Complete Genome Sequencing of Polar Arthrobacter sp. PAMC25284, Copper Tolerance Potential Unraveled with Genomic Analysis

Jayram Karmacharya, Prasansah Shrestha, So Ra Han, Hyun Park, Tae Jin Oh

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)

Abstract

The genus Arthrobacter is a known group of Gram-positive, opportunistic pathogenic bacteria from cold climates, with members that are believed to play a variety of roles at low temperatures. However, their survival mechanisms in frigid environments like the Antarctic are still unknown. We identified a species of Arthrobacter isolated from seawater in the polar region using 16S rRNA sequence analysis. The strain PAMC25284 genome consists of a circular chromosome with a GC content of 65.6% and is projected to contain 3,588 genes, of which 3,150 are protein coding, 366 are pseudogenes, 19 are rRNA coding, and 50 are tRNA coding genes. Using comparative genomics, we showed that PMAC25284 has copper-transporting ATPases, copper chaperone, copper-responsive transcriptional regulator, and multi-copper oxidase domains, which are found in both Gram-positive (like Mycobacterium tuberculosis and Enterococcus hirae) and Gram-negative bacteria (like E. coli and Pseudomonas aeruginosa). The existence of 4 multi-copper oxidase genes, which supplied an additional copper defense mechanism, could be intriguing information regarding Gram-positive bacteria such as Arthrobacter sp. PAMC25284. In addition, our strain PAMC25284 has the same MmcO gene as M. tuberculosis, with a locus tag KY499_RS04055 similarity of 40.61%, which is the highest among the Gram-positive and Gram-negative bacteria studied for this gene. Our cold-adapted Arthrobacter sp. strain PAMC25564 was published previously but did not contain a multi-copper oxidase domain-containing gene, but strain PAMC25284 was studied in this study.

Original languageEnglish
Article number1162938
JournalInternational Journal of Microbiology
Volume2022
DOIs
Publication statusPublished - 2022

Bibliographical note

Publisher Copyright:
© 2022 Jayram Karmacharya et al.

ASJC Scopus subject areas

  • Microbiology
  • Microbiology (medical)

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