Comparison of the toxicity of aluminum oxide nanorods with different aspect ratio

Eun Jung Park; Gwang Hee Lee; Jae hun Shim; Myung Haing Cho; Byoung Seok Lee; Yong Bum Kim; Jae Ho Kim; Younghun Kim; Dong Wan Kim

doi:10.1007/s00204-014-1332-5

Comparison of the toxicity of aluminum oxide nanorods with different aspect ratio

Eun Jung Park, Gwang Hee Lee, Jae hun Shim, Myung Haing Cho, Byoung Seok Lee, Yong Bum Kim, Jae Ho Kim, Younghun Kim, Dong Wan Kim

Research output: Contribution to journal › Article › peer-review

27 Citations (Scopus)

Abstract

Aluminum oxide nanoparticles are listed among 14 high-priority nanomaterials published by the Organization for Economic Co-operation and Development, but limited information is available on their potential hazards. In this study, we compared the toxicity of two different aluminum oxide nanorods (AlNRs) commercially available in vivo and in vitro. Considering aspect ratio, one was 6.2 ± 0.6 (long-AlNRs) and the other was 2.1 ± 0.4 (short-AlNRs). In mice, long-AlNRs induced longer and stronger inflammatory responses than short-AlNRs, and the degree reached the maximum on day 7 for both types and decreased with time. In addition, in vitro tests were performed on six cell lines derived from potential target organs for AlNPs, HEK-293 (kidney), HACAT (skin), Chang (liver), BEAS-2B (lung), T98G (brain), and H9C2 (heart), using MTT assay, ATP assay, LDH release, and xCELLigence system. Long-AlNRs generally produced stronger toxicity than short-AlNRs, and HEK-293 cells were the most sensitive for both AlNRs, followed by BEAS-2B cells, although results from 4 kinds of toxicity tests conflicted among the cell lines. Based on these results, we suggest that toxicity of AlNRs may be related to aspect ratio (and resultant surface area). Furthermore, novel in vitro toxicity testing methods are needed to resolve questionable results caused by the unique properties of nanoparticles.

Original language	English
Pages (from-to)	1771-1782
Number of pages	12
Journal	Archives of Toxicology
Volume	89
Issue number	10
DOIs	https://doi.org/10.1007/s00204-014-1332-5
Publication status	Published - 2015 Oct 24

Bibliographical note

Funding Information:
This work was supported by the Basic Science Research Program through the National Research Foundation of Korea funded by the Ministry of Education, Science and Technology (NRF-2011-35B-E00011). And, this research was supported by Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Science, ICT and future Planning (2012R1A2A2A01045382).

Publisher Copyright:
© 2014, Springer-Verlag Berlin Heidelberg.

Keywords

Aluminum oxide nanoparticles
Aspect ratio
In vitro system
Nanorods
Toxicity

ASJC Scopus subject areas

Toxicology
Health, Toxicology and Mutagenesis

Access to Document

10.1007/s00204-014-1332-5

Cite this

@article{aa5345069caa47dd87b28d7b68c6bdcb,

title = "Comparison of the toxicity of aluminum oxide nanorods with different aspect ratio",

abstract = "Aluminum oxide nanoparticles are listed among 14 high-priority nanomaterials published by the Organization for Economic Co-operation and Development, but limited information is available on their potential hazards. In this study, we compared the toxicity of two different aluminum oxide nanorods (AlNRs) commercially available in vivo and in vitro. Considering aspect ratio, one was 6.2 ± 0.6 (long-AlNRs) and the other was 2.1 ± 0.4 (short-AlNRs). In mice, long-AlNRs induced longer and stronger inflammatory responses than short-AlNRs, and the degree reached the maximum on day 7 for both types and decreased with time. In addition, in vitro tests were performed on six cell lines derived from potential target organs for AlNPs, HEK-293 (kidney), HACAT (skin), Chang (liver), BEAS-2B (lung), T98G (brain), and H9C2 (heart), using MTT assay, ATP assay, LDH release, and xCELLigence system. Long-AlNRs generally produced stronger toxicity than short-AlNRs, and HEK-293 cells were the most sensitive for both AlNRs, followed by BEAS-2B cells, although results from 4 kinds of toxicity tests conflicted among the cell lines. Based on these results, we suggest that toxicity of AlNRs may be related to aspect ratio (and resultant surface area). Furthermore, novel in vitro toxicity testing methods are needed to resolve questionable results caused by the unique properties of nanoparticles.",

keywords = "Aluminum oxide nanoparticles, Aspect ratio, In vitro system, Nanorods, Toxicity",

author = "Park, {Eun Jung} and Lee, {Gwang Hee} and Shim, {Jae hun} and Cho, {Myung Haing} and Lee, {Byoung Seok} and Kim, {Yong Bum} and Kim, {Jae Ho} and Younghun Kim and Kim, {Dong Wan}",

