Comparison of three multiplex PCR assays for detection of respiratory viruses: Anyplex II RV16, AdvanSure RV, and Real-Q RV

Seung Gyu Yun, Min Young Kim, Jong Moon Choi, Chang Kyu Lee, Chae Seung Lim, Yunjung Cho, In Bum Suh

Research output: Contribution to journalArticlepeer-review

16 Citations (Scopus)


Background: Due to its great sensitivity, the nucleic acid amplification test (NAAT) is widely used for detection of respiratory viruses (RV). However, few reports have described a direct comparison between multiplex RT-PCR assays for RV. The objective of this study was to perform a direct comparison of three multiplex RT-PCR assays for the detection of respiratory viruses. Methods: A total of 201 respiratory samples (161 nasopharyngeal swab samples and 40 sputum samples) were tested with three commercial RV assays: Seegene Anyplex II RV16 (AP), LG AdvanSure RV (AD), and Biosewoom Real-Q RV (RQ). The additional tests for the discrepant results were conducted by repeat RV assay or monoplex PCR coupled direct sequencing. Data analysis using percent agreement, kappa, and prevalence-adjusted and bias-adjusted kappa (PABAK) values was performed for comparisons among the three RV assays. Results: Of the 201 samples, AP, AD, and RQ detected 105 (52.2%), 99 (49.3%), and 95 (47.3%) positive cases respectively. The overall agreement, kappa, and PABAK values for the three assays ranged between 97%-98%, 0.76-0.86, and 0.93-0.96 respectively. The performance of the three assays was very similar, with 94%-100% agreement for all comparisons, each virus types. The additional testing of samples showed discrepant results demonstrating that AD assay had the highest rate of concordance with original results. Conclusions: We suggest that all multiplex assay would be suitable for the detection of for respiratory viruses in clinical setting.

Original languageEnglish
Article numbere22230
JournalJournal of Clinical Laboratory Analysis
Issue number2
Publication statusPublished - 2018 Feb

Bibliographical note

Publisher Copyright:
© 2017 The Authors Journal of Clinical Laboratory Analysis Published by Wiley Periodicals, Inc.


  • agreement
  • nucleic acid amplification test
  • respiratory virus

ASJC Scopus subject areas

  • Immunology and Allergy
  • Hematology
  • Public Health, Environmental and Occupational Health
  • Clinical Biochemistry
  • Medical Laboratory Technology
  • Biochemistry, medical
  • Microbiology (medical)


Dive into the research topics of 'Comparison of three multiplex PCR assays for detection of respiratory viruses: Anyplex II RV16, AdvanSure RV, and Real-Q RV'. Together they form a unique fingerprint.

Cite this