian fly resistance. The main objective of this work was to use two-dimensional electrophoresis to identify the proteomic differences between 2BS.2RL wheat-rye translocations and their recurrent parents. The investigation of seeds revealed line-specific protein spots, such as α-amylase inhibitor 0.19. More diverse expression patterns were observed in leaves than in seeds. Protein spots found specifically in 2BS.2RL, but not in non-2RL translocations were identified as β-glucosidase and vacuolar ATP synthase subunit B2, demonstrating the effects of translocated rye chromatin 2RL on common wheat genetic background. When the leaf protein spots were compared in the control and Hessian fly-infested near-isogenic line (NIL) (2BS.2RL), many down-regulated proteins and specific proteins, such as β-glucosidase, were detected in the latter.

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Comparison of proteomes between wheat-rye translocations and their recurrent parents

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

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