note = "Funding Information: This work was supported by the Basic Science Research Program through the National Research Foundation of Korea funded by the Ministry of Education, Science and Technology (NRF-2011-35B-E00011). And, this research was supported by Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Science, ICT and future Planning (2012R1A2A2A01045382). Publisher Copyright: {\textcopyright} 2014, Springer-Verlag Berlin Heidelberg.",

year = "2015",

month = oct,

day = "24",

doi = "10.1007/s00204-014-1332-5",

language = "English",

volume = "89",

pages = "1771--1782",

journal = "Archives of Toxicology",

issn = "0340-5761",

publisher = "Springer Verlag",

number = "10",

}

TY - JOUR

T1 - Comparison of the toxicity of aluminum oxide nanorods with different aspect ratio

AU - Park, Eun Jung

AU - Lee, Gwang Hee

AU - Shim, Jae hun

AU - Cho, Myung Haing

AU - Lee, Byoung Seok

AU - Kim, Yong Bum

AU - Kim, Jae Ho

AU - Kim, Younghun

AU - Kim, Dong Wan

N1 - Funding Information: This work was supported by the Basic Science Research Program through the National Research Foundation of Korea funded by the Ministry of Education, Science and Technology (NRF-2011-35B-E00011). And, this research was supported by Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Science, ICT and future Planning (2012R1A2A2A01045382). Publisher Copyright: © 2014, Springer-Verlag Berlin Heidelberg.

PY - 2015/10/24

Y1 - 2015/10/24

N2 - Aluminum oxide nanoparticles are listed among 14 high-priority nanomaterials published by the Organization for Economic Co-operation and Development, but limited information is available on their potential hazards. In this study, we compared the toxicity of two different aluminum oxide nanorods (AlNRs) commercially available in vivo and in vitro. Considering aspect ratio, one was 6.2 ± 0.6 (long-AlNRs) and the other was 2.1 ± 0.4 (short-AlNRs). In mice, long-AlNRs induced longer and stronger inflammatory responses than short-AlNRs, and the degree reached the maximum on day 7 for both types and decreased with time. In addition, in vitro tests were performed on six cell lines derived from potential target organs for AlNPs, HEK-293 (kidney), HACAT (skin), Chang (liver), BEAS-2B (lung), T98G (brain), and H9C2 (heart), using MTT assay, ATP assay, LDH release, and xCELLigence system. Long-AlNRs generally produced stronger toxicity than short-AlNRs, and HEK-293 cells were the most sensitive for both AlNRs, followed by BEAS-2B cells, although results from 4 kinds of toxicity tests conflicted among the cell lines. Based on these results, we suggest that toxicity of AlNRs may be related to aspect ratio (and resultant surface area). Furthermore, novel in vitro toxicity testing methods are needed to resolve questionable results caused by the unique properties of nanoparticles.

AB - Aluminum oxide nanoparticles are listed among 14 high-priority nanomaterials published by the Organization for Economic Co-operation and Development, but limited information is available on their potential hazards. In this study, we compared the toxicity of two different aluminum oxide nanorods (AlNRs) commercially available in vivo and in vitro. Considering aspect ratio, one was 6.2 ± 0.6 (long-AlNRs) and the other was 2.1 ± 0.4 (short-AlNRs). In mice, long-AlNRs induced longer and stronger inflammatory responses than short-AlNRs, and the degree reached the maximum on day 7 for both types and decreased with time. In addition, in vitro tests were performed on six cell lines derived from potential target organs for AlNPs, HEK-293 (kidney), HACAT (skin), Chang (liver), BEAS-2B (lung), T98G (brain), and H9C2 (heart), using MTT assay, ATP assay, LDH release, and xCELLigence system. Long-AlNRs generally produced stronger toxicity than short-AlNRs, and HEK-293 cells were the most sensitive for both AlNRs, followed by BEAS-2B cells, although results from 4 kinds of toxicity tests conflicted among the cell lines. Based on these results, we suggest that toxicity of AlNRs may be related to aspect ratio (and resultant surface area). Furthermore, novel in vitro toxicity testing methods are needed to resolve questionable results caused by the unique properties of nanoparticles.

KW - Aluminum oxide nanoparticles

KW - Aspect ratio

KW - In vitro system

KW - Nanorods

KW - Toxicity

UR - http://www.scopus.com/inward/record.url?scp=84942118502&partnerID=8YFLogxK

U2 - 10.1007/s00204-014-1332-5

DO - 10.1007/s00204-014-1332-5

M3 - Article

C2 - 25155191

AN - SCOPUS:84942118502

SN - 0340-5761

VL - 89

SP - 1771

EP - 1782

JO - Archives of Toxicology

JF - Archives of Toxicology

IS - 10

ER -

Comparison of the toxicity of aluminum oxide nanorods with different aspect ratio

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this